, a New Species of Avian Origin Free

Abstract

A mycoplasma, designated strain 486, was isolated from the oviduct of an adult chicken. On the basis of its morphological, physical and cultural characteristics the organism was assigned to the class , order The guanine plus cytosine content of its DNA was estimated to be 27·5 mol%. The organism was inhibited by digitonin and it showed a growth response to sterol, although its minimal requirement was low. It neither showed helical forms nor hydrolysed urea and was hence assigned to the family , genus Strain 486 fermented glucose and reduced tetrazolium under anaerobic conditions. It did not hydrolyse arginine, aesculin or arbutin, nor did it produce films and spots or digest serum. Phosphatase activity was negative or very weak, and the organism adsorbed turkey but not chicken red blood cells. Serological tests (growth inhibition, indirect fluorescent antibody, double immunodiffusion and metabolism inhibition) with 75 currently accepted species or serovars failed to identify the isolate. Thus strain 486 appears to represent a new species, for which the name is proposed. (NCTC 10194, ATCC 35277).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-3-597
1984-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/3/mic-130-3-597.html?itemId=/content/journal/micro/10.1099/00221287-130-3-597&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittler R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. International Journal of Systematic Bacteriology 20:35–58
    [Google Scholar]
  2. Askaa G., Christiansen C., Ernø H. 1973; Bovine mycoplasmas: genome size and base composition of DNA. Journal of General Microbiology 75:283–286
    [Google Scholar]
  3. Black F. T. 1973; Modifications of the growth inhibition test and its application to human T- mycoplasmas. Applied Microbiology 25:528–533
    [Google Scholar]
  4. Bradbury J. M. 1977; Rapid biochemical tests for characterization of the Mycoplasmatales . Journal of Clinical Microbiology 5:531–534
    [Google Scholar]
  5. Bradbury J. M., Jordan F.T.W. 1972; Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination reactions. Journal of Hygiene 70:267–278
    [Google Scholar]
  6. Bradbury J. M., Forrest M., Williams A. 1983; Mycoplasma lipofaciens, a new species of avian origin. International Journal of Systematic Bacteriology 33:329–335
    [Google Scholar]
  7. Domermuth C. H., Nielsen M. H., Freundt E. A., Birch-Anderson A. 1964; Ultrastructure of Mycoplasma species. Journal of Bacteriology 88:727–744
    [Google Scholar]
  8. Edward D.G.FF. 1971; Determination of sterol requirement for Mycoplasmatales . Journal of General Microbiology 69:205–210
    [Google Scholar]
  9. Edward D. G. FF., Moore W. B. 1975; A method for determining the utilisation of glucose by Mycoplasmas. Journal of Medical Microbiology 8:451–454
    [Google Scholar]
  10. Ernø H., Stipkovits L. 1973; Bovine mycoplas-mas: cultural and biochemical studies. I. Acta veterinaria scandinavica 14:436–449
    [Google Scholar]
  11. Forrest M., Bradbury J. M. 1982; Studies on some unclassified avian mycoplasmas. Reviews of Infectious Diseases 4, May-June Supplement p. S272
    [Google Scholar]
  12. Freundt E. A., Andrews B. E., Ernø H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanethole-sulfonate and digitonin. Zentralblatt für Bakteriologie, Parasi- tenkunde, Infektionskrankheiten und Hygiene (Abtei- lung I, Originate A) 225:104–112
    [Google Scholar]
  13. Furness G., Demaggio M., Whitescarver J. 1975; Morphological changes induced during fixation of Mycoplasma mycoides for electron microscopy. Texas Reports on Biology and Medicine 33:415–422
    [Google Scholar]
  14. Gourlay R. N., Wyld S. G., Leach R. H. 1977; Mycoplasma alvi, a new species from bovine intestinal and urogenital tracts. International Journal of Systematic Bacteriology 27:86–96
    [Google Scholar]
  15. Hollingdale M. R., Lemcke R. M. 1969; The antigens of Mycoplasma hominis . Journal of Hygiene 67:585–602
    [Google Scholar]
  16. Howard C. J., Gourlay R. N. 1974; An electron- microscopic examination of certain bovine mycoplasmas stained with ruthenium red and the demonstration of a capsule on Mycoplasma dispar . Journal of General Microbiology 83:393–398
    [Google Scholar]
  17. International Committee on Systematic Bacteriology, Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes . International Journal of Systematic Bacteriology 29:172–180
    [Google Scholar]
  18. Jordan F. T. W., Amin M. M. 1980; A survey of mycoplasma infections in domestic poultry. Research in Veterinary Science 28:96–100
    [Google Scholar]
  19. Jordan F. T. W., Ernø H., Cottew G. S., Hinz K. H., Stipkovits L. 1982; Characterization and taxonomic description of five mycoplasma serovars (serotypes) of avian origin and their elevation to species rank and further evaluation of the taxonomic status of Mycoplasma synoviae . International Journal of Systematic Bacteriology 32:108–115
    [Google Scholar]
  20. Kelton W. H., Mandel M. 1969; Deoxyribonucleic acid base compositions of mycoplasma strains of avian origin. Journal of General Microbiology 56:131–135
    [Google Scholar]
  21. Langford E. V., Leach R. H. 1973; Characterization of a mycoplasma isolated from infectious bovine keratoconjunctivitis: M. bovoculi sp. nov. Canadian Journal of Microbiology 19:1435–1444
    [Google Scholar]
  22. Maniloff J., Morowitz H. J. 1972; Cell biology of the mycoplasmas. Bacteriological Reviews 36:263–290
    [Google Scholar]
  23. Razin S., Tully J. G. 1970; Cholesterol require-ment of mycoplasmas. Journal of Bacteriology 102:306–310
    [Google Scholar]
  24. Robertson J., Gomersall M., Gill P. 1975; Mycoplasma hominis: growth, reproduction and isolation of small viable cells. Journal of Bacteriology 124:1007–1018
    [Google Scholar]
  25. Rosendal S., Black F. T. 1972; Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta pathologica et microbiolo- gica scandinavica B80:615–622
    [Google Scholar]
  26. Sobeslavsky O., Prescott B., Chanock R. M. 1968; Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. Journal of Bacteriology 96:695–705
    [Google Scholar]
  27. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. Journal of Hygiene 64:91–104
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-3-597
Loading
/content/journal/micro/10.1099/00221287-130-3-597
Loading

Data & Media loading...

Most cited Most Cited RSS feed