Strains of containing informational suppressors were grown on medium containing antibiotics known to affect protein synthesis at the ribosomal level. These strains reacted in the anticipated manner: presumed ribosomal suppressors and were sensitive or even hypersensitive to aminoglycoside antibiotics, whereas presumed tRNA-like suppressors and were only slightly sensitive or wild-type in response. Hygromycin and paromomycin were the most useful antibiotics. All the antibiotics reduced the colony radial growth rate, , increased the lag phase and produced wrinkled morphology. Hygromycin was the most toxic. Resistant sectors were produced on paromomycin and hygromycin. The selective action of ‘misreading’ antibiotics on and strains is further evidence that these are ribosomal suppressors, whereas and may code for altered tRNA molecules. The results imply that hygromycin or paromomycin could be used for isolating ribosomal suppressors.


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