1887

Abstract

A cationic polymer of -galactosamine was isolated from culture filtrates of a colonial temperature-sensitive strain of Adsorption of the polymer to the cell surface initiated immediate efflux of low molecular weight metabolites and subsequent loss of viability. The polymer appeared to bind to those sites on the cell surface that normally bind calcium ions. Chemical analysis of the polymer showed it to be partially -acetylated. The polymer had an isoelectric point of 8·4. Thirty percent of the -galactosamine residues contained free amino groups. A rapid assay that has potential application for monitoring the effect of a variety of other membrane-active factors on membrane permeability has been developed.

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1984-03-01
2021-08-04
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References

  1. Ames B. N. 1966; Assay of inorganic phosphate, total phosphate and phosphatases. Methods in Enzymology 2:115–118
    [Google Scholar]
  2. Davidson E. A. 1966; Analysis of sugars found in mucopolysaccharides. Methods in Enzymology 8:52–60
    [Google Scholar]
  3. Debusk R. M., Debusk A. G. 1980; Physiological and regulatory properties of the general amino acid transport system of Neurospora crassa. Journal of Bacteriology 143:188–197
    [Google Scholar]
  4. Debusk R. M., Brown D. T., Debusk A. G., Penderghast R. D. 1981; Alternate mechanism for amino acid entry into Neurosporacrassa: extracellular deamination and subsequent keto acid transport. Journal of Bacteriology 146:163–169
    [Google Scholar]
  5. Dische Z., Borenfreund E. 1950; A spectropho- tometric method for the microdetermination of hexosamines. Journal of Biological Chemistry 184:517–522
    [Google Scholar]
  6. Distler J. J., Roseman S. 1960; Galactosamine polymers produced by Aspergillus parasiticus. Journal of Biological Chemistry 235:2538–2541
    [Google Scholar]
  7. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith R. 1956; Colorimetric method for the determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  8. Glasgow J. E., Reissig J. L. 1974; Interaction of galactosaminoglycan with Neurospora conidia. Journal of Bacteriology 120:759–766
    [Google Scholar]
  9. Habeeb A. F. S. A. 1966; Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. Analytical Chemistry 14:328–336
    [Google Scholar]
  10. Harold F. M. 1962; Binding of inorganic polyphosphate to the cell wall of Neurospora crassa. Biochimica et biophysica acta 57:59–66
    [Google Scholar]
  11. Jaspers H. T. A., Christianse K., Van Steveninck J. 1975; An improved method for the preparation of yeast enzymes in situ. Biochemical and Biophysical Research Communications 65:1434–1439
    [Google Scholar]
  12. Lehnhardt W. F., Winzler R. J. 1968; Determination of neutral sugars in glycoproteins by gas-liquid chromatography. Journal of Chromatography 34:471–479
    [Google Scholar]
  13. Lindahl U., Backstrom G., Jansson L., Hallen A. 1973; Biosynthesis of heparin II.Formation of sulfamino groups. Journal of Biological Chemistry 248:7234–7241
    [Google Scholar]
  14. Ludowieg J., Benmaman D. J. 1967; Colorimeter differentiation of hexosamines. Analytical Biochemistry 19:80–88
    [Google Scholar]
  15. Mayhew E., Harlos J. P., Juliano R. L. 1973; The effect of polycations on cell membrane stability and transport processes. Journal of Membrane Biology 14:213–228
    [Google Scholar]
  16. Quinton P. M., Philpott C. W. 1973; A role for anionic sites in epithelial architecture. Effects of cationic polymers on cell membrane structure. Journal of Cell Biology 56:787–796
    [Google Scholar]
  17. Reissig J. L., Glasgow J. E. 1971; Mucopolysaccharide which regulates growth in Neurospora. Journal of Bacteriology 190:882–889
    [Google Scholar]
  18. Sela M., Katchalski A. 1959; Biological properties of poly-α-amino acids. Advances in Protein Chemistry 14:391–478
    [Google Scholar]
  19. Sirica A. E., Woodman R. J. 1971; Selective aggregation of L-1210 leukemia cells by the polycation chitosan. Journal of the National Cancer Institute 47:377–388
    [Google Scholar]
  20. Spackman D. S. 1964; Automated amino acid analysis. Federation Proceedings 23:37
    [Google Scholar]
  21. Springer W. D., Srb A. M. 1978; Molecular alteration in a Neurospora crassa morphological mutant and its phenocopy. Proceedings of the National Academy of Sciences of the United States of America 75:1461–1465
    [Google Scholar]
  22. Takata M., Pickard W. F., Lettvin J. Y., Moore J. W. 1966; Ionic conductance changes in the lobster axon membrane when lanthanum is substituted for calcium. Journal of General Physiology 50:461–471
    [Google Scholar]
  23. Tisdale J. H., Debusk A. G. 1970; Developmental regulation of amino acid transport in Neurospora crassa. Journal of Bacteriology 104:689–697
    [Google Scholar]
  24. Tsuji J., Kinoshita T., Hoshino M. 1969; Analytical chemical studies on amino sugars. II. Determination of hexosamines using 3-methyl-2-benzothiazolonehydrazone hydrochloride. Chemical and Pharmaceutical Bulletin 17:1505–1510
    [Google Scholar]
  25. Vesterburg O. 1972; Isoelectric focusing. Methods in Enzymology 22:389–412
    [Google Scholar]
  26. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–446
    [Google Scholar]
  27. Weber P., Winzler R. J. 1969; Determination of hexosaminitols by ion exchange chromatography and its application to alkali labile glycosidic linkages in glycoproteins. Archives of Biochemistry and Biophysics 129:534–538
    [Google Scholar]
  28. Weiss G. B. 1974; Cellular pharmacology of lanthanum. Annual Review of Pharmacology 14:343–354
    [Google Scholar]
  29. Yphantis D. A., Dainko J. L., Schlenk F. 1967; Effect of some proteins on the yeast cell membrane. Journal of Bacteriology 94:1509–1515
    [Google Scholar]
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