1887

Abstract

When heat activated spores were incubated at room temperature a rapid and transient increase in cyclic AMP content was observed. A similar increase was observed during the heat treatment itself but the cyclic AMP content had returned to the value of dormant spores at the end of the 5 min treatment. The cyclic AMP content increased only after treatments at temperatures which also induced spore germination. A more gradual rise in cyclic AMP was also found during activation at room temperature with acetate and after activation of the spores with pyrosulphite. The increase in cyclic AMP content is the earliest event known to occur after activation of the spores. It clearly preceded the increase in trehalase activity observed during and/or after all the activating treatments, suggesting that, as shown for activation, the activation of trehalase is due to a cyclic AMP-dependent phosphorylation. An increase in cyclic AMP was a common feature of all activation methods investigated and appears to be the factor triggering spore germination.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-3-541
1984-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/3/mic-130-3-541.html?itemId=/content/journal/micro/10.1099/00221287-130-3-541&mimeType=html&fmt=ahah

References

  1. Borchert R. 1962; Über die Azetat-Aktivierung der Sporangiosporen von Phycomyces blakesleeanus. Beiträge zur Biologie der Pflanzen 38:31–36
    [Google Scholar]
  2. Delvaux E. 1973; Some aspects of germination induction in Phycomyces blakesleeanusby an ammonium acetate pretreatment. Archiv für Mikrobiologie 88:273–284
    [Google Scholar]
  3. Mazon M. J., Gancedo J. M., Gancedo C. 1982a; Inactivation of yeast fructose-1,6-bisphosphatase. Journal of Biological Chemistry 257:1128–1130
    [Google Scholar]
  4. Mazon M. J., Gancedo J. M., Gancedo C. 1982b; Phosphorylation and inactivation of yeast fructose-bisphosphatase in vivo by glucose and by proton ionophores.A possible role for cAMP. European Journal of Biochemistry 127:605–608
    [Google Scholar]
  5. Orlowski M. 1980; Cyclic adenosine 3′,5′-mono-phosphate and germination of sporangiospores from the fungus Mucor. Archives of Microbiology 126:133–140
    [Google Scholar]
  6. Ortiz C. H., Maia J. C. C., Tenan M. N., Brazpadrao G. R., Mattoon J. R., Panek A. D. 1983; Regulation of yeast trehalase by a monocyclic, cyclic AMP-dependent phosphorylation-dephosphorylation cascade system. Journal of Bacteriology 153:644–651
    [Google Scholar]
  7. Pall M. L. 1977; Cyclic AMP and the plasma membrane potential in Neurospora crassa. Journal of Biological Chemistry 252:7146–7150
    [Google Scholar]
  8. Paznokas J. L., Sypherd P. S. 1975; Respiratory capacity, cyclic adenosine 3′,5′-monophosphate, and morphogenesis ofMucor racemosus. Journal of Bacteriology 124:134–139
    [Google Scholar]
  9. Purwin C., Leidig F., Holzer H. 1982; Cyclic AMP-dependent phosphorylation of fructose-1,6- bisphosphatase in yeast. Biochemical and Biophysical Research Communications 107:1482–1489
    [Google Scholar]
  10. Robbins W. J., Kavanagh V. W., Kavanagh F. 1942; Growth substances and dormancy of spores of Phycomyces. Botanical Gazette 104:224–242
    [Google Scholar]
  11. Rosenberg G., Pall M. L. 1978; Cyclic AMP and cyclic GMP in germinating conidia of Neurospora crassa. Archives of Microbiology 118:87–90
    [Google Scholar]
  12. Rudolph H. 1958; Entwicklungsphysiologische Untersuchungen an den Sporangiophoren von Phycomyces blakesleeanus. Biologisches Zentralblatt 77:385–437
    [Google Scholar]
  13. Rudolph H., Ochsen B. 1969; Trehalose-Umsatz wärmeaktivierter Sporen von Phycomyces blakesleeanus. Archiv für Mikrobiologie 65:163–171
    [Google Scholar]
  14. Thevelein J. M., Van Assche J. A., Heremans K. 1980; Effect of high pressure on the heat activation in vivoof trehalase in the spores of Phycomyces blakesleeanus. European Journal of Biochemistry 111:171–175
    [Google Scholar]
  15. Thevelein J. M., Van Laere A. J., Beullens M., Van Assche J. A., Carlier A. R. 1983; Glucose-induced trehalase activation and trehalose mobilization during early germination of Phycomyces blakesleeanus spores. Journal of General Microbiology 129:719–726
    [Google Scholar]
  16. Tovey K. C., Oldham K. G., Whelan J. A. M. 1974; A simple direct assay for cyclic AMP in plasma and other biological samples using an improved competitive protein binding technique. Clinica chimica acta 56:221–234
    [Google Scholar]
  17. Trevillyan J. M., Pall M. L. 1979; Control of cyclic adenosine 3′,5′-monophosphate levels by depolarizing agents in fungi. Journal of Bacteriology 138:397–403
    [Google Scholar]
  18. Uno I., Ishikawa T. 1981; Control of adenosine 3′,5′-monophosphate level and protein phosphorylation by depolarizing agents in Coprinus macrorhizus. Biochimica et biophysica acta 672:108–113
    [Google Scholar]
  19. Vale V. L., Gomes S. L., Maia J. C. C., Mennucci L. 1976; Transient cyclic AMP accumulation in germinating zoospores of Blastocladiella emersonii. FEBS Letters 67:189–192
    [Google Scholar]
  20. Van Assche J. A., Carlier A. R., Dekeersmaeker H. I. 1972; Trehalase activity in dormant and activated spores of Phycomyces blakesleeanus. Planta 103:327–333
    [Google Scholar]
  21. Van Assche J. A., Carlier A. R., Van Tieghem L. L. C. 1977; The effect of gamma radiation on breaking of dormancy in Phycomycesspores. Archives of Microbiology 113:95–97
    [Google Scholar]
  22. Van Assche J. A., Van Laere A. J., Carlier A. R. 1978; Trehalose metabolism in dormant and activated spores of Phycomyces blakesleeanus Burgeff. Planta 139:171–176
    [Google Scholar]
  23. Van Der Plaat J. B., Van Solingen P. 1974; Cyclic 3′,5′-adenosine monophosphate stimulates trehalose degradation in baker’s yeast. Biochemical and Biophysical Research Communications 56:580–587
    [Google Scholar]
  24. Van Laere A. J., Hendrix P. 1983; Cyclic AMP-dependent in vitroactivation of trehalase from dormant Phycomyces blakesleeanusspores. Journal of General Microbiology 129:3287–3290
    [Google Scholar]
  25. Van Laere A. J., Van Assche J. A., Carlier A. R. 1980a; Reversible and irreversible activation of Phycomyces blakesleeanusspores. Experimental Mycology 4:96–104
    [Google Scholar]
  26. Van Laere A. J., Van Assche J. A., Carlier A. R. 1980b; Metabolism and chemical activation of Phycomyces blakesleeanusspores. Experimental Mycology 4:260–268
    [Google Scholar]
  27. Van Laere A. J., Van Schaftingen E., Hers H.-G. 1983; Fructose 2,6-bisphosphate and germination of fungal spores. Proceedings of the National Academy of Sciences of the United States of America 80:6601–6605
    [Google Scholar]
  28. Van Solingen P., Van Der Plaat J. B. 1975; Partial purification of the protein system controlling the breakdown of trehalose in baker’s yeast. Biochemical and Biophysical Research Communications 62:553–560
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-3-541
Loading
/content/journal/micro/10.1099/00221287-130-3-541
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error