1887

Abstract

The hydrogenation of -linolenic acid to stearic acid which occurs with the mixed bacteria of the sheep rumen can be demonstrated by mixing exponential phase cultures of only two species of rumen bacteria and incubating for a further period with α-linolenic acid. One bacterium must be able to hydrogenate α-linolenic acid to predominantly -octadec-11-enoic acid which is then used 11 substrate by a second bacterium. Cultures grown from small mixed inocula failed, with the exception of one pair of bacteria, to hydrogenate α-linolenic acid to stearic acid. The products from these cultures showed that one of the pair of bacteria had outgrown the other. For stearate production it was necessary to use inocula with a minimum number of cells rather than cells in a particular phase of growth. Two of the bacteria used, P2/2 and T344, after several years in pure culture show an increased isomerization of the octadecenoic acid products.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-3-527
1984-03-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/3/mic-130-3-527.html?itemId=/content/journal/micro/10.1099/00221287-130-3-527&mimeType=html&fmt=ahah

References

  1. Blaxter K. L. 1957; Myopathic conditions in animals. Veterinary Record 69:1150–1155
    [Google Scholar]
  2. Body D. R. 1976; The occurrence of cis-octadec-15-enoic acid as a major hydrogenation product of methyl linolenate in bovine rumen liquor. Biochemical Journal 157:741–744
    [Google Scholar]
  3. Carroll K. K. 1961; Quantitative estimation of peak areas in gas-liquid chromatography. Nature; London: 191377–378
    [Google Scholar]
  4. Dawson R. M. C., Kemp P. 1970; Biohydrogenation of dietary fats in ruminants. In Physiology of Digestion and Metabolism in the Ruminant pp. 504–518 Phillipson A. T. Edited by Newcastle upon Tyne, Oriel Press;
    [Google Scholar]
  5. Hazlewood G. P., Dawson R. M. C. 1979; Characteristics of a lipolytic and fatty acid-requiring Butyrivibrio sp. isolated from the ovine rumen. Journal of General Microbiology 112:15–27
    [Google Scholar]
  6. Hazlewood G. P., Kemp P., Lander D., Dawson R. M. C. 1976; C18 unsaturated fatty acid hydrogenation patterns of some rumen bacteria and their ability to hydrolyse exogenous phospholipid. British Journal of Nutrition 35:293–297
    [Google Scholar]
  7. Hazlewood G. P., Reynolds M. J., Dawson R. M. C., Gunstone F. D. 1979; An automatic colorimeter and its use in evaluating the growth response of an anaerobic general fatty acid auxotroph to cis- and trans-octadecenoic acids. Journal of Applied Bacteriology 47:321–325
    [Google Scholar]
  8. Hungate R. E. 1969; A role tube method for the cultivation of strict anaerobes. Methods of Microbiology 3B:117–132
    [Google Scholar]
  9. Kemp P., Dawson R. M. C. 1968; Isomerisation of linolenic acid by rumen micro-organisms. Biochemical Journal 109:477–478
    [Google Scholar]
  10. Kemp P., White R. W., Lander D. J. 1975; The hydrogenation of unsaturated fatty acids by five bacterial isolates from the sheep rumen, including a new species. Journal of General Microbiology 90:100–114
    [Google Scholar]
  11. Kepler C. R., Tove S. B. 1967; Biohydrogenation of fatty acids. III. Purification and properties of linoleate △12-cis-△11-trans-isomerase from Butyrivibrio fibrisolvens. Journal of Biological Chemistry 242:5686–5692
    [Google Scholar]
  12. Kepler C. R., Hirons K. P., Mcneill J. J., Tove S. B. 1966; Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens. Journal of Biological Chemistry 241:1350–1354
    [Google Scholar]
  13. Klein R. A., Hazlewood G. P., Kemp P., Dawson R. M. C. 1979; A new series of long-chain dicarboxylic acids with vicinal dimethyl branching found as major components of the lipids of Butyri vibrio spp. Biochemical Journal 183:691–700
    [Google Scholar]
  14. Latham M. J., Sharpe E. M. 1971; The isolation of anaerobic organisms from the bovine rumen. In Isolation of Anaerobes pp. 133–147 Shapton D. A., Board R. G. Edited by London:: Academic Press;
    [Google Scholar]
  15. Nieman C. 1954; Influence of trace amounts of fatty acids on the growth of microorganisms. Bacteriological Reviews 18:147–167
    [Google Scholar]
  16. White R. W., Mackenzie H. R., Bousfield I. J. 1974; Successful freeze-drying and retention of biohydrogenation activity of bacteria isolated from the bovine rumen. Journal of Applied Bacteriology 37:vi
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-3-527
Loading
/content/journal/micro/10.1099/00221287-130-3-527
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error