Interactions of Yeast Cells, Germ Tubes and Hyphae with Human Polymorphonuclear Leucocytes Free

Abstract

Suspensions of yeast cells, germ tubes and hyphae with biomass standardized by ATP measurement were compared for their relative susceptibilities to phagocytosis and intracellular killing by human polymorphonuclear leucocytes. All three forms were ingested to a similar extent, but significantly fewer yeast cells were killed intracellularly after ingestion than were filamentous forms of the fungus. Ketoconazole pretreatment significantly enhanced the susceptibility of hyphae, but not of germ tubes, to phagocytosis and intracellular killing. The opsonic requirements of the yeasts and filamentous forms for efficient phagocytosis and killing differed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-3-465
1984-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/3/mic-130-3-465.html?itemId=/content/journal/micro/10.1099/00221287-130-3-465&mimeType=html&fmt=ahah

References

  1. Bridges C. G., Dasilva G. L., Yamamura M., Valdimarsson H. 1980; A radiometric assay for the combined measurement of phagocytosis and intracellular killing of Candida albicans. Clinical and Experimental Immunology 42:226–233
    [Google Scholar]
  2. Chattaway F. W., O’Reilly J., Barlow A. J. E, Aldersley T. 1976; Induction of the mycelial form of Candida albicans by hydrolysates of peptides from seminal plasma. Journal of General Microbiology 95:335–347
    [Google Scholar]
  3. De Brabander M., Aerts F., Van Cutsem J., Van Den Bossche H., Borgers M. 1980; The activity of ketoconazole in mixed cultures of leukocytes and Candida albicans. Sabouraudia 18:197–210
    [Google Scholar]
  4. Denning T. J. V., Davies R. R. 1973; Candida albicans and the chemotaxis of polymorphonuclear neutrophils. Sabouraudia 11:210–221
    [Google Scholar]
  5. Diamond R. D., Krzesicki R., Jao W. 1978; Damage to pseudohyphal forms of Candida albicansby neutrophils in the absence of serum in vitro. Journal of Clinical Investigation 61:349–359
    [Google Scholar]
  6. Gow N. A. R., Gooday G. W., Newsam R. J., Gull K. 1980; Ultrastructure of the septum in Candida albicans. Current Microbiology 4:357–359
    [Google Scholar]
  7. Holm-Hansen O. 1973; Empirical counting of colonies with different numbers of cells per colony. In Estuarine Microbial Ecology pp. 73–89 Stevenson L. H., Caldwell R. R. Edited by Columbia:: University of South Carolina Press;
    [Google Scholar]
  8. Kagaya K., Fukazawa Y. 1981; Murine defense mechanism against Candida albicans infection. II. Opsonisation, phagocytosis and intracellular killing of C. albicans. Microbiology and Immunology 25:807–818
    [Google Scholar]
  9. Louria D. B., Brayton R. G. 1964; Behavior of Candida cells within leukocytes. Proceedings of the Society for Experimental Biology and Medicine 115:93–98
    [Google Scholar]
  10. Odds F. C. 1980; Laboratory evaluation of antifungal agents: a comparative study of imidazole derivatives of clinical importance. Journal of Antimicrobial Chemotherapy 6:749–761
    [Google Scholar]
  11. Odds F. C., Evans E. G. V., Holland K. T. 1975; Detection of Candida precipitins.A com-parison of double diffusion and counter immunoelectrophoresis. Journal of Immunological Methods 7:211–218
    [Google Scholar]
  12. Odds F. C., Milne L. J. R., Gentles J. C., Ball E. H. 1980; The activity in vitro and in vivo of a new imidazole antifungal, ketoconazole. Journal of Antimicrobial Chemotherapy 6:97–104
    [Google Scholar]
  13. Richardson M. D., Smith H. 1981; Resistance of virulent and attenuated strains of Candida albicans to intracellular killing by human and mouse phagocytes. Journal of Infectious Diseases 144:565–569
    [Google Scholar]
  14. Rogers J. T., Balish E. 1980; Immunity to Candida albicans. Microbiological Reviews 44:660–682
    [Google Scholar]
  15. Rogers K. B. 1966; Candida infections in paediatrics. In Symposium on Candida Infections pp. 179–195 Winner H., Hurley R. Edited by London:: Livingstone;
    [Google Scholar]
  16. Sasada M., Johnston R. B. 1980; Macrophage microbicidal activity: correlation between phagocytosis-associated oxidative metabolism and the killing of Candida by macrophages. Journal of Experimental Medicine 152:85–98
    [Google Scholar]
  17. Scherwitz C., Martin R. 1979; The phagocytosis of Candida albicansblastospores and germ tubes by polymorphonuclear leukocytes. Dermatologica 159:12–23
    [Google Scholar]
  18. Scherwitz C., Martin R., Ueberberg H. 1978; Ultrastructural investigation of the formation of Candida albicans germ tubes and septa. Sabouraudia 16:115–124
    [Google Scholar]
  19. Stanley V. C., Hurley R. 1969; The growth of Candida species in cultures of mouse peritoneal macrophages. Journal of Pathology 97:357–366
    [Google Scholar]
  20. Wilson G. S., Miles A. A. 1964 Topley and Wilson’s Principles of Bacteriology and Immunity 1 p. 266 London:: Edward Arnold;
    [Google Scholar]
  21. Yamamura M., Valdimarsson H. 1977; Participation of C3in intracellular killing of Candida albicans. Scandinavian Journal of Immunology 6:591–594
    [Google Scholar]
  22. Yamamura M., Boler J., Valdimarsson H. 1977; Phagocytosis measured as inhibition of uridine uptake by Candida albicans. Journal of Immunological Methods 14:19–24
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-3-465
Loading
/content/journal/micro/10.1099/00221287-130-3-465
Loading

Data & Media loading...

Most cited Most Cited RSS feed