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Lipopolysaccharide and protein material were released from cells of Desulfovibrio vulgaris on treatment with 10 mm-EDTA. Preincubation with up to 15 mm-ferrous ions caused increased removal of lipopolysaccharide from bacteria grown in iron-limited medium, but preincubation with calcium, magnesium, or zinc ions was unable to induce this response. Ferrous ions appear to have an important role in the stabilization in vivo of the outer membrane of D. vulgaris. The selective interaction between ferrous ions and D. vulgaris lipopolysaccharide may play a part in iron uptake.
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