1887

Abstract

Lipopolysaccharide and protein material were released from cells of on treatment with 10 m-EDTA. Preincubation with up to 15 m-ferrous ions caused increased removal of lipopolysaccharide from bacteria grown in iron-limited medium, but preincubation with calcium, magnesium, or zinc ions was unable to induce this response. Ferrous ions appear to have an important role in the stabilization of the outer membrane of The selective interaction between ferrous ions and lipopolysaccharide may play a part in iron uptake.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-2-441
1984-02-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/2/mic-130-2-441.html?itemId=/content/journal/micro/10.1099/00221287-130-2-441&mimeType=html&fmt=ahah

References

  1. Galanos C., LÜderitz O., Rietschel E. T., Westphal O. 1977; New aspects of the chemistry and biology of bacterial lipopolysaccharides, with special reference to their lipid A component. International Review of Biochemistry 14:239–335
    [Google Scholar]
  2. Hancock R. E. W. 1981; Aminoglycoside uptake and mode of action. Journal of Antimicrobial Chemotherapy 8:429–445
    [Google Scholar]
  3. Iverson W. P. 1974; Microbial corrosion of iron. In Microbial Iron Metabolism pp. 475–513 Neilands J. B. Edited by New York:: Academic Press;
    [Google Scholar]
  4. Iverson W. P., Olson G. J. 1983; Anaerobic corrosion by sulfate-reducing bacteria due to a highly reactive volatile phosphorous compound. In Microbial Corrosion Proceedings of a conference at the National Physical Laboratory, Teddington March 1983 pp. 46–53 London:: The Metals Society;
    [Google Scholar]
  5. Konisky J. 1979; Specific transport systems and receptors for colicins and phages. In The Bacterial Outer Membrane: Biogenesis and Functions pp. 319–332 Inouye M. Edited by New York:: Wiley;
    [Google Scholar]
  6. Kropinski A. M., Kuzio J., Angus B. L., Hancock R. E. W. 1982; Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic super-susceptible mutant of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy 21:310–319
    [Google Scholar]
  7. Leive L. 1974; The barrier function of the Gramnegative cell envelope. Armais of the New York Academy of Sciences 235:109–129
    [Google Scholar]
  8. Leive L., Shoucin V. K., Mergenhagen S. E. 1968; Physiological, chemical and immunological properties of lipopolysaccharide released from E. coli by EDTA. Journal of Biological Chemistry 243:6384–6391
    [Google Scholar]
  9. Mcintosh M. A., Earhart C. F. 1976; Effect of iron on the relative abundance of two large polypeptides of the E. coli outer membrane. Biochemical and Biophysical Research Communications 70:315–322
    [Google Scholar]
  10. Meyer J. M., Mock M., Abdallah M. A. 1979; Effect of iron on the protein composition of the outer membrane of a fluorescent pseudomonad. FEMS Microbiology Letters 5:395–398
    [Google Scholar]
  11. Miller J. D. A. 1971 Microbial Aspects of Metallurgy Aylesbury:: Medical & Technical Publishing Co.;
    [Google Scholar]
  12. Morrissey J. H. 1981; Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117:307–310
    [Google Scholar]
  13. Neilands J. B. (editor) 1974; Iron and its role in microbial physiology. In Microbial Iron Metabolism pp. 4–34 New York:: Academic Press;
    [Google Scholar]
  14. Nicas T. J., Hancock R. E. W. 1983; Alteration of the susceptibility to EDTA, polymyxin B and genta- mycin in Pseudomonas aeruginosa by divalent cation regulation of the outer membrane protein HI. Journal of General Microbiology 129:509–517
    [Google Scholar]
  15. Postgate J. R. 1981 The Sulphale-reducing Bacteria Cambridge:: Cambridge University Press;
    [Google Scholar]
  16. Schindler M., Osborn M. J. 1979; Interaction of divalent cations and polymyxin B with lipopoly-saccharide. Biochemistry 18:4425–4430
    [Google Scholar]
  17. Westphal O., Jann K. 1965; Bacterial lipopolysaccharide.Extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  18. Zey P., Jackson S. 1973; Conditions that affect the colorimetric analysis of lipopolysaccharides from E. coli and Treponemapallidum. Applied Microbiology 26:129–133
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-2-441
Loading
/content/journal/micro/10.1099/00221287-130-2-441
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error