1887

Abstract

The site selection for bud and germ tube emergence has been studied in the dimorphic yeast The fluorescent dye Calcofluor was used to stain bud scars. Two different modes of site selection were observed in yeasts. On cells cultivated at 23–28 °C, buds emerged primarily at one end of the cell at adjacent sites suggesting a type of persistent memory. Sites selected on yeasts growing at 37 °C were non-adjacent and scattered over the cell. The pattern of bud emergence during resumption of budding from stationary phase, or after temperature shift between conditions, was not a simple transition between modes. During these transitions the budding pattern sequentially passed through non-adjacent and adjacent budding modes before establishing the pattern characteristic of the growth temperature. Germ tubes emerged in a non-adjacent pattern from cells grown to stationary phase at 28 °C. The higher frequency of non-adjacent emergence and the absence of sequential pattern changing in site selection for germ tubes suggested that non-adjacent site selection for germ tubes and buds might not be the same.

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/content/journal/micro/10.1099/00221287-130-2-431
1984-02-01
2021-10-19
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References

  1. Beran K. 1968; Budding of yeast cells, their scars and ageing. Advances in Microbial Physiology 2:143–171
    [Google Scholar]
  2. Cassone A., Simonetti N., Strippoli V. 1973; Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans . Journal of General Microbiology 77:417–426
    [Google Scholar]
  3. Chaffin W. L., Sogin S. J. 1976; Germ tube formation from zonal rotor fractions of Candida albicans . Journal of Bacteriology 126:771–776
    [Google Scholar]
  4. Chattaway F. W., Holmes M. R., Barlow A. J. E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans . Journal of General Microbiology 51:367–376
    [Google Scholar]
  5. Chattaway F. W., Bishop R., Holmes M. R., Odds F. C. 1973; Enzyme activities associated with carbohydrate synthesis and breakdown in the yeast and mycelial forms of Candida albicans . Journal of General Microbiology 75:97–109
    [Google Scholar]
  6. Farkaš V. 1979; Biosynthesis of cell walls of fungi. Microbiological Reviews 43:117–144
    [Google Scholar]
  7. Hartwell L. H., Unger M. W. 1977; Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. Journal of Cell Biology 75:422–435
    [Google Scholar]
  8. Hayashibe M., Katohda S. 1973; Initiation of budding and chitin ring. Journal of General and Applied Microbiology 19:23–39
    [Google Scholar]
  9. Jacobs C. W. 1983 Events associated with cellular development and differentiation in Wangiella dermati- tidis Ph.D thesis University of Texas at Austin, USA:
    [Google Scholar]
  10. Lee K. L., Buckley H. R. 1975; An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans . Sabouraudia 13:148–153
    [Google Scholar]
  11. Mackenzie D. W. R. 1964; Morphogenesis of Candida albicans in vivo . Sabouraudia 3:225–232
    [Google Scholar]
  12. Maeda H., Ishida N. 1967; Specificity of binding hexopyranosyl polysaccharides with fluorescent brightener. Journal of Biochemistry 62:276–278
    [Google Scholar]
  13. Mitchell L. H., Soll D. R. 1979a; Commitment to germ tube or bud formation during release from stationary phase in Candida albicans . Experimental Cell Research 120:167–179
    [Google Scholar]
  14. Mitchell L., Soll D. R. 1979b; Temporal and spatial differences in septation during synchronous mycelium and bud formation in Candida albicans . Experimental Mycology 3:298–309
    [Google Scholar]
  15. Nickerson W. J. 1963; Symposium on biochemical bases of morphogenesis in fungi. IV. Molecular bases of form in yeasts. Bacteriological Reviews 27:305–324
    [Google Scholar]
  16. Roberts R. L., Szaniszlo P. J. 1978; Temperature-sensitive multicellular mutants of Wangiella dermatitidis . Journal of Bacteriology 135:622–632
    [Google Scholar]
  17. Roberts R. L., Szaniszlo P. J. 1980; Yeast-phase cell cycle of the polymorphic fungus Wangiella dermatitidis . Journal of Bacteriology 144:721–731
    [Google Scholar]
  18. Roberts R. L., Lo R. J., Szaniszlo P. J. 1979; Nuclear division in temperature-sensitive multicellular mutants of Wangiella dermatitidis . Journal of Bacteriology 137:1456–1458
    [Google Scholar]
  19. Sloat B. F., Adams A., Pringle J. R. 1981; Roles of the CDC24 gene product in cellular morphogenesis during the Saccharomyces cerevisiae cell cycle. Journal of Cell Biology 89:395–405
    [Google Scholar]
  20. Soll D. R., Bedell G. W. 1978; Bud formation and the inducibility of pseudo-mycelium outgrowth during release from stationary phase in Candida albicans . Journal of General Microbiology 108:173–180
    [Google Scholar]
  21. Soll D. R., Mitchell L. H. 1983; Filament ring formation in the dimorphic yeast Candida albicans . Journal of Cell Biology 96:486–493
    [Google Scholar]
  22. Szaniszlo P. J., Hseih P. H., Marlowe J. D. 1976; Induction and ultrastructure of the multicellular (sclerotic) morphology in Phialophora dermatiti-dis . Mycologia 68:117–130
    [Google Scholar]
  23. Thompson P. W., Wheals A. E. 1980; Asymmetrical division of Saccharomyces cerevisiae in glucose-limited chemostat culture. Journal of General Micro-biology 121:401–409
    [Google Scholar]
  24. Wain W. H., Price M. F., Brayton A. R., Cawson R. A. 1976; Macromolecular synthesis during the cell cycles of the yeast and hyphal phases of Candida albicans . Journal of General Microbiology 97:211–217
    [Google Scholar]
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