1887

Abstract

Microdensitometry showed that the membrane profiles of normal cultivable mycobacteria were very asymmetric (outer layer denser and thicker than the inner layer), while the profiles of normal-looking in lepromatous patients, in experimentally infected armadillos and in nude mice were approximately symmetric; moreover, the membrane of was thicker than that of cultivable species. Using two cytochemical methods for the ultrastructural detection of periodic acid-Schiff (PAS)-positive molecules (the Thiry procedure, and staining with phosphotungstic acid at low pH) we found that the membrane of cultivable mycobacteria, growing or , had PAS-positive components exclusively in the outer layer, while the normal-looking in patients and in armadillos had membranes with PAS-positive components in both layers. The membranes of damaged cultivable mycobacteria, or , and of damaged , in patients or armadillos, were PAS-negative.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-2-369
1984-02-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/2/mic-130-2-369.html?itemId=/content/journal/micro/10.1099/00221287-130-2-369&mimeType=html&fmt=ahah

References

  1. Cohn Z., Benson B. 1965; The differentiation of mononuclear phagocytes. Journal of Experimental Medicine 121:153–169
    [Google Scholar]
  2. Colston M. J., Hilson G.R.S. 1976; Growth ofMycobacterium leprae andM. marinum in congenitally athymic (nude) mice. Nature, London 262:399–401
    [Google Scholar]
  3. Fléchon J.-E., Huneau D. 1974; Validity of phosphotungstic acid staining of polysaccharides (glycogen) at very low pH on thin sections of glycol methacrylate embedded material. Journal de microscopie 19:207–212
    [Google Scholar]
  4. Jopling W. H. 1978 Handbook of Leprosy p. 9 London: Heinemann;
    [Google Scholar]
  5. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409–414
    [Google Scholar]
  6. Petitprez A., Derieux J. C. 1970; Mise en evidence des polysaccharides sur quelque types de bacteries. Journal de microscopie 9:263–272
    [Google Scholar]
  7. Portaels F., Pattyn S. R. 1981; Parameters influencing thein vitro growth ofM. lepraemurium. . International Journal of Leprosy 49:194–197
    [Google Scholar]
  8. Portaels F., Pattyn S. R. 1982; Isolation of fastidiously growingMycobacteria from armadillo livers infected withMycobacterium leprae. . International Journal of Leprosy 50:370–374
    [Google Scholar]
  9. Rambourg A. 1971; Morphological and histochemi- cal aspects of glycoproteins of the surface of animal cells. International Review of Cytology 31:57–114
    [Google Scholar]
  10. Ridley D. S., Jopling W. H. 1964; Classification of leprosy according to immunity. A five group system. International Journal of Leprosy 34:255–273
    [Google Scholar]
  11. Robertson J. G., Lyttleton P., Williamson K. I., Batt R. D. 1975; The effects of fixation procedures on the electron density of polysaccharide granules inNocardia corallina. . Journal of Ultrastructure Research 52:321–332
    [Google Scholar]
  12. Roland J. C. 1974; Cytochimie des polysaccharides vegetaux: detection et extraction selectives. Journal de microscopie 21:233–244
    [Google Scholar]
  13. Rosseau M., Hermier J. 1975; Localization en microscopie électronique des polysaccharides de la paroi chez les bacteries en sporulation. Journal de microscopie 23:237–248
    [Google Scholar]
  14. Silva M. T. 1967; Electron microscopic aspects of membrane alterations during bacterial cell lysis. Experimental Cell Research 46:245–251
    [Google Scholar]
  15. Silva M. T. 1971; Changes induced in the ultrastructure of the cytoplasmic and intracytoplasmic membranes of several Gram-positive bacteria by variations in Os04 fixation. Journal of Microscopy 93:227–232
    [Google Scholar]
  16. Silva M. T. 1975; Ultrastructure of the membranes of Gram-positive bacteria as influenced by fixatives and membrane-damaging treatments. In Biomembranes - Lipids, Proteins and Receptors pp. 255–289 Edited by Burton R. M., Packer L. Webster Groves, Mo., USA: Bl-Science Publication Division;
    [Google Scholar]
  17. Silva M. T. 1984; The use of transmission electron microscopy of ultrathin sections for the characterization of the ultrastructure of normal and damaged bacterial membranes. In Biomembranes: Dynamics and Biology Edited by Guerra F. C., Burton R. M. New York: Plenum Press (in the Press);
    [Google Scholar]
  18. Silva M. T., Macedo P. M. 1982a; Ultrastructure ofMycobacterium leprae and other acid-fast bacteria as influenced by fixation conditions. Annales de microbiologie 133B:59–73
    [Google Scholar]
  19. Silva M. T., Macedo P. M. 1982b; Comparative ultrastructural and cytochemical study of the membranes of Mycobacterium leprae and cultivable Mycobacteria. . XVII Reunião Anual, Sociedade Portuguesa Microscopia Electronica abs. 26
    [Google Scholar]
  20. Silva M. T., Macedo P. M. 1983a; Electron microscopic study ofMycobacterium leprae membrane. International Journal of Leprosy 51:219–224
    [Google Scholar]
  21. Silva M. T., Macedo P. M. 1983b; The interpretation of the ultrastructure ofMycobacteriumcells in transmission electron microscopy of ultrathin sections. International Journal of Leprosy 51:225–234
    [Google Scholar]
  22. Silva M. T., Macedo P. M. 1983c; A comparative ultrastructural study of the membranes of Mycobacterium leprae and of cultivable Mycobacteria. . Biology of the Cell 41:383–386
    [Google Scholar]
  23. Silva M. T., Sousa J.C.F. 1972; Ultrastructural alterations induced by moist heat inBacillus cereus. . Applied Microbiology 24:463–476
    [Google Scholar]
  24. Silva M. T., Sousa J.C.F., Macedo M.A.F., Polónia J., Parente A. M. 1976; Effects of phenethyl alcohol inBacillus andStreptococcus. . Journal of Bacteriology 127:1359–1369
    [Google Scholar]
  25. Silva M. T., Polónia J. J., Kocur M. 1977; The fine structure ofMicrococcus mucilaginosus. . Journal of Submicroscopic Cytology 9:53–66
    [Google Scholar]
  26. Silva M. T., Sousa J.C.F., Balassa G. 1978; Ultrastructural effects of chemical agents and moist heat onBacillus subtilis. I. Effects on vegetative cells. Annales de microbiologie 129B:363–375
    [Google Scholar]
  27. Silva M. T., Polónia J. J., Macedo M.A.E., Macedo P. M. 1979a; Membrane splitting induced by lipophilic molecules. Biologie cellulaire 35:175–182
    [Google Scholar]
  28. Silva M. T., Sousa J.C.F., Polónia J. J., Macedo P. M. 1979b; Effects of local anestheticson bacterial cells. Journal of Bacteriology 137:461–468
    [Google Scholar]
  29. Silva M. T., Macedo P. M., Costa M.H.L., Gonsalves H., Torgal J., David H. L. 1982; a)Ultrastructural alterations ofMycobacterium lepraein skin biopsies of untreated and treated leproma- tous patients. Annales de microbiologie 133B:75–92
    [Google Scholar]
  30. Silva M. T., Macedo P. M., Salema R., Santos I. 1982b; Improvement in the Thiery reaction by the use of silver vitelinate. XVII Reunião Anual, Sociedade Portuguesa Microscopia Electronica abs. 30
    [Google Scholar]
  31. Thiéry J. P. 1967; Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. Journal de microscopie 6:987–1018
    [Google Scholar]
  32. Thiéry J. P., Rambourg A. 1974; Cytochimie des polysaccharides. Journal de microscopic 21:225–232
    [Google Scholar]
  33. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25:407–408
    [Google Scholar]
  34. Wicken A. J., Knox K. W. 1980; Bacterial cell surface amphiphiles. Biochimica et biophysica acta 604:1–26
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-2-369
Loading
/content/journal/micro/10.1099/00221287-130-2-369
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error