RT Journal Article SR Electronic(1) A1 Gopal, Pramod A1 Sullivan, Patrick A. A1 Shepherd, Maxwell G.YR 1984 T1 Metabolism of [14C]Glucose by Regenerating Spheroplasts of Candida albicans JF Microbiology, VO 130 IS 2 SP 325 OP 335 DO https://doi.org/10.1099/00221287-130-2-325 PB Microbiology Society, SN 1465-2080, AB Spheroplasts of Candida albicans were regenerated in [14C]glucose and buffered magnesium sulphate (0·1 m-Tris/HC1; 0·5 m-MgSO4, pH 7.2) at 35C. Uptake of glucose by spheroplasts was faster than that by intact yeast cells. After 6 h, 65% of the glucose taken up by the yeast appeared as CO2 and 30% was incorporated into the cellular material. With spheroplasts, 55% of the glucose taken up was expired as CO2, 25% was excreted into the medium as other metabolites and 20% was incorporated into the cells. The regenerating spheroplasts excreted 14C-labelled carbohydrates into the medium which were fractionated on a Sephadex G-15 column. Acid hydrolysis of the low molecular-weight fraction yielded the following sugars: mannose (75·7%), fucose (3·8%), arabinose (3%), galactose (2·1%) and an unidentified monosaccharide (14%). Spheroplasts did not incorporate mannoprotein into the regenerated wall. The wall carbohydrate from regenerated spheroplasts was fractionated on the basis of solubility in sodium hydroxide. The alkali-insoluble fraction was analysed by sequential enzyme hydrolysis; 40% of the incorporated counts were associated with β(1 → 3)-linked glucan and 50% with a mixed glucan comprising β(1 → 3)- and β(1 → 6)-linkages and chitin., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-2-325