1887

Abstract

Preliminary studies demonstrated that synthesized isoleucine by a pathway not involving threonine or threonine dehydratase. Radiotracer experiments with cells grown in a defined carbohydrate-free medium showed that radioactivity from [UC]serine, [3C]pyruvate, [C]NaHCO and [1-], [2-] and [3C]propionate was incorporated into isoleucine. Conversely, there was no detectable incorporation of C into isoleucine during growth with [UC]glutamate, [UC]threonine, [UC]valine, [UC]leucne or [UC]methionine. Crude extracts of the bacteria grown in a minimal medium contained levels of a-acetohydroxyacid synthase activities comparable to those in K12 grown in minimal medium. Stepwise degradation of isoleucine obtained from grown in the presence of specifically-labelled precursors indicated that can make isoleucine via the reductive carboxylation of propionate to yield a-oxobutyrate, which is metabolized to isoleucine in the classical fashion. Isoleucine was also formed by via the reductive carboxylation of a-methylbutyrate to α-oxo--methylvalerate.

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1984-02-01
2021-10-19
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References

  1. Allison M. J., Bryant M. P. 1963; Biosynthesis of branched-chain amino acids from branched-chain fatty acids by rumen bacteria. Archives of Biochemistry and Biophysics 101:269–277
    [Google Scholar]
  2. Allison M. J., Robinson I. M. 1970; Biosynthesis of a-ketoglutarate by the reductive carboxylation of succinate inBacteroides ruminicola.. Journal of Bacteriology 104:50–56
    [Google Scholar]
  3. Allison M. J., Robinson I. M., Baetz A. L. 1979; Synthesis of α-ketoglutarate by reductive carboxylation of succinate inVeillonella,Selenomonas andBacteroides species. Journal of Bacteriology 140:980–986
    [Google Scholar]
  4. Barker H. A. 1981; Amino acid degradation by anaerobic bacteria. Annual Review of Biochemistry 50:23–40
    [Google Scholar]
  5. Buchanan B. B. 1969; Role of ferredoxin in the synthesis of α-ketobutyrate from propionyl coenzyme A and carbon dioxide by enzymes from photosynthetic and non-photosynthetic bacteria. Journal of Biological Chemistry 244:4218–4223
    [Google Scholar]
  6. Buchanan B. B. 1972; Ferredoxin-linked carboxylation reactions. In The Enzymes VI pp. 193–216 Edited by Boyer P. D. New York: Academic Press;
    [Google Scholar]
  7. Buchanan B. B., Arnon D. I. 1969; The reductive carboxylic acid cycle. Methods in Enzymology 13:170–181
    [Google Scholar]
  8. Buchanan B. B., Evans M.C.W. 1965; The synthesis of α-ketoglutarate from succinate and carbon dioxide by a subcellular preparation of a photosynthetic bacterium. Proceedings of the National Academy of Sciences of the United States of America 54:1212–1218
    [Google Scholar]
  9. Bush R. S., Sauer F. D. 1976; Enzymes of 2-oxo acid degradation and biosynthesis in cell-free extracts of mixed rumen microorganisms. Biochemical Journal 157:325–331
    [Google Scholar]
  10. Campbell L. L.Jr Frank H. A. 1956; Nutritional requirements of some putrefactive anaerobic bacteria. Journal of Bacteriology 71:267–269
    [Google Scholar]
  11. Charon N. W., Johnson R. C., Peterson D. 1974; Amino acid biosynthesis in the spirocheteLeptospira: evidence for a novel pathway of isoleucine synthesis. Journal of Bacteriology 117:203–211
    [Google Scholar]
  12. Costilow R. N. 1977; Selenium requirement for growth of Clostridium sporogenes with glycine as the oxidant in Stickland reaction systems. Journal of Bacteriology 131:366–368
    [Google Scholar]
  13. Davis B. D., Mingoli E. S. 1950; Mutants ofEscherichia coli requiring methionine or vitamin B,2. Journal of Bacteriology 60:17–28
    [Google Scholar]
  14. Gladstone G. P. 1939; Interrelationships between amino-acids in the nutrition of Bacillus anthracis.. British Journal of Experimental Pathology 20:189–200
    [Google Scholar]
  15. Greenberg D. M., Rothstein M. 1957; Methods for chemical synthesis, isolation and degradation of labeled compounds as applied in metabolic studies of amino acids and proteins. Methods in Enzymology 4:652–731
    [Google Scholar]
  16. Grimminger H., Umbarger H. E. 1979; Acetohydroxy acid synthaselof Escherichiacoli : purification and properties. Journal of Bacteriology 137:846–853
    [Google Scholar]
  17. Hadioetomo R. S. 1980 Amino acid biosynthesis in Clostridium sporogenes: evidence for a novel pathway for isoleucine biosynthesis Ph.D. thesis Michigan State University: USA;
    [Google Scholar]
  18. Iaccarino M., Guardiola J., Defelice M., Fauro R. 1978; Regulation of isoleucine and valine biosynthesis. Current Topics in Cellular Regulation 14:29–73
    [Google Scholar]
  19. Kemble A. R., Mcpherson H. T. 1954; Determination of monoamino monocarboxylic acids by quantitative paper chromatography. Journal of Biochemistry 56:548–555
    [Google Scholar]
  20. Kindler S. H., Mager J. 1956; Nutritional studies with theClostridium botulinum group. Journal of General Microbiology 15:386–393
    [Google Scholar]
  21. Lee W. H., Riemann H. 1970; The genetic relatedness of proteolyticClostridium botulinumstrains. Journal of General Microbiology 64:85–90
    [Google Scholar]
  22. Mcbride R. W., Jolly D. W., Kadis B. M., Nelson T. E.Jr 1979; Rapid thin-layer chromatographic separation of isoleucine, leucine and phenylalanine. Chromatography 11:424–425
    [Google Scholar]
  23. Mager J., Kindler S. H., Grossowicz N. 1954; Nutritional studies withClostridium parabotulinum type A. Journal of General Microbiology 10:130–141
    [Google Scholar]
  24. Mead G. C. 1971; The amino acid-fermenting Clostridia. Journal of General Microbiology 67:47–56
    [Google Scholar]
  25. Monticello D. J., Costilow R. N. 1982; Interconversion of valine and leucine byClostridium sporogenes.. Journal of Bacteriology 152:946–949
    [Google Scholar]
  26. Perkins W. E., Tsuji K. 1962; Sporulation ofClostridium botulinum. II. Effect of arginine and its degradation products on sporulation in a synthetic medium. Journal of Bacteriology 84:86–94
    [Google Scholar]
  27. Phares E. F. 1951; Degradation of labeled propionic and acetic acids. Analytical Chemistry 23:168–174
    [Google Scholar]
  28. Phillips A. T., Nuss J. I., Moosic J., Foshay C. 1972; Alternate pathway for isoleucine biosynthesis inEscherichia coli.. Journal of Bacteriology 109:714–719
    [Google Scholar]
  29. Robinson I. M., Allison M. J. 1969; Isoleucine biosynthesis from 2-methylbutyric acid by anaerobic bacteria from the rumen. Journal of Bacteriology 97:1220–1226
    [Google Scholar]
  30. Sauer F. D., Erfle J. D., Mahadevan S. 1975; Amino acid biosynthesis in mixed rumen cultures. Biochemical Journal 150:357–372
    [Google Scholar]
  31. Schildkraut I., Greer S. 1973; Threonine synthase-catalyzed conversion of phosphohomoserine to α-ketobutyrate inBacillus subtilis.. Journal of Bacteriology 115:777–785
    [Google Scholar]
  32. Størmer F. C., Umbarger H. E. 1964; The requirement for flavine adenine dinucleotide in the formation of acetolactate bySalmonella typhimuriumextracts. Biochemical and Biophysical Research Com-munications 17:587–592
    [Google Scholar]
  33. Strassman M., Thomas A. J., Locke L. A., Weinhouse S. 1956; The biosynthesis of isoleucine. Journal of the American Chemical Society 78:228–232
    [Google Scholar]
  34. Umbarger H. E. 1973; Threonine deaminases. Advances in Enzymology 37:349–395
    [Google Scholar]
  35. Umbarger H. E. 1978; Amino acid biosynthesis and its regulation. Annual Review of Biochemistry 47:533–606
    [Google Scholar]
  36. Whitely H. R., Tahara M. 1966; Threonine deaminase ofClostridium tetanomorphum. I. Purification and properties. Journal of Biological Chemistry 241:4881–4889
    [Google Scholar]
  37. Wu J.I.J., Riemann H., Lee W. H. 1972; Thermal stability of the deoxyribonucleic acid hybrids between the proteolytic strains ofClostridium botulinum andClostridium sporogenes.. Canadian Journal of Microbiology 18:97–99
    [Google Scholar]
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