1887

Abstract

A lytic enzyme was isolated and purified from PL-1 phage-induced lysates of the host ATCC 27092. The molecular weight of the enzyme was about 30000. Maximum activity on the lysis of the host cell walls occurred at pH 6.0-6.5 and at 45 °C. The enzyme activity was inhibited by heavy metal ions, SH- and serine-enzyme inhibitors and -phenanthroline. The reducing end of the enzymic digest was muramic acid and the enzyme was considered to be an endo--acetylmuramidase. However, the enzyme differed from the other known -acetylmuramidases including hen's egg-white lysozyme in several enzymic properties.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-2-275
1984-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/2/mic-130-2-275.html?itemId=/content/journal/micro/10.1099/00221287-130-2-275&mimeType=html&fmt=ahah

References

  1. Imoto T., Yagishita K. 1971; A simple activity measurement of lysozyme. Agricultural and Biological Chemistry 35:1154–1156
    [Google Scholar]
  2. Imoto T., Johnson L. N., North A.C.T., Phillips D. C., Rupley J. A. 1972; Vertebrate lysozymes. In The Enzymes, 3rd edn. VII pp. 665–868 Edited by Boyer P. D. New York & London: Academic Press;
    [Google Scholar]
  3. Ishibashi K., Takesue S., Watanabe , Oishi K. 1982; Use of lectins to characterize the receptor sites for bacteriophage PL-1 of Lactobacillus casei. Journal of General Microbiology 128:2251–2259
    [Google Scholar]
  4. Rogers H. J. 1979; The function of bacterial auto- lysins. In Microbial Polysaccharides and Polysaccharases pp. 237–268 Edited by Berkeley R. C. W., Gooday G. W., Ellwood D. C. London, New York & San Francisco: Academic Press;
    [Google Scholar]
  5. Strominger J. L., Ghuysen J. M. 1967; Mechanism of enzymatic bacteriolysis. Science 156:213–221
    [Google Scholar]
  6. Tsugita A., Inouye M., Terzaghi E., Strei-Singer G. 1968; Purification of bacteriophage T4 lysozyme. Journal of Biological Chemistry 243:391–397
    [Google Scholar]
  7. Tsuru D. 1977; History and present state of the research on lytic enzymes.(Japanese). In Yohkin- Kohso pp. 1–13 Edited by Funatsu M., Tsuru D. Tokyo: Kohdansha Co;
    [Google Scholar]
  8. Tsuru D. 1983; Microbial cell wall-lytic enzymes.(Japanese). Hakko to Kogyo 41:180–193
    [Google Scholar]
  9. Watanabe K., Takesue S., Jin-Nai K., Yoshikawa T. 1970; Bacteriophage active against the lactic acid beverage-producing bacterium Lactobacillus casei. Applied Microbiology 20:409–415
    [Google Scholar]
  10. Watanabe K., Takesue S., Ishibashi K. 1979; Adenosine triphosphate content in Lactobacillus caseiand the blender-resistant phage-cell complexforming ability of cells on infection with PL-1 phage. Journal of General Virology 42:27–36
    [Google Scholar]
  11. Yemm E. W., Cocking E. C. 1955; The determination of amino acids with ninhydrin. Analyst 80:209–213
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-2-275
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error