@article{mbs:/content/journal/micro/10.1099/00221287-130-2-223, author = "Marounek, M. and Wallace, R. J.", title = "Influence of Culture Eh on the Growth and Metabolism of the Rumen Bacteria Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and Streptococcus bovis in Batch Culture", journal= "Microbiology", year = "1984", volume = "130", number = "2", pages = "223-229", doi = "https://doi.org/10.1099/00221287-130-2-223", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-2-223", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "One facultatively and three strictly anaerobic rumen bacteria were grown in pH-controlled anaerobic batch cultures in which the E h of the medium was regulated by the addition of titanium (III) citrate solution at values below −50 mV and potassium ferricyanide above −50 mV. Growth occurred over a wide range of E h, with the maximum limit being +360, + 250, +175 and +414 mV for Selenomonas ruminantium, Bacteroides amylophilus, Bacteroides succinogenes and the aerotolerant Streptococcus bovis, respectively. Changes in E h had little influence on the growth yield or ratios of fermentation end-products in these bacteria over a fairly wide range, although the specific growth rate of all species tended to decline at E h values above 0 mV. Abnormal, elongated forms of Sel. ruminantium and B. succinogenes predominated at high E h. It was concluded that O2, and not a high E h, is the toxic factor in ‘oxidized’ anaerobic growth medium, and that it would not normally be necessary to regulate E h closely in experiments where the growth and metabolism of these bacteria is under study, provided that O2-free conditions were maintained.", }