Candida tropicalis Pk 233 produced extracellular glutathione during growth in filamentous form caused by adding ethanol (⩾ 2·5%, v/v). The intracellular concentration of glutathione also was greater in cultures with added ethanol. myo-Inositol added at a physiological concentration (5 µg ml−1) prevented the ethanol-induced production of extracellular glutathione as well as the morphological change. In batch culture, production of extracellular glutathione was optimal at an ethanol concentration of 5%, and reached a maximum concentration of 42 mg ml−1 after 96 h cultivation, before decreasing gradually.
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