1887

Abstract

The mechanism of resistance to the arginine analogue -canavanine, and of arginine uptake, were examined in the fission yeast Two mutants with increased resistance to canavanine were analysed genetically: both were double mutants, and in each case one mutation conferred resistance to canavanine, while the other enhanced this resistance. Evidence is presented that strains are defective in one system for arginine uptake, which presumably prevents entry of canavanine into the cell. This system operates in the wild-type whether the nitrogen source supplied is ammonium or glutamate. Double mutants carrying and an arginine requirement are unable to grow on ammonium medium even when supplied with exogenous argine, while growth can occur on glutamate plus arginine. This suggested the existence of a second uptake system for arginine which is absent during growth on ammonium, and direct measurements of the rates of arginine uptake under various conditions confirmed this. Our observations closely parallel those made on the budding yeast The ability to select for or against function of the gene should facilitate certain types of genetical analysis in

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-12-3265
1984-12-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/12/mic-130-12-3265.html?itemId=/content/journal/micro/10.1099/00221287-130-12-3265&mimeType=html&fmt=ahah

References

  1. Beach D., Nurse P. 1981; High frequency transformation of the fission yeast Schizosaccharomyces pombe. . Nature; London: 290140–142
    [Google Scholar]
  2. Cooper T.G. 1982; Transport in Saccharomyces cerevisiae. . In The Molecular Biology of the Yeast Saccharomyces II pp. 399–461 Edited by Strathern J., Jones E., Broach J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  3. Creanor J., Mitchison J.M. 1982; Patterns of protein synthesis during the cell cycle of the fission yeast Schizosaccharomyces pombe. . Journal of Cell Science 58:263–285
    [Google Scholar]
  4. Grenson M., Hennaut C. 1971; Mutation affecting activity of several distinct amino acid transport systems in Saccharomyces cerevisiae. . Journal of Bacteriology 105:477–482
    [Google Scholar]
  5. Grenson M., Hou C. 1972; Ammonia inhibition of the general amino acid permease and its suppression in the NADPH-specific glutamate dehydrogenaseless mutants of Saccharomyces cerevisiae. . Biochemical and Biophysical Research Communications 48:749–756
    [Google Scholar]
  6. Grenson M., Mousset M., Wiame J.-M., Bechet J. 1966; Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system. Biochimica et biophysica acta 127:325–338
    [Google Scholar]
  7. Grenson M., Hou C., Crabeel M. 1970; Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease. Journal of Bacteriology 103:770–777
    [Google Scholar]
  8. Gutz H., Heslot H., Leupold U., Loprieno N. 1974; Schizosaccharomyces pombe. In Handbook of Genetics pp. 395–446 Edited by King R. C. New York: Plenum Press;
    [Google Scholar]
  9. Gygax A., Thuriaux P. 1984; A revised chromosome map of the fission yeast Schizosaccharomyces pombe. . Current Genetics 8:85–92
    [Google Scholar]
  10. Johnston P.A., Coddington A. 1983; Multiple drug resistance in the fission yeast Schizosaccharomyces pombe: correlation between drug and amino acid uptake and membrane ATPase activities. Current Genetics 7:299–307
    [Google Scholar]
  11. Kohli J., Hottinger H., Munz P., Strauss A., Thuriaux P. 1977; Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic analysis and induced haploidization. Genetics 87:471–489
    [Google Scholar]
  12. Mitchison J.M. 1970; Physiological and cytological methods for Schizosaccharomyces pombe. . Methods in Cell Physiology 4:131–165
    [Google Scholar]
  13. Strauss A. 1979; Isolation and genetical classifica-tion of aromatic amino acid auxotrophic mutants in Schizosaccharomyces pombe. . Journal of General Microbiology 113:173–176
    [Google Scholar]
  14. Surdin Y., Sly W., Sire J., Bordes A.M., Derobichon-Szulmajster H. 1965; Propriétés et contrôle génétique du systéme d’accumulation des acides amines chez Saccharomyces cerevisiae. . Biochimica et biophysica acta 107:546–566
    [Google Scholar]
  15. Whelan W.L., Gocke E., Manney T.R. 1979; The CAN 1 locus of Saccharomyces cerevisiae: fine structure analysis and forward mutation rates. Genetics 91:35–51
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-12-3265
Loading
/content/journal/micro/10.1099/00221287-130-12-3265
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error