Some Biochemical and Morphological Characteristics of Serotypes of from Hop Free

Abstract

Single-spore strains of from hop, which had previously been classified into three serotypes by immunoelectrophoretic and immunofluorescence methods, have been further characterized using a series of morphological and biochemical parameters. Serotype 1 strains were characterized by high sporulation and low polygalacturonase activity when cultured on hop root cell walls, the presence of certain protein bands on isoelectric focusing of mycelial extracts, and a characteristic dark-pigmented mycelial morphology on prune/lactose/yeast extract agar. It is suggested that the expression of serotype 1 characteristics is controlled by the previously reported hyl cytoplasmic factor. Serotypes 2 and 3 were both associated with low sporulation and high polygalacturonase activity and a fully hyaline fluffy mycelium, although many serotype 3 strains were only partially hyaline. Serotype 2 was associated with a characteristic pattern of mycelial proteins on isoelectric focusing gels: serotype 3 strains showed a heterogeneous series of patterns. None of the characters studied could be correlated with virulence for hop but the results do allow the selection for future studies of well-characterized strains which are extremely similar in many respects and yet still differ markedly in virulence.

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1984-12-01
2024-03-28
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References

  1. Bell A.A. 1969; Phytoalexin production and Verticillium resistance in cotton. Phytopathology 59:1119–1127
    [Google Scholar]
  2. Cooper R.M. 1983; The mechanisms and significance of enzymic degradation of host cell walls by parasites. In Biochemical Plant Pathology pp. 101–135 Edited by Callow J.A. Chichester, UK: John Wiley & Sons.;
    [Google Scholar]
  3. Cooper R.M., Rankin B., Wood R.K.S. 1978; Cell wall-degrading enzymes of vascular wilt fungi. 11. Properties and modes of action of polysaccharides of Verticillium albo-atrum and Fusarium oxysporumf.sp. lycopersici. Physiological Plant Pathology 13:101–134
    [Google Scholar]
  4. Cruickshank R.H., Wade G.C. 1980; Detection of pectic enzymes in pectin-acrylamide gels. Analytical Biochemistry 107:177–181
    [Google Scholar]
  5. Evans E.J., Framingham E.E. 1979; Method of determining strain pathogenicity of Verticillium alboatrum wilt of hops. Technical Bulletin, Ministry of Agriculture, Fisheries and Food, South Eastern Region
    [Google Scholar]
  6. Harris R.V. 1961; Progress in research on Verticillium wilt and virus diseases of hops in 1960. Report East Mailing Research Station for 1960 pp. 141–144
    [Google Scholar]
  7. Hastie A.C. 1968; Phialide analysis of mitotic recombination in Verticillium. Molecular and General Genetics 102:232–240
    [Google Scholar]
  8. Isaac I., Keyworth W.G. 1948; Verticillium wilt of hop (Humuluslupulus). III. A study of the pathogenicity of isolates from fluctuating and from progressive outbreaks. Annals of Applied Biology 35:243–249
    [Google Scholar]
  9. Keyworth W.G. 1942; Verticillium wilt of the hop (Humuluslupulus). Annals of Applied Biology 29:346–357
    [Google Scholar]
  10. Lisker N., Retig N. 1974; Detection of polygalacturonase and pectin lyaseisoenzymes in polyacrylamide gels. Journal of Chromatography 96:245–249
    [Google Scholar]
  11. Miller G.L. 1959; Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31:426–428
    [Google Scholar]
  12. Mohan S, Ride J.P. 1982; An immunoelectro-phoretic approach to the identification of progressive and fluctuating isolates of the hop wilt fungus Verticillium albo-atrum. Journal of General Microbiology 128:255–265
    [Google Scholar]
  13. Mohan S.B., Ride J.P. 1983; Fluorescent-antibody staining of conidia of isolates of Verticillium albo-atrumin relation to their virulence for hop. Journal of General Microbiology 129:3581–3589
    [Google Scholar]
  14. Pegg G.F. 1981; Biochemistry and physiology of pathogenesis. In Fungal Wilt Diseases of Plants pp. 193–253 Edited by Mace M.E., Bell A.A., Beckman C.H. New York: Academic Press;
    [Google Scholar]
  15. Schnathorst W.C. 1963; Theoretical relationships between inoculum potential and disease severity based on a study of the variation in virulence among isolates of Verticillium albo-atrum. Phytopathology 53:888
    [Google Scholar]
  16. Sewell G.F.W., Wilson J.F. 1964; The occurrence and dispersal of Verticillium conidia in xylem sap of the hop (Humuluslupulus). Nature; London: 204901
    [Google Scholar]
  17. Talboys P.W. 1960; A culture medium aiding the identification of Verticillium albo-atrumand V.dahliae. Plant Pathology 9:57–58
    [Google Scholar]
  18. Talboys P.W., Busch L.V. 1970; Pectic enzymes produced by Verticilliumspecies. Transactions of the British Mycological Society 55:367–381
    [Google Scholar]
  19. Talboys P.W., Wilson J.F. 1954; A method for determining the pathogenicity of strains of Verticilliumalbo-atrumisolates from hop. Report East Mailing Research Station for 1953158–161
    [Google Scholar]
  20. Typas M.A., Heale J.B. 1976; Heterokaryosis and the role of cytoplasmic inheritance in dark resting structure formation in Verticillium species. Molecular and General Genetics 146:17–26
    [Google Scholar]
  21. Typas M.A., Heale J.B. 1979; Transfer of a cytoplasmic factor by micro-injection in Verticillium. Journal of General Microbiology 111:375–386
    [Google Scholar]
  22. Vogel H.J. 1956; A convenient growth medium for Neurospora. Microbial Genetics Bulletin 13:42–43
    [Google Scholar]
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