1887

Abstract

High-resolution two-dimensional polyacrylamide gel electrophoresis was used to analyse the soluble proteins from seven strains of , six strains of and one or two strains of twelve other species. Approximately 200 individual polypeptides could be visualized as Coomassie Blue stained spots on an electrophoretogram of and similar numbers were found for the other bacteria. Each species of bacterium had a distinctly different pattern of spots which could be recognized. Quantitative comparisons of 48 selected spots derived from one strain of with those of five other strains of gonococcus, three strains of and one of , showed relationships in agreement with their current taxonomic classification but with a higher level of discrimination than that of previously used methods. It was also possible to distinguish the individual gonococcal strains. It is suggested that the method could be useful for bacterial classification and identification.

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1984-12-01
2024-12-14
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References

  1. Ames G.F.-L., Nikaido K. 1976; Two-dimensional gel electrophoresis of membrane proteins. Biochemistry 15:616–623
    [Google Scholar]
  2. Anderson N.G., Anderson N.L. 1978a; Analytical techniques for cell fractions XXI.Twodimensional analysis of serum and tissue proteins.Multiple isoelectric focusing. Analytical Biochemistry 85:331–341
    [Google Scholar]
  3. Anderson N.G., Anderson N.L. 1978b; Analytical techniques for cell fractions XXII.Twodimensional analysis of serum and tissue proteins.Multiple gradient-slab electrophoresis. Analytical Biochemistry 85:341–354
    [Google Scholar]
  4. Benson D.R., Bucholz S.E., Hanna D.G. 1984; Identification of Frankia strains by twodimensional polyacrylamide gel electrophoresis. Applied and Environmental Microbiology 47:489–494
    [Google Scholar]
  5. Catlin B.W. 1970; Transfer of the organism named Neisseria catarrhalis to Branhamella gen.nov. International Journal of Systematic Bacteriology 20:155–159
    [Google Scholar]
  6. Catlin B.W., Cunningham L.S. 1961; Transforming activities and base contents of deoxyribonucleate preparations from various Neisseriae. Journal of General Microbiology 26:303–312
    [Google Scholar]
  7. Demarco De Hormaeche R., Thornley M.J., Glauert A.M. 1978; Demonstration by light and electron microscopy of capsules on gonococci recently grown in vivo. Journal of General Microbiology 106:81–91
    [Google Scholar]
  8. Dunn M.J., Burghes A.H.M. 1983a; High resolution two-dimensional polyacrylamide gel electrophoresis I.Methodological procedures. Electrophoresis 4:97–116
    [Google Scholar]
  9. Dunn M.J., Burghes A.H.M. 1983b; High resolution two-dimensional polyacrylamide gel electrophoresis II.Analysis and applications. Electrophoresis 4:173–189
    [Google Scholar]
  10. Greaves R.I.N. 1960; Preservation of living cells by freeze-drying. Annals of the New York Academy of Sciences 85:723–728
    [Google Scholar]
  11. Hoke C.., Vedros N.A. 1982; Taxonomy of the Neisseriae: deoxyribonucleic acid base composition, interspecific transformation, and deoxyribonucleic acid hybridisation. International Journal of Systematic Bacteriology 32:57–66
    [Google Scholar]
  12. Jackman P.J.H. 1982; Classification of Corynebac-terium species from axillary skin by numerical analysis of electrophoretic protein patterns. Journal of Medical Microbiology 15:485–492
    [Google Scholar]
  13. Jackson P., Thompson R.J. 1981; The demonstration of new human brain specific proteins by high-resolution two-dimensional polyacrylamide gel electrophoresis. Journal of Neurological Science 49:429–438
    [Google Scholar]
  14. Johnston K.H., Holmes K.K., Gotschlich E.C. 1976; The serological classification of Neisseria gonorrhoeae. I.Isolation of the outer membrane complex responsible for serotypic specificity. Journal of Experimental Medicine 143:741–758
    [Google Scholar]
  15. Jones D., Sackin M.J. 1980; Numerical methods in the classification and identification of bacteria with special reference to the Enterobacteriaceae. In Microbiological Classification and Identification pp. 73–106
    [Google Scholar]
  16. Kersters K., De Ley J. 1980; Classification and identification of bacteria by electrophoresis of their proteins. In Microbiological Classification and Identification pp. 273–297 Goodfellow M., Board R.G. Edited by London: Academic Press;
    [Google Scholar]
  17. Kingsbury D.T. 1967; Deoxyribonucleic acid homologies among species of the genus Neisseria. Journal of Bacteriology 94:870–874
    [Google Scholar]
  18. Kingsbury D.T., Fanning G.R., Johnson K.E., Brenner D.J. 1969; Thermal stability of interspecies Neisseria DNA duplexes. Journal of General Microbiology 55:201–208
    [Google Scholar]
  19. Mouches C., Vignault J.C., Tully R.F., Whitcomb R.F., Bove J.M. 1979; Characterization of Spiroplasmas by one and two-dimensional protein analysis on polyacrylamide slab gels. Current Microbiology 2:74–96
    [Google Scholar]
  20. O’Farrell P.H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  21. O’Reilly R.J., Welch B.G., Kellogg D.S.Jr 1973; An indirect fluorescent-antibody technique for the study of uncomplicated gonorrhoea.II.Selection and characterization of the strain of Neisseria gonorrhoeae used as antigen. Journal of Infectious Diseases 127:77–83
    [Google Scholar]
  22. Owen R.J., Jackman P.J.H. 1982; The similarities between Pseudomonas paucimobilis and allied bacteria derived from analysis of deoxyribonucleic acids and electrophoretic protein patterns. Journal of General Microbiology 128:2945–2954
    [Google Scholar]
  23. Reyn A. 1974; Family Neisseriaceae. In Bergey’s Manual of Determinative Bacteriology, 8th edn.. pp. 427–439 Buchanan R E., Gibbons N.E. Edited by Baltimore: Williams, Wilkins;
    [Google Scholar]
  24. Roberts G.P., Leps W.T., Silver L.E., Brill W.J. 1980; Use of two-dimensional polyacrylamide gel electrophoresis to identify and classify Rhizobium strains. Applied and Environmental Microbiology 39:414–422
    [Google Scholar]
  25. Rodwell A.W., Rodwell E.S. 1978; Relationships between strains of Mycoplasma mycoidessubspp.mycoides and capri studied by two-dimensional gel electrophoresis of cell proteins. Journal of General Microbiology 109:259–263
    [Google Scholar]
  26. Sanderson K.E. 1976; Genetic relatedness in the family Enterobacteriaceae. Annual Review of Microbiology 30:327–349
    [Google Scholar]
  27. Skerman V.B.D, Sneath P.H.A. 1980; Approved lists of bacterial names. International Journal of Systematic Bacteriology 30:225–420
    [Google Scholar]
  28. Sneath P.H.A., Sokal R.R. 1962; Numerical taxonomy. Nature; London: 193855–860
    [Google Scholar]
  29. Taylor J., Anderson N.L., Scandora A.E.Jr Willard K.E., Anderson N.G. 1982; Design and implementation of a prototype human protein index. Clinical Chemistry 28:861–866
    [Google Scholar]
  30. Thornley M.J., Wilson D.V., Demarco De Hormaeche R., Oates J.K., Coombs R.R.A. 1979; Detection of gonococcal antigens in urine by radioimmunoassay. Journal of Medical Microbiology 12:161–175
    [Google Scholar]
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