Summary: A plasmid, pCL34, capable of autonomous replication in and has been constructed which carries the promoter and structural gene () for amidase, but not the regulator gene (). Plasmid pCL34 has been mobilized from to using the broad host range plasmid RP4. Complementation studies were performed in strains carrying various amidase mutations. Measurements of amidase activity in the recipients under inducing, non-inducing and repressing conditions showed -complementation by the chromosomally located regulator gene product. These results confirmed the positive control model for amidase gene expression. Levels of amidase expression seen during these studies were approximately threefold higher than in the parental, amidase-positive strains.


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