1887

Abstract

Derivatives of strain W3110 with increased tryptophan synthase (TS) activity were constructed. The biosynthesis of serine was shown to limit tryptophan production in minimal medium with indole as precursor. In the presence of serine and indole we obtained a correlation between the specific activity of TS and the specific productivity ( ) of tryptophan. Supplementation of the growth medium with glycine enhanced two-fold. In a strain with high serine hydroxymethyltransferase (SHMT) activity no such increase of tryptophan productivity was observed, although crude extracts from these cells were shown to produce tryptophan with indole, one-carbon units and glycine as precursors. Growth of the strain with high SHMT activity was inhibited in a medium with high glycine concentration. This inhibition could not be released by addition of isoleucine and valine. In a buffer system with permeabilized cells high in specific TS and SHMT activities we did not obtain any tryptophan production in presence of indole, glycine, one-carbon units and cofactors. On the other hand, in a buffer system with indole and serine as precursors we obtained high of tryptophan [13·3 g tryptophan (g dry wt cells) h], which was correlated to the TS specific activity.

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1984-12-01
2024-03-29
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References

  1. Aiba S., Tsunekawa H., Imanaka T. 1982; New approach to tryptophan production by Escherichia coli: Genetic manipulation of composite plasmids in vitro. Applied and Environmental Microbiology 43:289–297
    [Google Scholar]
  2. Bang W.-G., Lang S., Sahm H., Wagner F. 1983a; Production of L-tryptophan by Escherichia coli cells. Biotechnology and Bioengineering 25:999–1011
    [Google Scholar]
  3. Bang W.-G., Behrendt U., Lang S., Wagner F. 1983b; Continuous production of L-tryptophan from indole and L-serine by immobilized Escherichia coli cells. Biotechnology and Bioengineering 25:1013–1025
    [Google Scholar]
  4. Bertani G. 1951; Studies on lysogenesis.I. The mode of phage liberation by lysogenic Escherichia coli. . Journal of Bacteriology 62:293–300
    [Google Scholar]
  5. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  6. Cheesman P., Crosbie G.W. 1966; C, unit biogenesis in Escherichia coli. Biochemical Journal 99:24
    [Google Scholar]
  7. Cohen S.N., Chang A.C.Y., Hsu L. 1972; Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  8. Cosloy S.D., Mcfall E. 1970; L-Serine-sensitive mutants of Escherichia coli K-12. Journal of Bacteriology 103:840–841
    [Google Scholar]
  9. Crosbie G.W. 1966; Biogenesis of Q units in Escherichia coli. Biochemical Journal 99:21–22 p
    [Google Scholar]
  10. Decottignies-LE Marechal P., Calderon-Seguin R., Vandecasteele J.P., Azerad R. 1979; Synthesis of L-tryptophan by immobilized Escherichia coli cells. European Journal of Applied Microbiology and Biotechnology 7:34–44
    [Google Scholar]
  11. Deu J.K., Harvey R.J. 1982; Sources of one-carbon units in the folate pathway of Escherichia coli. Journal of Biological Chemistry 25:1980–1986
    [Google Scholar]
  12. Greene R., Radovich C. 1975; Role of methionine in regulation of serine hydroxymethyltransferase in Escherichia coli. Journal of Bacteriology 124:269–278
    [Google Scholar]
  13. Harvey R.J. 1973; Growth and inhibition of protein synthesis in Escherichia coli in presence of trimethoprim. Journal of Bacteriology 114:309–322
    [Google Scholar]
  14. Hersfield V., Boyer H.W., Yanofsky C., Lovett M.A., Helinski D.R. 1974; Plasmid Col El as a molecular vehicle for cloning and amplification of DNA. Proceedings of the National Academy of Sciences of the United States of America 71:3455–3459
    [Google Scholar]
  15. Izumi Y., Takizawa M., Tani Y., Yamada H. 1982; L-Serine production by resting cells of a methanol-utilizing bacterium. Journal of Fermentation Technology 60:269–276
    [Google Scholar]
  16. Kupersztoch Y.M., Helinski D.R. 1973; A catenate molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli. Biochemical and Biophysical Research Communications 54:1451–1459
    [Google Scholar]
  17. Meedel T.H., Pizer L.I. 1974; Regulation of one-carbon biosynthesis and utilization in Escherichia coli 60653 x. Journal of Bacteriology 118:905–910
    [Google Scholar]
  18. Miller J.H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor New York; Cold Spring Harbor Laboratory:
    [Google Scholar]
  19. Miller B.A., Newman E.B. 1974; Control of serine transhydroxymethylase synthesis in Escherichia coli K-12. Canadian Journal of Microbiology 20:41–47
    [Google Scholar]
  20. Newman E.B. 1970; Metabolism of serine and glycine in Escherichia coli K-12. 1 16:933–940
    [Google Scholar]
  21. Newman E.B., Walker C. 1982; L-Serine degradation in Escherichia coli K-12. A combination of L-serine, glycine and leucine used as a source of carbon 151:777–782
    [Google Scholar]
  22. Newman E.B., Miller B., Kapoor V. 1974; Biosynthesis of single-carbon units in Escherichia coli K-12. Biochimica et biophysica acta 338:529–539
    [Google Scholar]
  23. Newman E.B., Batist G., Fraser J., Isenberg S., Wegman P., .Kappor V. 1976; The use of glycine as nitrogen source by Escherichia coli K-12. Biochimica et biophysica acta 421:97–105
    [Google Scholar]
  24. Newman E.B., Morris J.F., Walker C., Kapoor V. 1981; A mutation affecting L-serine metabolism in E.coli K-12. Molecular and General Genetics 182:143–147
    [Google Scholar]
  25. Omori K., Kakimoto T., Chibata I. 1983; L-Serine production by a mutant of Sarcina albida defective in L-serine degradation. Applied and Environmental Microbiology 45:1722–1726
    [Google Scholar]
  26. Pizer L.I. 1965; Glycine synthesis and metabolism in Escherichia coli. Journal of Bacteriology 89:1145–1150
    [Google Scholar]
  27. Plamann M.D., Rapp W.D., Stauffer G.V. 1983; Escherichia coli K-12 mutants defective in the glycine cleavage enzyme system. Molecular and General Genetics 192:15–21
    [Google Scholar]
  28. Ream L.W., Margossian L., Clark A.J., Hanson F.G., Von meyerburg K. 1980; Genetic and physical mapping of recF in Escherichia coli K-12. Molecular and General Genetics 180:115–121
    [Google Scholar]
  29. Scrimgeour K.G., Huennekens F.M. 1962; Serine hydroxymethylase. Methods in Enzymology 5:838–843
    [Google Scholar]
  30. Skogman G., Nilsson J., Gustafsson P. 1983; The use of a partition locus to increase stability of tryptophan-operon-bearing plasmids in Escherichia coli. Gene 23:105–115
    [Google Scholar]
  31. Smith D.H., Yanofsky C. 1962; Enzymes involved in the biosynthesis of tryptophan. Methods in Enymology 5:794–806
    [Google Scholar]
  32. Spies J.R., Chambers D.C. 1948; Chemical determination of tryptophan. Study of color-forming reaction of tryptophan, p-dimethylaminobenzalde-hyde and sodium nitrite in sulfuric acid solution 20:30–39
    [Google Scholar]
  33. Stauffer G.V. 1983; Regulation of serine, glycine and one-carbon biosynthesis. In Amino Acids Biosynthesis and Genetic Regulation pp. 103–113 Herrmann K. M., Somerville R. L. Edited by London: Addison-Wesley;
    [Google Scholar]
  34. Stauffer G.V., Plamann M.D., Stauffer L.T. 1981; Construction and expression of hybrid plasmids containing the Escherichia coli glyA gene. Gene 4:63–72
    [Google Scholar]
  35. Tribe D.E., Pittard J. 1979; Hyperproduction of tryptophan by Escherichia coli: Genetic manipulation of the pathways leading to tryptophan formation. Applied and Environmental Microbiology 38:181–190
    [Google Scholar]
  36. Tsunekawa H., Tateishi M., Imanaka T., Aiba S. 1981; TnA -directed deletion of the trp operon from RSF2124-trp in Escherichia coli. Journal of General Microbiology 127:93–102
    [Google Scholar]
  37. Uzan M., Danchin A. 1978; Correlation between the serine sensitivity and derepressability of the Hr genes in Escherichia coli relA mutants. Molecular and General Genetics 165:21–30
    [Google Scholar]
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