1887

Abstract

The Szybalski wedge plate technique was modified to generate two-dimensional diffusion gradients for two environmental variables: pH value and NaCl concentration were selected in this study. After experimentation, pH values chosen ranged from 3·9 to 8·1 and salt concentrations from 23 to 79 gl. The pH gradients were slightly sigmoidal whilst the salt gradients were approximately linear. The reproducibility of the technique was assessed and found to be satisfactory in replicate growth experiments. A wide range of growth patterns was observed for a large variety of different bacterial types. The growth boundary and the pattern details are sufficiently distinct to suggest that the technique can distinguish between closely related species or strains.

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/content/journal/micro/10.1099/00221287-130-11-2921
1984-11-01
2024-04-25
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References

  1. Caldwell D.E, Hirsch P. 1973; Growth of microorganisms in two-dimensional steady-state diffusion gradients. Canadian Journal of Microbiology 19:53–58
    [Google Scholar]
  2. Caldwell D.E., Lai S.H, Tibdje J.M. 1973; A two-dimensional steady-state diffusion gradient for ecological studies. Bulletin of Ecology Research Communication (Stockholm) 17:151–158
    [Google Scholar]
  3. Hutchinson G.E. 1957 A Treatise on Limnology 1 New York: John Wiley;
    [Google Scholar]
  4. Hutchinson G.E. 1965 The Ecological Theater and the Evolutionary Play pp. 26–78 Newhaven: Yale University Press;
    [Google Scholar]
  5. Skerman V.B.D., Mcgowan V., Sneath P.H.A. 1980; Approved lists of bacterial names. International Journal of Systematic Bacteriology 30:225–420
    [Google Scholar]
  6. Szybalski W. 1952; Gradient plates for the study of microbial resistance to antibiotics. Bacteriological Proceedings 36:
    [Google Scholar]
  7. Szybalski W., Bryson V. 1952; Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics. Journal of Bacteriology 64:489–499
    [Google Scholar]
  8. Wimpenny J.W.T. 1981; Spatial order in microbial ecosystems. Biological Reviews 56:295–342
    [Google Scholar]
  9. Wimpenny J.W.T., Lovitt R.W., Coomes J.P. 1983; Laboratory model systems for the investigation of spatially and temporally organised microbialecorystemr. Symposia of the Society for General microbilogy 34:67–117
    [Google Scholar]
  10. Wimpenny J.W.T., Coomes J.P., Lovitt R.W. 1984; Growth and interactions of microorganisms in spatially heterogeneous ecosystems. In Current Perspectives in Microbial Ecology pp. 291–299 Klug M.J., Reddy C.A. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
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