1887

Abstract

A sedimentation chamber and Andersen sampler were used to isolate a range of actinomycetes on selective and non-selective media. The occurrence of different actinomycete groups in natural substrates was compared and strains were screened for the ability to degrade ball-milled straw or to grow on lignin-related phenolic compounds. Evidence for ligninolytic activity in representatives of several genera was obtained by assaying CO evolution from [C]lignin-labelled wheat lignocellulose. Most of the straw-degrading isolates were assigned to the genera and , but only representatives of the latter were found to be active against [C]lignin. Of the non-straw-degrading strains also examined, some which could utilize phenolic substrates produced CO from the [-C]lignocellulose, and two of these were selected for further study. These strains, and a sp., attacked [C]lignin yielding CO and water-soluble C-labelled compounds during primary growth. This activity was not accounted for by the utilization of phenolic acids linked to the carbohydrate fraction of wheat lignocellulose and was unaffected by cultural parameters known to influence lignin degradation by white-rot fungi.

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1984-11-01
2021-10-23
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References

  1. Ander P., Eriksson K.-E., Yu H.-S. 1983; Physiological requirements for degradation of lignin and lignin-related substances by Sporolrichum pulverulentum. European Journal of Applied Microbiology and Biotechnology 18:374–380
    [Google Scholar]
  2. Antai S.P, Crawpord D. L. 1981; Degradation of softwood, hardwood and grass lignocelluloses by two Streptomyces strains. Applied and Environmental Microbiology 42:378–380
    [Google Scholar]
  3. Athalye M., Goodfellow M., Blakey L., Lacey J. 1980; Selective isolation of Actinomadura and related actinomycetes from soil. Journal of Applied Bacteriology 48:xv
    [Google Scholar]
  4. Barder M.J., Crawford D.L. 1981; Effects of carbon and nitrogen supplementation on lignin and cellulose decomposition by a Streptomyces. Canadian Journal of Microbiology 27:859–863
    [Google Scholar]
  5. Crawford D.L. 1978; Lignocellulose decomposition by selected Streptomyces strains. Applied and Environmental Microbiology 35:1041–1045
    [Google Scholar]
  6. Crawford D.L. 1981; Microbial conversions of lignin to useful chemicals using a lignin-degrading Streptomyces. Biotechnology and Bioengineering Symposium 11:275–291
    [Google Scholar]
  7. Crawford D.L., Crawford R.L. 1976; Microbial degradation of lignocellulose: the lignin component. Applied and Environmental Microbiology 31:714–717
    [Google Scholar]
  8. Crawford D.L, Pometto A.L. III Crawford R.L. 1983; Lignin degradation by Streptomyces viridosporus: isolation and characterisation of a new polymeric lignin degradation intermediate. Applied and Environmental Microbiology 45:898–904
    [Google Scholar]
  9. Cross T. 1968; Thermophilic actinomycetes. Journal of Applied Bacteriology 31:36–53
    [Google Scholar]
  10. Deschamps A.M., Gillie J.M., Lebeault J.M. 1981 Direct delignification of untreated bark chips with mixed cultures of bacteria 13:222–225
    [Google Scholar]
  11. Forney L.J., Reddy C.A., Tien M., Aust S.D. 1982; The involvement of hydroxyl radical derived from hydrogen peroxide in lignin degradation by the white-rot fungus Phanerochaete chrysosporium. Journal of Biological Chemistry 257:11455–11462
    [Google Scholar]
  12. Haider K., Trojanowski J., Sundman V. 1978; Screening for lignin degrading bacteria by means of l4C-labelled lignins. Archives of Microbiology 119:103–106
    [Google Scholar]
  13. Higuchi T., Ito T., Shimada M., Kawamura I. 1967; Chemical properties or milled wood lignin of grasses. Phytochemistry 6:1551–1556
    [Google Scholar]
  14. Kirk T.K., Fenn P. 1982; Formation and action of the ligninolytic system in basidiomycetes. In Decomposer Basidiomycetes pp. 69–90 Edited by Franktand J. C., Hedger J. N., Swift M. J. Cambridge: Cambridge University Press;
    [Google Scholar]
  15. Lacey J. 1973; Actinomycetes in soils, composts and fodders. In Actinomycetes: Characteristics and Practical Importance pp. 231–251 Edited by Sykes G., Skinner F. A. London: Academic Press;
    [Google Scholar]
  16. Mccarthy A.J., Cross T. 1981; A note on a selective isolation medium for the thermophilicactinomycete Thermomonosporachromogena. Journal of Applied Bacteriology 51:299–302
    [Google Scholar]
  17. Mccarthy A.J., Cross T. 1984; A taxonomic study of Thermomonospora and other monosporic actinomycetes. Journal of General Microbiology 130:5–25
    [Google Scholar]
  18. Mccarthy A.J., Macdonald M.J., Paterson A., Broda P. 1984; Degradation of [l4C]lignin-labelled wheat lignocetlulose by white-rot fungi. Journal of General Microbiology 130:1023–1030
    [Google Scholar]
  19. Orchard V.A. 1980; Long term effect of sewage sludge additions on populations of Nocardia asteroides, Micromonospora and Thermoactinomyces in soil. Soil Biology and Biochemistry 12:477–482
    [Google Scholar]
  20. Phelan M.B., Crawford D.L., Pometto A.L. III 1979; Isolation of lignocellulose-decomposing actinomycetes and degradation of specifically l4C- labelled lignocellulose by six selected Slreptomyces strains. Canadian Journal of Microbiology 25:1270–1276
    [Google Scholar]
  21. Reid I.D., Abrams G.D., Pepper J.M. 1982; Water-soluble products from the degradation of aspen lignin by Phanerochaete chrysosporium. Canadian Journal of Botany 60:2357–2364
    [Google Scholar]
  22. Rowbotham T.J., Cross T. 1977; Ecology of Rhodococcus coprophilus and associated actinomycetes in fresh water and agricultural habitats. Journal of General Microbiology 100:231–240
    [Google Scholar]
  23. Sandrak N.A. 1977; Cellulose decomposition by Micromonosporas.(In Russian with English summary). Mikrobiologiya 46:478–481
    [Google Scholar]
  24. Stutzenberger F.J. 1972; Cellulolytic activity of Thermomonosporacurvata: nutritional requirements for cellulase production. Applied Microbiology 24:77–82
    [Google Scholar]
  25. Sundman V., Näse L. 1971; A simple plate test for direct visualisation of biological lignin degradation. Papperi Ja Puu 2:67–71
    [Google Scholar]
  26. Tien M., Kirk T.K. 1983; Lignin degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science 221:661–663
    [Google Scholar]
  27. Trojanowski J., Haider K., Sundman V. 1977; Decomposition of l4C-labelled lignin and phenols by a Nocardia sp. Archives of Microbiology 114:149–153
    [Google Scholar]
  28. Waksman S.A., Cordon T.C. 1939; Thermophilic decomposition of plant residues in composts by pure and mixed cultures of microorganisms. Soil Science 47:217–224
    [Google Scholar]
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