1887

Abstract

Resistance to the endogenous antibiotic was studied in three actinomycetes that produce inhibitors of RNA polymerase. The three producers, (rifamycin producer), (streptovaricin producer) and (streptolydigin producer), were each highly resistant to the antibiotic they produce (MIC >200 μg ml) and RNA synthesis was also resistant. However, cross-resistance to the other RNA polymerase inhibitors was not found. Resistance to these antibiotics was due to target site modification, since the RNA polymerase enzymes of the three producing organisms were highly resistant to the corresponding antibiotic, and no antibiotic-inactivating enzymes were detected. A mutant was isolated from which was sensitive to steptovaricin (its own product) and also showed an increased sensitivity to rifamycin and streptolydigin. This mutant had RNA polymerase which was extremely sensitive to the three antibiotics.

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1984-11-01
2021-08-05
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References

  1. Babinbt C. 1967; A new method for the purification of RNA polymerase. Biochemical and Biophysical Research Communications 26:639–644
    [Google Scholar]
  2. Benveniste R., Davies J. 1973; Aminoglycoside-antibiotic inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria. Proceedings of the National Academy of Sciences of the United States of America 70:2276–2280
    [Google Scholar]
  3. Boothroyd C.M., Malet R.M., Nene V., Glass R.E. 1983; Genetic studies on the subunit of β Escherichia coli RNA polymerase. III. Analysis of low level rifampicin-resistant mutants. Molecular and General Genetics 190:523–526
    [Google Scholar]
  4. Boyd D.H., Zillig W., Scajfe F.J.G. 1974; Reference mutations for the β subunit of RNA polymerase. Molecular and General Genetics 130:315–320
    [Google Scholar]
  5. Cassani G., Burgess R.R., Goodman H.M., Gold L. 1971; Inhibition of RNA polymerase by streptoiydigin. Nature New Biology 230:197–200
    [Google Scholar]
  6. Chater K.F. 1974; Rifampicin-resistant mutants of Streptomyces coelicolor A3(2). Journal of General Microbiology 80:277–290
    [Google Scholar]
  7. Cundliffe E. 1978; Mechanism of resistance to thiostrepton in the producing-organism Streptomyces azureus. Nature London; 272:792–795
    [Google Scholar]
  8. Cundliffe E., Thompson J. 1981; The mode of action of nosiheptide (multhiomycin) and the mechanism of resistance in the producing organism. Journal of General Microbiology 126:185–192
    [Google Scholar]
  9. Dabbs E.R. 1979; New tool for studying interactions of components of ribonucleic acid polymerase: rifampin-dependent mutants. Journal of Bacteriology 139:1072–1074
    [Google Scholar]
  10. Halling S.M., Burtis K.C., Doi R.H. 1977; Reconstitution studies show that rifampicin resistance is determined by the largest polypeptide of Bacillus subtilis RNA polymerase. Journal of Biological Chemistry 252:9024–9031
    [Google Scholar]
  11. Halling S.M., Burtis K.C., Doi R.H. 1978; β subunit of bacterial RNA polymerase is responsible for streptoiydigin resistance in Bacillus subtilis. Nature, London 272:837–839
    [Google Scholar]
  12. Hardisson C., Salas J.A., Guijarro J.A., Suárez J.E. 1980; Macromolecular synthesis during the germination of Streptomyces spores in a chemically defined medium. FEMS Microbiology Letters 7:233–235
    [Google Scholar]
  13. Helm K., VonDer J.S, Krakow J.S. 1972; Inhibition of RNA polymerase by streptoiydigin. Nature New Biology 235:82–83
    [Google Scholar]
  14. Herbert C.J., Giles I.G., Akhtar M. 1983; The sequence of an antibiotic resistance gene from an antibiotic-producing bacterium: homologies with transposon genes. FEBS Letters 160:67–71
    [Google Scholar]
  15. Hotta K., Yamamoto H., Okami Y.A., Umezawa H. 1981; Resistance mechanisms of kanamycin-, neomycin-, and streptomycin-producing streplo-mycetes to aminoglycoside antibiotics. Journal of Antibiotics 34:1175–1182
    [Google Scholar]
  16. Iwakura Y., Ishihama A.A., Yura T. 1973; RNA polymerase mutants of Escherichia coli. II. Streptoiydigin resistance and its relation to rifampicin resistance. Molecular and General Genetics 121:181–196
    [Google Scholar]
  17. Kessler C., Hartmann G.R. 1977; The two effects of rifampicin on the RNA polymerase reaction. Biochemical and Biophysical Research Communications 74:50–56
    [Google Scholar]
  18. Linn T., Losick RA., Sonenshein A.L. 1975; Rifampicin resistance mutation of Bacillus subtilisaltering the electrophoretic mobility of the β subunit of ribonucleic acid polymerase. Journal of Bacteriology 122:1387–1390
    [Google Scholar]
  19. Malik V.S.A., Vining L.C. 1972; Chloramphenicol resistance in a chloramphenicol-producing Streptomyces. Canadian Journal of Microbiology 18:583–590
    [Google Scholar]
  20. Meyenburg K., Von Nielsen L.D., Johnsen K., Moli S., Svenningsen B., Miozzari G. 1978; Reevaluation of the mode of action of streptoiydigin in Escherichia coli: induction of transcription termination in vivo. Antimicrobial Agents and Chemotherapy 13:234–243
    [Google Scholar]
  21. Ovchinnikov Y.A., Monastyrskaya G.S., Gurier S.O., Kalinina N.F., Sverdlow E.D., Gra-Gerov A.I., Bass I.A., Kiver I.F., Moiseyeva E.P., Igumnov V.N., Mindlin S.Z., Nikiforov V.G., Khesin R.B. 1983; RNA polymerase rifampicin resistance mutations in Escherichia coli:sequence changes and dominance. Molecular and General Genetics 190:344–348
    [Google Scholar]
  22. Pérez-González J.A., Vara J., Jiménez A. 1983; Acetylation of puromycin by Streptomyces alboniger the producing organism. Biochemical and Biophysical Research Communications 113:772–777
    [Google Scholar]
  23. Piendl W., Bock A. 1982; Ribosomal resistance in the gentamicin producer organism Micro-monosporapurpurea. Antimicrobial Agents and Chemotherapy 22:231–236
    [Google Scholar]
  24. Rabussay D.A., Zillig W. 1969; A rifampicin resistant RNA polymerase from Escherichia coli altered in the β subunit. FEBS Letters 5:104–106
    [Google Scholar]
  25. Schleif R. 1969; Isolation and characterization of a streptoiydigin resistant RNA polymerase. Nature New Biology 223:1068–1069
    [Google Scholar]
  26. Siddhikol C., Erbstoezer J.W., Weisblum B. 1969; Mode of action of streptoiydigin. Journal of Bacteriology 99:151–155
    [Google Scholar]
  27. Sippel A., Hartmann G.R. 1968; Mode of action of rifamycin on the RNA polymerase reaction. Biochimica et biophysica acta 157:218–219
    [Google Scholar]
  28. Skinner R.H.A., Cundliffe E. 1980; Resistance to the antibiotics viomycin and capreomycin in the Streptomyces species which produce them. Journal of General Microbiology 120:95–104
    [Google Scholar]
  29. Skinner R.H.A., Cundliffe E. 1982; Dimethylation of adenine and the resistance of Streptomyces erythreus to erythromycin. Journal of General Microbiology 128:2411–2416
    [Google Scholar]
  30. Sonenshein A.L., Brevet J.A., Cote R. 1974; Isolation and characterization of rifampicin-resistant and streptolydigin-resistant mutants of Bacillus subtilis with altered sporulation properties. Journal of Bacteriology 120:253–265
    [Google Scholar]
  31. Thompson J.A., Cundliffe E. 1980; Resistance to thiostrepton, siomycin and sporangiomycin in actinomycetes that produce them. Journal of Bacteriology 142:455–461
    [Google Scholar]
  32. Thompson C.J.A., Gray J. 1983; Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids. Proceedings of the National Academy ofSciences of the United States of America 80:5190–5194
    [Google Scholar]
  33. Thompson J., Cundliffe E.A., Stark M. 1982; On the mode of action of beminamycin and the mechanism of resistance in the producing organism.Streptomyces bernensis. Journal of General Microbiology 128:875–884
    [Google Scholar]
  34. Vining L.C. 1979; Antibiotic tolerance in producer organisms. Advances in Applied Microbiology 25:147–168
    [Google Scholar]
  35. Walker M.S.A., Walker J.B. 1970; Streptomycin biosynthesis and metabolism. Journal of Biological Chemistry 245:6683–6689
    [Google Scholar]
  36. Watanabe S., Tanaka K. 1976; The effect of rifampicin on in vitro RN A synthesis of Streptomyces mediterranei. Biochemical and Biophysical Research Communications 72:522–529
    [Google Scholar]
  37. Wehrli W., Knusel F., Schmid K., Staehelin M. 1968; Interaction of rifamycin with bacterial RNA polymerases. Proceedings of the National Academy of Sciences of the United States of America 61:667–673
    [Google Scholar]
  38. Yura T., Igarashi K. 1968; RNA polymerase mutants of Escherichia coli. I. Mutants resistant to streptovaricin. Proceedings of the National Academy of Sciences of the United States of America 61:1313–1319
    [Google Scholar]
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