SUMMARY: Mutants of supersensitive to the nicotinic acid analogue 6-aminonicotinic acid (6ANA) were isolated as unable to grow on what are normally subinhibitory concentrations of the analogue. The mutations were classified on the basis of their map positions as (89-92 units), (66-69 units), (18-22 units), (18 units) and (55 units). The mutants exhibited a wide range of minimal inhibitory concentrations towards 6ANA, and several were affected in terms of growth. The data suggest that most of the mutations probably reside in genes whose products utilize nicotinamide adenine dinucleotide (NAD) as a cofactor, the altered gene products being more sensitive to internal 6-amino NAD concentrations. Secondary mutations which suppress the Pas phenotype were found to reside in the following NAD-related loci; and Two of the mutants appear to be affected in the expression of NAPRTase while several of the mutants are apparently insensitive to feedback inhibition by internal NAD concentrations.


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