1887

Abstract

Mutants of supersensitive to the nicotinic acid analogue 6-aminonicotinic acid (6ANA) were isolated as unable to grow on what are normally subinhibitory concentrations of the analogue. The mutations were classified on the basis of their map positions as (89–92 units), (66–69 units), (18–22 units), (18 units) and (55 units). The mutants exhibited a wide range of minimal inhibitory concentrations towards 6ANA, and several were affected in terms of growth. The data suggest that most of the mutations probably reside in genes whose products utilize nicotinamide adenine dinucleotide (NAD) as a cofactor, the altered gene products being more sensitive to internal 6-amino NAD concentrations. Secondary mutations which suppress the Pas phenotype were found to reside in the following NAD-related loci; and Two of the mutants appear to be affected in the expression of NAPRTase while several of the mutants are apparently insensitive to feedback inhibition by internal NAD concentrations.

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/content/journal/micro/10.1099/00221287-130-11-2873
1984-11-01
2024-03-29
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References

  1. Chan R.K., Botstein D., Watanabe T., Ogata Y. 1972; Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. Virology 50:883–898
    [Google Scholar]
  2. Cobb J.R., Pbarcy S.C., Gholson R.K. 1977; Metabolism of 6-amino nicotinic acid in Escherichia coli. Journal of Bacteriology 131:789–794
    [Google Scholar]
  3. Foster J.W, Moat A.G. 1980; Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems. Microbiological Reviews 44:83–105
    [Google Scholar]
  4. Foster J.W., Kinney O.M, Moat A.G. 1979a; Pyridine nucleotide cycle of Salmonella typhimurium: isolation and characterization of pncA, pncB and pncC mutants and utilization of exogenous nicotinamide adenine dinucleotide. Journal of Bacteriology 137:1165–1175
    [Google Scholar]
  5. Foster J.W., Kinney D.M, Moat A.G. 1979b; Pyridine nucleotide cycle of Salmonella typhimurium : regulation of nicotinic acid phosphoribosyl-transferase and nicotinamide deamidase. Journal of Bacteriology 138:957–961
    [Google Scholar]
  6. Holley E.A, Foster J.W. 1982; Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions. Journal of Bacteriology 152:959–962
    [Google Scholar]
  7. Hughes K.T., Cookson B.T., Ladika D., Olivera B.M, Roth J.R. 1983a; 6-Aminonicotinamide-resistant mutants of Salmonella typhimurium. Journal of Bacteriology 154:1126–1136
    [Google Scholar]
  8. Hughes K.T, Ladika D., Roth J.R, Olivera B.M. 1983b; An indispensable gene for NAD biosynthesis in Salmonella typhimurium. Journal of Bacteriology 155:213–221
    [Google Scholar]
  9. Imsande J. 1964; A comparative study of the regulation of pyridine nucleotide formation. Biochimica et biophyska acta 82:445–453
    [Google Scholar]
  10. Kleckner N., Roth J., Botstein D. 1977; Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. Journal of Molecular Biology 116:125–159
    [Google Scholar]
  11. Miller J.H.ed. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Pullman B., Pullman A. 1959; On the 6-amino nicotinamide antagonisms of DPN-dependent enzymatic systems. Cancer Reaserch 19:337–338
    [Google Scholar]
  13. Sanderson K.E., Hartman P.E. 1978; Linkage map of Salmonella typhimurium,. , V. Microbiologkal Reviews 42:471–519
    [Google Scholar]
  14. Schmieger H. 1971; A method for detection of phage mutants with altered transducing ability. Molecular and General Genetics 110:378–381
    [Google Scholar]
  15. Vogel H.J, Bonner D.M. 1956; Acetyl omithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 93:237–244
    [Google Scholar]
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