1887

Abstract

The obligate intracellular rickettsia, , was shown to possess an energy dependent proline transport system which displayed a high degree of specificity and was highly dependent on pH. Transport was maximal at pH 3·0 to 4·5, a pH range approximating that of the host cell phagolysosome where the agent replicates. Transport was inhibited by the uncouplers carbonyl cyanide -chlorophenylhydrazone and dinitrophenol, but not by sodium arsenite. In the presence of glutamate, a preferred energy source, proline uptake was enhanced more than two-fold. This enhancement of proline uptake was greatly decreased in the presence of sodium arsenite. The addition of glutamate decreased the apparent for proline transport from 45 μm to 15 μm, with the increasing from 3μ6 pmol s (mg dry wt) to 4·8 pmol s (mg dry wt). Two proline analogues, furoic acid and azetidine-2-carboxylic acid, were effective inhibitors of proline transport. -Proline, 4-hydroxyproline, glycine and proline amide inhibited transport minimally, while no inhibition was seen with succinate, pyruvate or glutamate.

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1984-11-01
2021-10-27
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References

  1. Anderson R.R, Menzel R., Wood J.M. 1980; Biochemistry and regulation of a second l-proline transport system in Salmonella typhimurium. Journal of Bacteriology 141:1071–1076
    [Google Scholar]
  2. Baca O.G., Paretsky D. 1983; Q fever and Coxiella burnetii: a model for host-parasite interactions. Microbiological Reviews 47:127–149
    [Google Scholar]
  3. Berger E.A. 1973; Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 70:1514–1518
    [Google Scholar]
  4. Burton P.R., Stueckman J., Welsh R.M., Paretsky D. 1978; Some ultrastructural effects of persistent infection by the rickettsia Coxiella burnetii in mouse L cells and green monkey kidney (VERO) cells. Infection and Immunity 21:557–566
    [Google Scholar]
  5. Dowd J.E., Riggs D.S. 1965; A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations. Journal of Biological Chemistry 240:863–869
    [Google Scholar]
  6. Eisenberg A.D., Marquis R.E. 1981; Enhanced transmembrane proton conductance in Streptococcus mutant GS-5 due to ionophores and fluoride. Antimicrobial Agents and Chemotherapy 19:807–812
    [Google Scholar]
  7. Gimenez D.F. 1964; Staining rickettsiae in yolk-sac cultures. Stain Technology 39:135–140
    [Google Scholar]
  8. Hackstadt T. 1983; Estimation of the cytoplasmic PH of Coxiella burnetii and effect of substrate oxidation on the proton motive force. Journal of Bacteriology 154:591–597
    [Google Scholar]
  9. Hackstadt T., Williams J.C. 1981a; Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii. Proceedings of the National Academy of Sciences of the United States of America 78:3240–3244
    [Google Scholar]
  10. Hackstadt T., Williams J.C. 1981b; Stability of the adenosine 5´-triphosphate pool in Coxiella burnetii: influence of pH and substrate. Journal of Bacteriology 148:419–425
    [Google Scholar]
  11. Hackstadt T., Williams J.C. 1983; pH dependence of the Coxiella burnetii glutamate transport system. Journal of Bacteriology 154:598–603
    [Google Scholar]
  12. Halpern Y.S. 1974; Genetics of amino acid transport in bacteria. Annual Review of Genetics 8:103–133
    [Google Scholar]
  13. Hendrix L.R., Mallavia L.P. 1983; Characterization of a proline active transport system in Coxiella burnetii. In Abstracts of the Annual Meeting of the American Society for Microbiology D37 p. 65 Washington. DC: American Society for Microbiology;
    [Google Scholar]
  14. Kadner R.J., Winkler H.H. 1975; Energy coupling for methionine transport in Escherichia coli. Journal of Bacteriology 123:985–991
    [Google Scholar]
  15. Kay W.W., Gronlund A.F. 1969; Proline transport by Pseudomonas aeruginosa. Biochimica et biophysica acta 193:444–454
    [Google Scholar]
  16. McCaul T.F., Williams J.C. 1981; Developmental cycle of Coxiella burnetii: structure and morphogenesis of vegetative and sporogenic differentiations. Journal of Bacteriology 147:1063–1076
    [Google Scholar]
  17. Ohkuma S., Poole B. 1978; Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proceedings of the National Academy of Sciences of the United States of America 75:3327–3331
    [Google Scholar]
  18. Peterson E.M., Calderone R.A. 1978; Inhibition of specific amino acid uptake in Candida albicans by lysosomal extracts from rabbit alveolar macrophages. Infection and Immunity 21:506–513
    [Google Scholar]
  19. Ribi E., Hoyer B.H. 1960; Purification of Q fever rickettsiae by density-gradient sedimentation. Journal of Immunology 85:314–318
    [Google Scholar]
  20. Rowland I., Tristram H. 1975; Specificity of the Escherichia coli proline transport system. Journal of Bacteriology 123:871–877
    [Google Scholar]
  21. Segel I.H. 1976 Biochemical Calculations, 2nd edn. p. 411 New York: John Wiley & Sons;
    [Google Scholar]
  22. Weiss E. 1982; The biology of rickettsiae. Annual Review of Microbiology 36:345–370
    [Google Scholar]
  23. Williams J.C., Peacock M.G., Mccaul T.F. 1981; Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components. Infection and Immunity 32:840–851
    [Google Scholar]
  24. Winkler H.H., Daugherty R.M. 1984; Proline transport and metabolism in Rickettsia prowazekii. Journal of Bacteriology 158:460–463
    [Google Scholar]
  25. Wood J.M., Zadworny D. 1979; Characterization of an inducible porter required for l-proline catabolism by Escherichia coli K12. Canadian Journal of Biochemistry 57:1191–1199
    [Google Scholar]
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