Location of the Catalytic Site of the Respiratory Fumarate Reductase of Free

Abstract

The location of the catalytic site of the membrane-bound respiratory fumarate reductase of was investigated using mutants and inhibitors of dicarboxylic acid transport. Comparison of apparent and values for fumarate in intact cells and in inverted membrane vesicles showed that externally added fumarate was required to be transported across the cytoplasmic membrane prior to reduction. The catalytic site of fumarate reductase must therefore be located on the cytoplasmic face of the membrane.

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/content/journal/micro/10.1099/00221287-130-11-2851
1984-11-01
2024-03-29
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