1887

Abstract

The location of the catalytic site of the membrane-bound respiratory fumarate reductase of was investigated using mutants and inhibitors of dicarboxylic acid transport. Comparison of apparent and values for fumarate in intact cells and in inverted membrane vesicles showed that externally added fumarate was required to be transported across the cytoplasmic membrane prior to reduction. The catalytic site of fumarate reductase must therefore be located on the cytoplasmic face of the membrane.

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/content/journal/micro/10.1099/00221287-130-11-2851
1984-11-01
2021-10-23
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References

  1. Gutowski S.J., Rosenberg H. 1976; Effects of dicyclohexylcarbodiimide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coti K12. Biochemical Journal 160:813–816
    [Google Scholar]
  2. Gutowski S.J., Rosenberg H. 1977; Proton translocation coupled to electron flow from endogenous substrates to fumarate in anaerobically grown Escherichia coti K12. Biochemical Journal 164:265–267
    [Google Scholar]
  3. Jones R.W., Garland P.B. 1977; Sites and specificity of the reaction of bipyridylium com-pounds with anaerobic respiratory enzymes of Escherichia colt. Effects of permeability barriers imposed by the cytoplasmic membrane. Biochemical Journal 164:199–211
    [Google Scholar]
  4. Kay W.W., Kornberg H.L. 1971; Uptake of C-4- dicarboxylic acids by Escherichia coli. European Journal of Biochemistry 18:274–281
    [Google Scholar]
  5. Lemire B.D., Robinson J.J., Bradley R.D., Scaraba D.G., Weiner J.H. 1983; Structure of fumarate reductase on the cytoplasmic membrane of Escherichia coli. Journal of Bacteriology 155:391–397
    [Google Scholar]
  6. Reid G.A., Ingledew W.J. 1979; Characterization and phenotypic control of the cytochrome content of Escherichia coli. Biochemical Journal 182:465–472
    [Google Scholar]
  7. Spencer M.E., Guest J.R. 1973; Isolation and properties of fumarate reductase mutants of Escherichia coli. Journal of Bacteriology 114:563–570
    [Google Scholar]
  8. Van der plas J., Hellingwerf K.J., Seijen H.G., Guest J.R., Weiner J.H., Konings W.N. 1983; Identification and localisation of enzymes on the fumarate reductase and nitrate respiratory systems of Escherichia coli by crossed immunoelectrophoresis. Journal of Bacteriology 153:1027–1037
    [Google Scholar]
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