Respiratory Adaptation of Anaerobically Grown : Changes in Distribution of Enzymes Free

Abstract

Subcellular fractionations using metrizamide density gradients revealed intermediary stages of respiratory adaptation of grown anaerobically in the presence of the density label 16-bromo-9-hexadecenoic acid. Prior to adaptation, activities of malate dehydrogenase and oligomycin-sensitive ATPase were contained within a membrane population at ρ = 1·20 g ml. After 10 min adaptation cytochrome oxidase activity was associated with these membranes, with ATPase-containing membranes at lower densities (ρ = 1·05 to 1·14 g ml) and with membranes containing malate dehydrogenase at higher density (ρ = 1·24 g ml). After further adaptation these enzymes were associated firstly with two distinct membrane populations at ρ = 1·17 and 1·20 g ml and finally with a single population of mitochondria at ρ = 1·16 g ml. The significance of these changes is discussed in terms of mitochondrial differentiation. Peroxisomes were evident even in early stages of respiratory adaptation and were well separated from mitochondria in later stages.

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1984-11-01
2024-03-29
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References

  1. Ainsworth P.J., Tustanoff R., Ball A.J.S. 1972; Membrane phase transitions as a diagnostic tool for studying mitochondriogenesis. Biochemical and Biophysical Research Communications 47:1299–1305
    [Google Scholar]
  2. Andreasen A.A., Stier T.J.B. 1954; Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 43:271–281
    [Google Scholar]
  3. Blomquist A.T., Holley R.W. 1948; Many membered carbon rings. III. Carboxylic acid derivatives of cycloheptadecane. Journal of the American Chemical Society 701:36–39
    [Google Scholar]
  4. Cartledge T. G., Lloyd D. 1972a; Subcellular fractionation by differential and zonal centrifugation of aerobically grown glucose-derepressedSaccharomyces carlsbergensis. Biochemical Journal 126:381–393
    [Google Scholar]
  5. Cartledge T.G., Lloyd D. 1972b; Subcellular fractionation by zonal centrifugation of glucose-repressed anaerobically grown Saccharomyces carlsbergensis. Biochemical Journal 127:693–703
    [Google Scholar]
  6. Cartledge T.G., Lloyd D. 1973; Changes in enzyme activities and distributions during glucose derepression and respiratory adaptation of anaerobi-cally grown Saccharomyces carlsbergensis. Biochemical Journal 132:609–621
    [Google Scholar]
  7. Duell E.A., Inque S., Utter M.F. 1964; Isolation and properties of intact mitochondria from spheroplasts of yeasts. Journal of Bacteriology 38:1762–1768
    [Google Scholar]
  8. Fiske C.H., Subbarow Y. 1925; The colorimetric determination of phosphorus. Journal of Biological Chemistry 66:375–382
    [Google Scholar]
  9. Fukui S., Tanaka A., Kawamoto S., Yasuhara S., Teranishi Y., Osumi M. 1975; Ultrastructure of methanol-utilizing yeast cells: appearance of microbodies in relation to high catalase activity. Journal of Bacteriology 123:317–328
    [Google Scholar]
  10. Hunsdiecker H. 1943; Beitäge, zur Kenntris makro-cyclischer Ringsysteme, III. Mitteilung: die Synthese des Zibetons. Berichte der Deutschen Chemischen Gesellschaft 76B:142–149
    [Google Scholar]
  11. Jenkins R.O., Cartledge T.G., Lloyd D. 1983; Subcellular fractionation of Candida stellatoideaafter growth with glucose or n-hexadecane. Journal of General Microbiology 129:1171–1185
    [Google Scholar]
  12. Kitto G.B. 1969; Intra and extra-mitochondrial malate dehydrogenase from chicken and tuna heart. Methods in Enzymology 13:106–116
    [Google Scholar]
  13. Plattner H., Schatz G. 1969; Promitochondria anaerobically grown yeasts.III. Morphology Biochemistry 8:339–343
    [Google Scholar]
  14. Plattner H., Salpeter M.M., Saltzgaber J., Schatz G. 1970; Promitochondria of anaerobically grown yeast, IV.Conversion into respiring mitochondria. Proceedings of the National Academy of Sciences of the United States of America 66:1252–1259
    [Google Scholar]
  15. Smith L. 1955; Cytochromes a, a1, a2 and a3. Methods in Enzymology 2:736–740
    [Google Scholar]
  16. Somlo M., Krupa M. 1974; A study of the density pattern of ATPase and respiratory enzymes during mitochondrial biogenesis of Saccharomyces cerevisiae. European Journal of Biochemistry 42:429–437
    [Google Scholar]
  17. Watson K., Haslam J.M., Linnane A.W. 1970; Biogenesis of mitochondria. XII. The isolation of mitochondrial structures from anaerobically grown cells. Journal of Cell Biology 46:88–96
    [Google Scholar]
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