Subcellular fractionations using metrizamide density gradients revealed intermediary stages of respiratory adaptation of Saccharomyces uvarum grown anaerobically in the presence of the density label 16-bromo-9-hexadecenoic acid. Prior to adaptation, activities of malate dehydrogenase and oligomycin-sensitive ATPase were contained within a membrane population at ρ = 1·20 g ml−1. After 10 min adaptation cytochrome c oxidase activity was associated with these membranes, with ATPase-containing membranes at lower densities (ρ = 1·05 to 1·14 g ml−1) and with membranes containing malate dehydrogenase at higher density (ρ = 1·24 g ml−1). After further adaptation these enzymes were associated firstly with two distinct membrane populations at ρ = 1·17 and 1·20 g ml−1 and finally with a single population of mitochondria at ρ = 1·16 g ml−1. The significance of these changes is discussed in terms of mitochondrial differentiation. Peroxisomes were evident even in early stages of respiratory adaptation and were well separated from mitochondria in later stages.
AinsworthP.J., TustanoffR., BallA.J.S.1972; Membrane phase transitions as a diagnostic tool for studying mitochondriogenesis. Biochemical and Biophysical Research Communications 47:1299–1305
AndreasenA.A., StierT.J.B.1954; Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 43:271–281
BlomquistA.T., HolleyR.W.1948; Many membered carbon rings. III. Carboxylic acid derivatives of cycloheptadecane. Journal of the American Chemical Society 701:36–39
CartledgeT.G., LloydD.1973; Changes in enzyme activities and distributions during glucose derepression and respiratory adaptation of anaerobi-cally grown Saccharomyces carlsbergensis. Biochemical Journal 132:609–621
FukuiS., TanakaA., KawamotoS., YasuharaS., TeranishiY., OsumiM.1975; Ultrastructure of methanol-utilizing yeast cells: appearance of microbodies in relation to high catalase activity. Journal of Bacteriology 123:317–328
HunsdieckerH.1943; Beitäge, zur Kenntris makro-cyclischer Ringsysteme, III. Mitteilung: die Synthese des Zibetons. Berichte der Deutschen Chemischen Gesellschaft 76B:142–149
JenkinsR.O., CartledgeT.G., LloydD.1983; Subcellular fractionation of Candida stellatoideaafter growth with glucose or n-hexadecane. Journal of General Microbiology 129:1171–1185
PlattnerH., SalpeterM.M., SaltzgaberJ., SchatzG.1970; Promitochondria of anaerobically grown yeast, IV.Conversion into respiring mitochondria. Proceedings of the National Academy of Sciences of the United States of America 66:1252–1259
SomloM., KrupaM.1974; A study of the density pattern of ATPase and respiratory enzymes during mitochondrial biogenesis of Saccharomyces cerevisiae. European Journal of Biochemistry 42:429–437
WatsonK., HaslamJ.M., LinnaneA.W.1970; Biogenesis of mitochondria. XII. The isolation of mitochondrial structures from anaerobically grown cells. Journal of Cell Biology 46:88–96