1887

Abstract

The kinetics of cell proliferation of a population of a small-size mutant of were compared with those of a wild-type cell population, using time-lapse cinephotomicrography. The mean size of small-size mutant cells was approximately half that of wild-type cells at corresponding points in the cell cycle. The cycle times of small-size mutant cells were much more variable, especially for daughter cells, than those of wild-type cells. The difference in the variability of cycle times of the two strains was mainly due to the different degree of variability of their respective unbudded periods. Like wild-type cells, daughter cells of the small-size mutant were smaller and had a longer cycle time than parent cells. The small-size mutant retains a single size control over the cell cycle.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-11-2803
1984-11-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/11/mic-130-11-2803.html?itemId=/content/journal/micro/10.1099/00221287-130-11-2803&mimeType=html&fmt=ahah

References

  1. Carter B.L.A. 1981; The control of cell division in S. cerevisiae. In The Cell Cycle pp. 99–117 John P.C.L. Edited by John. Cambridge: Cambridge University Press.;
    [Google Scholar]
  2. Carter B.L.A., Jagadish M.N. 1978; The relationship between cell size and ceil division in the yeast Saccharomyces cerevisiae. Experimental Cell Research 112:15–24
    [Google Scholar]
  3. Carter B.L.A., Sudbery P.E. 1980; Small-sized mutants of Saccharomyces cerevisiae. Genetics 96:561–566
    [Google Scholar]
  4. Fantes P.A. 1981; Isolation of cell size mutants of a fission yeast by a new selective method: characterization of mutants and implications for division control mechanisms. Journal of Bacteriology 146:746–754
    [Google Scholar]
  5. Hartwell L.H, Unger M.W. 1977; Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. Journal of Cell Biology 75:422–435
    [Google Scholar]
  6. Hartwell L.H., Culotti J., Pringle J.R., Reid B.J. 1974; Genetic control of the cell division cycle in yeast. Science 183:46–51
    [Google Scholar]
  7. Johnston G.C., Ehrhardt C.W., Lorincz A., Carter B.L.A. 1979; Regulation ofcellsizeinthe yeast Saccharomyces cerevisiae. Journal of Bacteriology 137:1–5
    [Google Scholar]
  8. Lord P.G., Wheals A.E. 1981; Variability in individual cell cycles of Saccharomyces cererisiae. Journal of Cell Science 50:361–376
    [Google Scholar]
  9. Lord P.G., Wheals A.E. 1983; Rate of cell cycle initiation of yeast cells when cell size is not a ratedetermining factor. Journal of Cell Science 59:183–201
    [Google Scholar]
  10. Nurse P. 1975; Genetic control of cell size at cell division in yeast. Nature; London: 256547–551
    [Google Scholar]
  11. Nurse P., Bissett Y. 1981; Gene required in Gl for commitment to cell cycle and in G2 for control of mitosis in fission yeast. Nature; London: 292558–560
    [Google Scholar]
  12. Nurse P., Thuriaux P. 1977; Controls over the timing of DNA replication during the cell cycle of fission yeast. Experimental Cell Research 107:365–375
    [Google Scholar]
  13. Pringle J.R., Hartwell L.H. 1981; The Saccharomyces cererisiae cell cycle. In The Molecular Biology of the Yeast Saccharomyces. Life Cycle and Inheritance pp. 97–142 Strathern J.N., Jones E.W., Broach I.R. Edited by Cold Spring Harbor,New York: Cold Spring Harbor LaboraIory;
    [Google Scholar]
  14. Smith J.A, Martin L. 1973; Do cells cycle?. Proceedings of the National Academy of Sciences of the United States of Anwrica 70:1263–1267
    [Google Scholar]
  15. Sudberp P.E., Goodey A.R., Carterb L.A. 1980; Genes which control cell proliferation in the yeast Succhuromjrus ceruiisiae. Nuture; London:401–404
    [Google Scholar]
  16. Thuriaux P., Nurse P., Carter B. 1978; Mutants altered in the control coordinating cell division with cell growth in the fission yeast Schizosacchuromyces pombe. Molecular and General Genetics 161:215–220
    [Google Scholar]
  17. Tyson C.B., Lord P.G., Wheals A.E. 1979; Dependency of size of Saccharomyces cererisiae cells on growth rate. Journal of Bacteriology 138:92–94
    [Google Scholar]
  18. Wheals A.E. 1982; Size control models of Saccharomyces curerisiar ccll proliferation. Moluculor ond Cellulur Biology 2:361–368
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-11-2803
Loading
/content/journal/micro/10.1099/00221287-130-11-2803
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error