1887

Abstract

The mycolic acids of 15 cultures of representative substrains were examined by alkaline hydrolysis followed by esterification and thin-layer chromatography of the resulting methyl esters. Representatives of the Moreau and Swedish substrains had mycolic acid patterns similar to , composed of α-mycolates, methoxymycolates and ketomycolates, but examples of the Pasteur, Glaxo, Prague, Danish and Chinese substrains did not contain methoxymycolates. This apparent subdivision of substrains is in accordance with their varying antigenic patterns recorded in other studies.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-10-2733
1984-10-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/10/mic-130-10-2733.html?itemId=/content/journal/micro/10.1099/00221287-130-10-2733&mimeType=html&fmt=ahah

References

  1. Adam A., Senn M., Vilkas E., Lederer E. 1967; Spectrometrie de masse de glycolipides. 2. Diesters de trehalose naturels et synthetiques. European Journal of Biochemistry 2:460–468
    [Google Scholar]
  2. Asselineau J., Portelance V. 1974; Comparative study of the free lipids of eight BCG daughter strains. Recent Results in Cancer Research 47:214–220
    [Google Scholar]
  3. DaffÉ M., LanÉelle M.A., Asselineau C., LÉvy-FrÉbault V., David H. 1983; Intérêt taxonomique des acides gras des mycobacteries: proposition d’une méthode d’analyse. Annates de Microbiologie 134B:241–256
    [Google Scholar]
  4. Dobson G., Minnikin D.E., Parlett J.H., Goodfellow M., Ridell M., Magnusson M. 1984; Systematic analysis of complex myco-bacterial lipids. In Chemical Methods in Bacterial Systematics. Goodfellow M., Minnikin D. E. Edited by London: Academic Press (in the Press);
    [Google Scholar]
  5. Harboe M., Nagai S. 1984; MPB70, a unique antigen of Mycobacterium bovis BCG. American Review of Respiratory Diseases 129:444–452
    [Google Scholar]
  6. Jespersen A. 1971 The potency of BCG vaccines determined on animals. PhD thesis. University of Copenhagen.:
    [Google Scholar]
  7. Lind A. 1960; Serological studies of mycobacteria by means of diffusion-in-gel techniques. A difference in precipitinogenic content found in substrains of BCG. International Archives of Allergy and Applied Immunology 17:1–9
    [Google Scholar]
  8. Meissner G. 1968; Kulturelle Artendifferenzierung. In Mykobakterien und mykobakterielle Krankheiten. Teil IV. Laboratoriumsdiagnose der Mykobakterien pp. 149–237 Meissner G., Schmeidel A. Edited by Jena: Gustav Fischer;
    [Google Scholar]
  9. Minnikin D.E., Goodfellow M. 1982; Lipids: complex lipids, their chemistry, biosynthesis and roles. In The Biology of the Mycobacteria 1 pp. 95–184 Ratledge C., Stanford J.L. Edited by London:: Academic Press.;
    [Google Scholar]
  10. Minnikin D.E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid-fast bacteria. In Microbiological Classification and Identification pp. 189–256 Goodfellow M., Board R.G. Edited by London:: Academic Press.;
    [Google Scholar]
  11. Minnikin D.E., Hutchinson I.G., Caldicott A.B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. Journal of Chromatography 188:221–233
    [Google Scholar]
  12. Minnikin D.E., Minnikin S.M., Dobson G., Goodfellow M., Portaels F., Van Den Breen L., Sesardic D. 1983; Mycolic acid patterns of four vaccine strains of Mycobacterium bovis BCG. Journal of General Microbiology 129:889–891
    [Google Scholar]
  13. Miura K., Nagai S., Kinomoto M., Haga S., Tokunaga T. 1983; Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein. MPB70. Infection and Immunity 39:540–545
    [Google Scholar]
  14. Rosenthal S.R. 1980 BCG Vaccine: Tuberculosis-Cancer. Littleton, Massachusetts:: PSG Publishing Company.;
    [Google Scholar]
  15. Stavri D., Niculescu D., Stavri H., Popa V. 1981; Antigenic composition of different BCG substrains studied by rocket-line immuno-electrophoresis. Archives of Roumanian Pathology and Experimental Microbiology 40:113–116
    [Google Scholar]
  16. Strain S.M., Toubiana R., Ribi E., Parker R. 1977; Separation of the mixture of trehalose 6,6-dimycolates comprising the mycobacterial glycotipid fraction, ‘P3’. Biochemical and Biophysical Research Communications 77:449–456
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-10-2733
Loading
/content/journal/micro/10.1099/00221287-130-10-2733
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error