1887

Abstract

Several lytic and lysogenic actinophages were isolated from soil samples infected with and The morphologies and some biological properties of the phages, and the physico-chemical characteristics of their , were compared. Electron micrographs indicated that all the phage heads were of an icosahedral form, but head size and length of the tail varied. Two of the phages had a broad host range; the other isolates could lyse only a limited number of species. The molecular sizes of the phage were between 32·2 and 98·5 kb as estimated by electron microscopy and restriction enzyme analysis. The same study also indicated that one of the species contained cohesive ends. The G + C content of the ranged between 45·1 and 74·2 mol % as estimated from melting studies. Sedimentation velocity experiments implied that several of the phage were probably heavily glycosylated or methylated. These modifications might explain the partial or slow digestion of some of the by several of the 23 restriction enzymes tested. Protoplasts of the appropriate strains could be efficiently transfected with phage in the presence of 25% (w/v) polyethylene glycol (mol. wt 6000).

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1984-10-01
2022-01-22
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References

  1. Bradley S.R., Anderson U.L., Jones L.A. 1961; Phylogeny of Actinomycetes as revealed by susceptibility to actinophage. Developments in Industrial Microbiology 2:223–237
    [Google Scholar]
  2. Burkardt H.J., Mattes R., Pühler A., Heu-Mann W. 1978; Electron microscopy and computerized evaluation of some partially denatured group P resistance plasmids. Journal of General Microbiology 105:51–62
    [Google Scholar]
  3. Chater K.F. 1980; Actinophage DNA. Developments in Industrial Microbiology 21:65–74
    [Google Scholar]
  4. Chater K.F., Hopwood D.a., Kieser T., Thompson C.J. 1982; Gene cloning in Streptomy- cetes. Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  5. Chung S.T. 1982; Isolation and characterization of Streptomyces fradiae plasmids which are prophage of the actinophage ɸSF1. Gene 17:239–246
    [Google Scholar]
  6. Cross T., Goodfellow M. 1973; Taxonomy and classification of the actinomycetes. In Actinomyce-tales pp. 11–112 Sykes G., Skinner F.A. Edited by London & New York: Academic Press;
    [Google Scholar]
  7. Hirochika H., Sakaguchi K. 1982; Analysis of linear plasmids isolated from Streptomyces: association of protein with the ends of the plasmid DNA. Plasmid 7:59–65
    [Google Scholar]
  8. Huang L.-H., Farnet C.M., Ehrlich C., Ehrlich M. 1982; Digestion of highly modified bacteriophage DNA by restriction endonucleases. Nucleic Acids Research 10:1579–1591
    [Google Scholar]
  9. Isogai T., Takahashi H., Saito H. 1980; High- frequency protoplast transfection of Streptomyces parvulus 2297 with actinophage R4 DNA. Agricultural and Biological Chemistry 44:2425–2428
    [Google Scholar]
  10. Korn F., Weingärtner B., Kutzner H.J. 1978; A study of twenty actinophages: morphology, serological relationship and host range. In Genetics of the Actinomycetales pp. 251–270 Freerksen E., Tamok I., Thumin J.H. Edited by Stuttgart & New York: Fisher G.;
    [Google Scholar]
  11. Krügel H., Fiedler G., Noack D. 1980; Transfection of protoplasts from Streptomyces liri- dans 66 with actinophage SH 10 DNA. Molecular and General Genetics 177:297–300
    [Google Scholar]
  12. Lomovskaya N.D., Chater K.F., Mkrtumian N. 1980; Genetics and molecular biology of Streptomyces phages. Microbiological Reviews 44:206–229
    [Google Scholar]
  13. Lomovskaya N.D., Sladkova I.A., Klochkova O.A., Chinenova T.A., Mkrtumian N.M., Orekhov A.V. 1982; Systems for cloning and transfer in Streptomyces. In Overproduction of Microbial Products pp. 395–403 Krum phanzl V., Sikyta B., Vanek Z. Edited by London & New York: Academic Press;
    [Google Scholar]
  14. Mandel M.A., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology 12B:195–206
    [Google Scholar]
  15. Marmur J., Dotty P. 1962; Determination of base composition of deoxyribonucleic acid from thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  16. Miller J.H. 1972 Experiments in Molecular Genetics. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Okanishi M., Suzuki K., Umezawa H. 1974; Formation and reversion of streptomycete protoplasts: cultural conditions and morphological study. Journal of General Microbiology 81:389–400
    [Google Scholar]
  18. Pühler A., Burkardt H.J., Cannon F.C., Wohlleben W. 1979; Spontaneous degradation of pRDl DNA into unique size classes is recA dependent. Molecular and General Genetics 171:1–6
    [Google Scholar]
  19. Stuttard C., Dwyer M. 1981; A new temperate phage of Streptomyces venezuelae: morphology, DNA molecular-weight, and host range of SV2. Canadian Journal of Microbiology 27:496–499
    [Google Scholar]
  20. Suarez J.E., Chater K.F. 1980; Polyethylene glycol-assisted transfection of Streptomyces protoplasts. Journal of Bacteriology 142:8–14
    [Google Scholar]
  21. Szybalski W., Szybalski E.H. 1977; Equilibrium density gradient centrifugation. In Procedures in Nucleic Acid Research 2 pp. 311–354 Cantoni G.L., Davies D.R. Edited by New York: Harper & Row;
    [Google Scholar]
  22. Valentine R.C., Shapiro B.M., Stadtmann E.R. 1968; Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry 7:2143–2152
    [Google Scholar]
  23. Wohlleben W., Pühler A. 1982; Isolation and characterization of a plasmid in Streptomyces ghan- aensis. In 4th International Symposium on the Genetics of Industrial Microorganisms. Kyoto, Japan: Abstract Book. p. 122
    [Google Scholar]
  24. Woodward R.S., Lebowitz J. 1980; A revised equation relating DNA buoyant density to guano- sine plus cytosine content. Journal of Biochemical and Biophysical Methods 2:307–309
    [Google Scholar]
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