SUMMARY: Mutants of deficient in adenine phosphoribosyltransferase (A-PRT, EC have been isolated following selection for resistance to 8-azaadenine in a prototrophic strain carrying the allele of the gene coding for amidophosphoribosyltransferase(EC The mutants were recessive and defined a single gene, They did not excrete purine when combined with The mutants appeared to retain some A-PRT activity in crude extracts, and strains of the genotype responded to both adenine and hypoxanthine. Mutants deficient in adenine aminohydrolase (EC activity, , and hypoxanthine: guanine phosphoribosyltransferase (EC activity, , were used to synthesize the genotypes and The absence of A-PRT activity in strains with these genotypes confirmed the hypothesis that the residual A-PRT activity mutants was due to adenine aminohydrolase and hypoxanthine:guanine phosphoribosyltransferase acting in concert.


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