SUMMARY: Isolates of the plant pathogenic fungus can demethylate pisatin, an isoflavonoid phytoalexin produced by pea. Previous evidence has shown that pisatin demethylase is a cytochrome P-450 monooxygenase. In culture some isolates can be induced to demethylate pisatin at a high rate by pretreatment with the substrate, while others demethylate pisatin more slowly, regardless of pretreatment. Results from the present study indicate that in two naturally occurring isolates these pisatin demethylating phenotypes are controlled by genes at three loci. The phenotype with high pisatin demethylating activity is conferred by an allele at locus Alleles at each of two other loci confer low pisatin demethylating activity. Only isolates with negative alleles at all three loci lack pisatin demethylase activity altogether. Locus appears to be linked to a gene determining female fertility in this fungus.


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