1887

Abstract

Isolates of the plant pathogenic fungus can demethylate pisatin, an isoflavonoid phytoalexin produced by pea. Previous evidence has shown that pisatin demethylase is a cytochrome P-450 monooxygenase. In culture some isolates can be induced to demethylate pisatin at a high rate by pretreatment with the substrate, while others demethylate pisatin more slowly, regardless of pretreatment. Results from the present study indicate that in two naturally occurring isolates these pisatin demethylating phenotypes are controlled by genes at three loci. The phenotype with high pisatin demethylating activity is conferred by an allele at locus Alleles at each of two other loci confer low pisatin demethylating activity. Only isolates with negative alleles at all three loci lack pisatin demethylase activity altogether. Locus appears to be linked to a gene determining female fertility in this fungus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-10-2595
1984-10-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/10/mic-130-10-2595.html?itemId=/content/journal/micro/10.1099/00221287-130-10-2595&mimeType=html&fmt=ahah

References

  1. Cruickshank I.A.M. 1980; Defenses triggered by the invader: Chemical defenses. In Plant Disease: An Advanced Treatise V: pp. 247–267 Horsfall J.G., Cowling E.B. Edited by New York & London: Academic Press;
    [Google Scholar]
  2. Dbfago G., Kern H., Sedlar L. 1983; Genetic analysis of tomatine insensitivity, sterol content and pathogenicity for green tomato fruits in mutants of Fusarium solani. Physiological Plant Pathology 22:39–43
    [Google Scholar]
  3. Desjardins A.E., Matthews D.E., VanEtten H.D. 1984; Solubilization and reconstitution of pisatin demethylase, a cytochrome P-450 from the pathogenic fungus Nectria haematococca. Plant Physiology (in the Press)
    [Google Scholar]
  4. Dimock A.W. 1937; Hybridization studies on a zinc- induced variant of Hypomyces ipomoeae. Mycologia 29:273–285
    [Google Scholar]
  5. Guengerich F.P., Dannan G.A., Wright S.T., Martin M.V., Kaminsky L.S. 1982; Purification and characterization of liver microsomal cytochromes P-450: Electrophoretic, spectral, catalytic, and immunochemical properties and inducibility of eight isozymes isolated from rats treated with pheno- barbital or β-Naphthoflavone. Biochemistry 21:6019–6030
    [Google Scholar]
  6. Holenstein J., Defago G. 1983; Inheritance of naphthazarin production and pathogenicity to pea in Nectria haematococca. Journal of Experimental Botany 34:927–935
    [Google Scholar]
  7. Kathariou S., Spieth P.T. 1982; Spore killer polymorphism in Fusarium moniliforme. Genetics 102:19–24
    [Google Scholar]
  8. Kistler H.C. 1983 Phytoalexin metabolism and implications for phytoalexin tolerance and virulence in two plant pathogenic fimgi. PhD thesis Cornell University: Ithaca, NY, USA.;
    [Google Scholar]
  9. Kistler H.C., VanEtten H.D. 1984; Regulation of pisatin demethylation in Nectria haematococca and its influence on pisatin tolerance and virulence. Journal of General Microbiology 1302605–2613
    [Google Scholar]
  10. Lang M.A., Gielen J.E., Nebert D.W. 1981; Genetic evidence for many unique liver microsomal P-450-mediated monooxygenase activities in hetero- geneic stock mice. Journal of Biological Chemistry 296:12068–12075
    [Google Scholar]
  11. Matthews D.E., Vanetten H.D. 1983; Detoxification of the phytoalexin pisatin by a fungal cytochrome P-450. Archives of Biochemistry and Biophysics 224:494–505
    [Google Scholar]
  12. Negishi M., Nebert D.W. 1979; Structural gene products of the Ah locus. Journal of Biological Chemistry 254:11015–11023
    [Google Scholar]
  13. Perrin D.R., Bottomley W. 1962; Studies on phytoalexins. V. The structure of pisatin from Pisum sativum L. Journal of the American Chemical Society 84:1919–1922
    [Google Scholar]
  14. Sato R., Aoyama T., Imaj Y. 1982; Multiple forms of cytochrome P-430 from liver microsomes of drug untreated rabbits: Purification and characterization. In Oxygenases and Oxygen Metabolism pp. 321–332 Nozaki M. Edited by New York: Academic Press;
    [Google Scholar]
  15. Stevens R.B.editor 1974 Mycology Handbook. Seattle & London: University of Washington Press;
    [Google Scholar]
  16. Tegtmeier K.J., Vanetten H.D. 1982a; Genetic studies on selected traits of Nectria haematococca. Phytopathology 72:604–607
    [Google Scholar]
  17. Tegtmeier K.J., VanEttbn H.D. 1982b; The role of pisatin tolerance and degradation in the virulence of Nectria haematococca on peas: A genetic analysis. Phytopathology 72:608–612
    [Google Scholar]
  18. Tukey R.H., Negishi M., Nebert D.W. 1982; Quantitation of hepatic cytochrome P,1-450 mRNA with the use of a cloned DNA probe. Molecular Pharmacology 22:779–786
    [Google Scholar]
  19. Turner B.C., Perkins D.D. 1979; Spore killer, a chromosomal factor in Neurospora that kills meiotic products not containing it. Genetics 93:587–606
    [Google Scholar]
  20. VanEtten H.D. 1973; Differential sensitivity of fungi to pisatin and to phaseolin. Phytopathology 63:1477–1482
    [Google Scholar]
  21. VanEtten H.D. 1978; Identification of additional habitats of Nectria haematococca mating population VI. Phytopathology 68:1552–1556
    [Google Scholar]
  22. VanEtten H.D. 1982; Phytoalexin detoxification by monooxygenases and its importance for pathogenicity. In Plant Infection: The Physiological and Biochemical Basis pp. 315–327 Asada Y., Bushnell W.R., Ouchi S., Vance C.P. Edited by Tokyo/Springer-Verlag; Berlin: Japan Sci. Soc. Press:
    [Google Scholar]
  23. VanEtten H.D., Matthews P.S. 1984; Naturally-occurring variation in the induction of pisatin demethylating ability in Nectria haematococca mating population VI. Physiological Plant Pathology (in the Press)
    [Google Scholar]
  24. VanEtten H.D., Pueppkb S.G., Kelsey T.C. 1975; 3,6a-Dihydroxy-8,9-methylenedioxyptero- carpan as a metabolite of pisatin produced by Fusarium solani f. sp. pisi. Phytochemistry 14:1103–1105
    [Google Scholar]
  25. VanEtten H.D., Matthews P.S., Tegtmeier K.J., Dibtert M.F., Stein J.I. 1980; The association of pisatin tolerance and demethylation with virulence on pea in Nectria haematococca. Physiological Plant Pathology 16:257–268
    [Google Scholar]
  26. VanEtten H.D., Matthews D.E., Smith D.A. 1982; Metabolism of Phytoalexins. In Phytoalexins. pp. 181–217 Bailey J.A., Mansfield J.W. Edited by London: Blackie;
    [Google Scholar]
  27. Yoder K.S., Klos E.J., Nowacka H., Biblenin A. 1982; Inheritance of an ascospore abortion factor in Venturia inaequalis. Canadian Journal of Botany 60:2105–2111
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-10-2595
Loading
/content/journal/micro/10.1099/00221287-130-10-2595
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error