Effect of Nitrogen and Phosphate on the Levels of Intermediates in Bakers’ Yeast Grown in Continuous Culture Free

Abstract

Bakers’ yeast () has been grown in continuous culture using a control medium and media which contained low levels of ammonium and phosphate. The effects of medium composition and growth rate on the levels of intermediates of the glycolytic pathways, the tricarboxylic acid cycle and the glyoxylate cycle were investigated. The energy charge varied only between 0·7 and 0·9 over the range of dilution rates studied; however, the level of ATP decreased by 50% at higher aerobic growth rates. Intermediates of the Embden-Meyerhof-Parnas pathway were higher at the low aerobic growth rates and decreased as the dilution rate was increased. However, higher levels of these intermediates were also observed at even higher dilution rates at which ethanol formation and fermentative metabolism occurred. Significant differences in levels of intermediates were observed between control experiments and fermentations using the low nitrogen and phosphate media. The greatest differences were observed in the levels of glucose 6-phosphate, 6-phosphogluconate, pyruvate, citrate and glyoxylate. Twenty-one different steady states were investigated and each was found to have a unique composition.

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1984-10-01
2024-03-29
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References

  1. Ahmad F.A., Rose A.H., Garg N.K. 1961; F.ffect of biotin deficiency on the synthesis of nucleic acids and proteins by S. cerevisiae. Journal of General Microbiology 24:69–80
    [Google Scholar]
  2. Atkisson D.F. 1977 Cellular Energy Metabolism. New York: Academic Press;
    [Google Scholar]
  3. Atkissos D.F., Walton G.M. 1967; Adenosine triphosphate conservation on metabolic regulation. Journal of Biological Chemistry 242:3239–3341
    [Google Scholar]
  4. Ball W.J., Atkisson D.E. 1975; Adenylate charge in Saccharomyces cerevisiae during starvation. Journal of Bacteriology 121:975–982
    [Google Scholar]
  5. Barford J.P., Hall R.J. 1979; An examination of the Crabtree effect in Saccharomyces cerevisiae the role of respiratory adaptation. Journal of General Microbiology 114:267–275
    [Google Scholar]
  6. Bergmeyer H.U, Bernt F. 1974; Determination with glucose oxidase and peroxidase. In Methods of Enzymatic Analysis 3 pp. 1205–1215 Bergmeyer H.U. New York: Academic Press;
    [Google Scholar]
  7. Bernt E., Gutman I. 1974; Determination with alcohol dehydrogenase and NAD. In Methods of Enzymatic Analysis 3: pp. 1499–1502 Bergmeyer H.U. Edited by New York: Academic Press;
    [Google Scholar]
  8. Berry D.R., Smith J.F., Franco C.M.M. 1981; Intermediate levels in bakers yeast grown in continuous culture. In Current Developments m Yeast Research pp. 393–398 Stewart G.G., Russell I. Edited by Toronto: Pergamon;
    [Google Scholar]
  9. Burrows S. 1970; Bakers Yeast. In The Yeasts 3 pp. 349–420 Rose A.H., Harrison J.S. Edited by London: New York: Academic Press;
    [Google Scholar]
  10. Blrrows S. 1979; Bakers Yeast. In Economic Microbiology 4 pp. 31–64 Rose A.H. Edited by London & New York: Academic Press;
    [Google Scholar]
  11. Chamberlain N., Cutts N.S., Rainbow C. 1954; The formation of pigment and arylamine by yeasts. Journal of General Microbiology 7:54–60
    [Google Scholar]
  12. Chapaman A.G., Atkinson D.E. 1977; Adenine nucleotide concentrations and turnover rates, their correlation with biological activity in bacteria and yeast. Advances in Microbial Physiology 15:253–306
    [Google Scholar]
  13. Eastbrook R.W., Maitra P.K. 1964; A fluorimetric method for the quantitative microanalysis of adenine pyridine nucleotides. Analytical Biochemistry 3:369–382
    [Google Scholar]
  14. Fiechter O., Flhrmann G.F., Kappeli O. 1981; Regulation of glucose metabolism in growing yeast cells. Advances in Microbial Physiology 22:123–183
    [Google Scholar]
  15. Forrest W.W. 1965; ATP pool during the growth cycle of Streptococcus faecalis. Journal of Bacteriology 90:1013–1016
    [Google Scholar]
  16. Franco C.M.M. 1980 Studies on intermediary metabolism of bakers yeast during aerobic growth. PhD thesis. University of Strathclyde;
    [Google Scholar]
  17. Harder W., Dijkhltzen L. 1983; Physiological responses to nutrient limitation. Annual Review of Microbiology 37:1–23
    [Google Scholar]
  18. Kuenzi M.T., Fiechter A. 1969; Changes in carbohydrate composition and trehalose during the budding cycle of Saccharomyces cerevisiae. Archives of Microbiology 64:396–407
    [Google Scholar]
  19. Kuenzi M.T., Fiechter A. 1972; Regulation of carbohydrate composition of Saccharomyces cerevisiae undergrowth limitation. Archives of Microbiology 84:254–262
    [Google Scholar]
  20. Lundin A., Thore A. 1975; Comparison of methods for extraction of bacterial adenine nucleotides determined by firefly assay. Applied Microbiology 30:713–721
    [Google Scholar]
  21. Mian F.A., Fencl S., Prokop A., Monagegeghi A. 1974; Effect of growth rate on glucose metabolism of yeast grown in continuous culture. Folia microbiologica 19:191–198
    [Google Scholar]
  22. Neijssel O.M., Tempest D.W. 1976; The role of carbohydrate metabolism in Klebsiella aerogenes NCTC 418 organism growing in a chemostat culture. Archives of Microbiology 106:251–258
    [Google Scholar]
  23. Oura E. 1974; Effect of aeration intensity on the biochemical composition of bakers yeast. I. Factors affecting the type of metabolism. Biotechnology and Bioengineering 16:1197–1212
    [Google Scholar]
  24. Pirt S.J. 1975 Principles of Microbe and Celt Cultivation. London: Blackwell Scientific;
    [Google Scholar]
  25. Sols A., Gancedo C., Delafuente G. 1971; Energy yielding metabolism in yeast. In The Yeasts 2 pp. 271–367 Rose A.H., Harrison J.S. Edited by London & New York: Academic Press;
    [Google Scholar]
  26. Wales D.S., Cartledge T.G., Lloyd D. 1980; The effect of glucose repression and anaerobiosis on the activities and subcellular distribution of TCA cycles and associated enzymes in Saccharomyces carlsbergensis. Journal of General Microbiology 116:93–98
    [Google Scholar]
  27. Weibel K.E., Mor J.R., Fiechter A. 1974; Rapid sampling of yeast cells and automated assays of adenylate, citrate, pyruvate and glucose 6- phosphate pools. Analytical Biochemistry 58:208–216
    [Google Scholar]
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