1887

Abstract

SUMMARY: The influence of Na and K on the synthesis and secretion of extracellular glucosyltransferase (GTF; EC 2.4.1.5) and fructosyltransferase (FTF; EC 2.4.1.10) by NCTC 7865 and Challis NCTC 7868 has been determined. No FTF and little or no mutansucrase (GTF-I) activities were detectable during growth on glucose or sucrose unless the Na/K ratio of the cultures was kept low. Increasing K concentrations stimulated the production of FTF and dextransucrase (GTF-S), but all glycosyltransferase activities decreased in high K media when the growth pH was maintained with NaOH instead of KOH, indicating that the Na/K ratio effect was due principally to Na inhibition. Significant GTF and FTF activities were detected in a putative GTF mutant of strain Challis grown in high K medium but not in high Na medium, suggesting that the mutant might be defective in a regulatory gene.

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/content/journal/micro/10.1099/00221287-130-1-77
1984-01-01
2019-10-16
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-1-77
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