SUMMARY: The major phospholipids of the anaerobic rumen phycomycete were phosphatidylethanolamine (38%), phosphatidylcholine (26%) and phosphatidylinositol (13%); no sphingolipids, glycolipids, plasmalogens or phosphonyl lipids were detected. Free fatty acids, triacylglycerols, 1:2 diacylglycerols and a variable amount of 1:3 diacylglycerol were identified, as were minor amounts of squalene and a triterpenol which is probably tetrahymanol. Approximately half the fatty acids were straight chain, even 12 to 24 carbon, saturated acids, the remainder being even 16 to 24 carbon, mono-unsaturated fatty acids. The double bonds in all except the 16 carbon acid were in the ω9 position. The unsaturation is introduced by a δ9 desaturase which uses stearic acid as substrate and which does not use oxygen as a terminal electron acceptor. C from acetate and glucose was incorporated into the fatty acids of all complex lipids, as were lauric, myristic, palmitic, stearic and oleic acids. [C]Choline was incorporated into phosphatidylcholine and [C]ethanolamine into phosphatidylethanolamine and phosphatidylcholine. Label from [C]serine was recovered in phosphatidylserine and phosphatidylethanolamine, but was not detected in phosphatidylcholine.


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