1887

Abstract

The insertion pattern of flagellation of was determined from electron micrographs of negatively stained cells treated with various detergents to remove their outer cell wall layer(s) selectively. The best results were obtained with 0·2% sodium dodecyl sulphate and a treatment time of 1–2 min. Detergent-treated cells displayed a unique helical pattern of flagellation which we designate as helicotrichous (Gk n. , helix + Gk n. , hair). Flagella originated from an electron-dense region approximately 180 nm wide, which spiralled around the cell body. The length of the insertion path averaged 2·6 μm per 360° turn; its periodicity was 2·5 μm. An average of 19 flagella were inserted per 1 μm length of the electrondense region. The effects of cell division and branching on flagellation were also examined.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-1-185
1984-01-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/1/mic-130-1-185.html?itemId=/content/journal/micro/10.1099/00221287-130-1-185&mimeType=html&fmt=ahah

References

  1. Adam G. 1977; Rotation of bacterial flagella as driven by cytomembrane streaming. Journal of Theoretical Biology 65:713–726
    [Google Scholar]
  2. Anderson R. A. 1975; Formation of the bacterial flagellar bundle. In Swimming and Fixing in Nature I pp. 45–56 Edited by Wu T. Y. T., Brokaw C. J., Brennen C. New York: Plenum Press;
    [Google Scholar]
  3. Bayer M. E., Anderson T. F. 1965; The surface structure of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 54:1592–1599
    [Google Scholar]
  4. Berg H. C. 1974; Dynamic properties of bacterial flagellar motors. Nature, London 249:77–79
    [Google Scholar]
  5. Berg H. C. 1976; How spirochetes may swim. Journal of Theoretical Biology 56:269–273
    [Google Scholar]
  6. Berg H. C., Manson M. D., Conley M. P. 1982; Dynamics and energetics of flagellar rotation in bacteria. Symposia of the Society for Experimental Biology 35:1–31
    [Google Scholar]
  7. Cohen-Bazire G., London J. 1967; Basal organelles of bacterial flagella. Journal of Bacteriology 94:458–465
    [Google Scholar]
  8. Coulton J. W., Murray R. G. E. 1978; Cell envelope associations of Aquaspirillum serpens flagella. Journal of Bacteriology 136:1037–1049
    [Google Scholar]
  9. Depamphilis M. L., Adler J. 1971a; Fine structure and isolation of the hook basal body complex of flagella from Escherichia coli and Bacillus suhtilis. Journal of Bacteriology 105:384–395
    [Google Scholar]
  10. Depamphilis M. L., Adler J. 1971b; Attachment of flagellar basal bodies to the cell envelope : specific attachment to the outer, lipopolysaccharide membrane and the cytoplasmic membrane. Journal of Bacteriology 105:396–407
    [Google Scholar]
  11. Hill M. J. 1967; Action of bile salts on bacterial cell walls. Nature, London 214:1152–1154
    [Google Scholar]
  12. Holdeman L. V., Cato E. P, Moore W. E. C. editors 1977 Anaerobe Laboratory Manual, 4th edn. p. 144 Blacksburg: Virginia Polytechnic Institute Anaerobe Laboratory;
    [Google Scholar]
  13. Hougen K. H., Birch-Andersen A. 1971; Electron microscopy of endoflagella and microtubules in Treponema Reiter. Acta pathologica et microbiologica scandinavica, Section B 79:37–50
    [Google Scholar]
  14. Hylemon P. B., Wells J. S. JR Krieg N. R., Jannasch H. W. 1973; The genus Spirillum, a taxonomic study. International Journal of Systematic Bacteriology 23:340–380
    [Google Scholar]
  15. Kingsley V. V., Hoeniger J. F. M. 1973; Growth, structure and classification of Selenomonas. Bacteriological Reviews 37:479–521
    [Google Scholar]
  16. Lai C.-H., Dougherty P., Listgarten M. A. 1981a; Partial characterization of unidentified gram-negative, anaerobic, motile rods from periodontitis lesions. Journal of Dental Research 60:331
    [Google Scholar]
  17. Lai C.-H., Listgarten M. A., Tanner A. C. R., Socransky S. S. 1981b; Ultrastructures of Bacteroidesgracilis, Campylobacter concisus, Wolinella recta, and Eikenellacorrodens, all from humans with periodontal disease. International Journal of Systematic Bacteriology 31:465–475
    [Google Scholar]
  18. Lai C.-H., Males B. M., Dougherty P. A., Berthold P., Listgarten M. A. 1983; Centipeda periodontii, gen. nov., sp. nov., from human periodontal lesions. International Journal of Systematic Bacteriology 33:628–635
    [Google Scholar]
  19. Lauger P. 1977; Ion transport and rotation of bacterial flagella. Nature, London 268:360–362
    [Google Scholar]
  20. Lee S. Y., Mabee M. S., Jangaard N. O. 1978; Pectinatus, a new genus of the family Bacteroidaceae. International Journal of Systematic Bacteriology 28:582–594
    [Google Scholar]
  21. Macnab R. M. 1977; Bacterial flagella rotating in bundles: a study in helical geometry. Proceedings of the National Academy of Sciences of the United States of America 74:221–225
    [Google Scholar]
  22. Macnab R. M. 1979; How do flagella propel bacteria?. Trends in Biomedical Science 4:N10–13
    [Google Scholar]
  23. Manson M. D., Tedesco P. M., Berg H. C. 1980; Energetics of flagellar rotation in bacteria. Journal of Molecular Biology 138:541–561
    [Google Scholar]
  24. Murray R. G. E., Birch-Andersen A. 1963; Specialized structure in the region of the flagella tuft in Spirillum serpens. Canadian Journal of Microbiology 9:393–401
    [Google Scholar]
  25. Remsen C. C., Watson S. W., Waterbury J. B., Truper H. G. 1968; Fine structure of Ectothiorho-dospiramobilis Pelsh. Journal of Bacteriology 95:2374–2392
    [Google Scholar]
  26. Ritchie A. E., Keeler R. F., Bryner J. H. 1966; Anatomical features of Vibrio fetus: electron microscopic survey. Journal of General Microbiology 43:427–438
    [Google Scholar]
  27. Vaituzis Z., Doetsch R. N. 1969; Relationship between cell wall, cytoplasmic membrane, and bacterial motility. Journal of Bacteriology 100:512–521
    [Google Scholar]
  28. Weibull C. 1960; Movement. In The Bacteria pp. 153–205 Edited by Gunsalus I. C., Stanier R. Y. New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-1-185
Loading
/content/journal/micro/10.1099/00221287-130-1-185
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error