1887

Abstract

The patterns of polypeptides synthesized by two co-isogenic monokaryons and the derived dikaryon of the basidiomycete , growing in surface culture, were compared by two-dimensional PAGE after labelling with [S]sulphate. Synthesis of most of the polypeptides (400 analysed) was common but at day 3 some 30 polypeptides were synthesized in the dikaryon which were not seen in the monokaryons. At this time the dikaryon had formed hyphal aggregates. Ten additional novel polypeptides were being synthesized in the primordia arising from these aggregates at day 4.

In surface culture, monokaryons and dikaryon excreted 4 to 8% of the labelled proteins (over 80 different polypeptides) into the medium compared with only 1 to 2% when cultivation was done in liquid shaken cultures. The polypeptides specifically secreted by the dikaryon at the aggregation stage consisted of seven small polypeptides with molecular weights ranging from 10 to 14 × 10, and two polypeptides with molecular weights 18 × 10 and 26 × 10, respectively. Such differences in excreted proteins between monokaryons and dikaryon were not observed when the mycelia were grown in liquid shaken cultures, indicating a relationship with fruit-body formation.

A comparison of labelled proteins in isolated hyphal walls revealed six polypeptides specifically associated with the walls of monokaryons and dikaryon irrespective of the cultivation method. Among the polypeptides newly appearing in the dikaryon at aggregation two were associated with the walls and co-migrated with the polypeptides of molecular weights 18 × 10 and 26 × 10 excreted into the medium at this stage of development.

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1984-01-01
2024-10-09
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References

  1. Bull A. T., Trinci A. P. J. 1977; The physiology and metabolic control of fungal growth. Advances in Microbial Physiology 50:1–84
    [Google Scholar]
  2. Bu’Lock J. D. 1967; Fungal metabolites with structural function. In Essays in Biosynthesis and Microbial Development, E. R. Squibb Lectures on Chemistry of Microbial Products pp. 1–18 New York: Wiley;
    [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  4. Crandall M., Egel R., Mackay V. L. 1977; Physiology of mating in three yeasts. Advances in Microbial Physiology 15:307–398
    [Google Scholar]
  5. De Vries O. M. H., Hoge J. H. C., Wessels J. G. H. 1980a; Regulation of the pattern of protein synthesis inSchizophyllum commune. . Developmental Biology 74:22–36
    [Google Scholar]
  6. De Vries O. M. H., Hoge J. H. C., Wessels J. G. H. 1980b; Translation of RNA fromSchizophyllum commune in a wheat germ and rabbit reticulocyte cell-free system. Comparison of in vitro and in vivo products after two-dimensional gel electrophoresis. Biochimica et biophysica acta 607:373–378
    [Google Scholar]
  7. Dons J. J. M., De Vries O. M. H., Wessels J. G. H. 1979; Characterization of the genome of the basidiomyceteSchizophyllum commune. . Biochimica et biophysica acta 563:100–112
    [Google Scholar]
  8. Esser K., Saleh F., Meinhardt F. 1979; Genetics of fruit-body production in higher basidiomycetes. II. Monokaryotic and dikaryotic fruiting inSchizophyllum commune. . Current Genetics 1:85–88
    [Google Scholar]
  9. Evers D. C., Ross I. K. 1983; Isozyme patterns and morphogenesis in higher basidiomycetes. Experimental Mycology 7:9–16
    [Google Scholar]
  10. Hoge J. H. C., Springer J., Zantinge B., Wessels J. G. H. 1982a; Absence of differences in polysomal RNAs from vegetative monokaryotic and dikaryotic cells of the fungusSchizophyllum commune. . Experimental Mycology 6:225–232
    [Google Scholar]
  11. Hoge J. H. C., Springer J., Wessels J. G. H. 1982b; Changes in complex RNA during fruit- body initiation in the fungusSchizophyllum commune. . Experimental Mycology 6:233–243
    [Google Scholar]
  12. Laskey R. A., Mills A. D. 1975; Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  13. Leatham G. F., Stahmann M. A. 1981; Studies on the laccase ofLentinus edodes: specificity, localization and association with the development of fruiting-bodies. Journal of General Microbiology 125:147–157
    [Google Scholar]
  14. Leonard T. J., Phillips L. E. 1973; Study of phenoloxidase activity during the reproductive cycle inSchizophyllum commune. . Journal of Bacteriology 114:7–10
    [Google Scholar]
  15. Leslie J. F., Leonard T. J. 1979; Three independent genetic systems that control initiation of a fungal fruit-body. Molecular and General Genetics 171:257–260
    [Google Scholar]
  16. O’Farrell P. H. 1975; High resolution two-dimensional electophoresis of proteins. Journal of Biological Chemistry 10:4007–4021
    [Google Scholar]
  17. Pringle J. R. 1975; Methods for avoiding proteolytic artefacts in studies of enzymes and other proteins from yeasts. Methods in Cell Biology 12:149–184
    [Google Scholar]
  18. Schwalb M. N. 1978; Regulation of fruiting. In Genetics and Morphogenesis in the Basidiomycetes pp. 135–165 Edited by Schwalb M. N., Miles P. G. New York: Academic Press;
    [Google Scholar]
  19. Van Der Valk P., Marchant R. 1978; Hyphal ultrastructure in fruit-body primordia of the basidiomycetesSchizophyllum commune andCoprinus cinereus. . Protoplasma 95:57–72
    [Google Scholar]
  20. Volz P. A., Niederpruem D. J. 1970; Induced cytological aberrancies in basidiocarp morphology ofSchizophyllum commune. . Archives of Microbiology 72:371–374
    [Google Scholar]
  21. Wessels J. G. H. 1965; Morphogenesis and biochemical processes inSchizophyllum commune Fr. Wentia 13:1–113
    [Google Scholar]
  22. Wray W., Boulikas T., Wray V. P., Hancock R. 1981; Silver staining of proteins in polyacrylamide gels. Analytical Biochemistry 118:197–203
    [Google Scholar]
  23. Zantinge B., Dons H., Wessels J. G. H. 1979; Comparison of poly (A)-containing RNAs in different cell types of the lower eukaryoteSchizophyllum commune. . European Journal of Biochemistry 101:251–260
    [Google Scholar]
  24. Zantinge B., Hoge J. H. C., Wessels J. G. H. 1981; Frequency and diversity of RNA sequences in different cell types of the fungusSchizophyllum commune. . European Journal of Biochemistry 113:381–389
    [Google Scholar]
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