1887

Abstract

SUMMARY: A stable mutation in a strain of from penicillin sensitivity to penicillin resistance due to the production of large amounts of penicillinase is reported. By the velvet pad replicate technique this mutation has been shown to be a spontaneous event occurring in the absence of penicillin. Penicillin-resistant mutants were obtained and the penicillinase production proved, without ever bringing the cells into contact with the antibiotic. The mutation rate is about 3 × 10 mutations per bacterium per division.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-13-3-561
1955-12-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/13/3/mic-13-3-561.html?itemId=/content/journal/micro/10.1099/00221287-13-3-561&mimeType=html&fmt=ahah

References

  1. Armitage P. 1952; The statistical theory of bacterial populations subject to mutation. J. R. statist. Soc. B:14–1
    [Google Scholar]
  2. Atwood K.C., Schneider L.K., Ryan F.J. 1951; Periodic selection in Escherichia coli. Proc. nut. Acad. Sci., Wash 37:146
    [Google Scholar]
  3. Dean A.C.R., Hinshelwood C. 1952; The resistance of Bact. Zactis nerogenes to proflavine (2 : 8-diaminoacridine). 1. The applicability of the statistical fluctuation test. Proc. Roy. Soc B, 139:236
    [Google Scholar]
  4. Goldstein A. 1954; The origin of streptomycin-dependent variants of Escherichici coli. . J. Pharmacol. 112:326
    [Google Scholar]
  5. Henry R.J., Housewright R.D. 1947; Studies on penicillinase. II. Mano- metric method of assaying penicillinase and penicillin, kinetics of the penicillin- penicillinase reaction, and the effects of inhibitors on penicillinase. J. biol. Chem. 167:559
    [Google Scholar]
  6. Knight B.C.J.G., Proom H. 1950; A comparative survey of the nutrition and physiology of mesophilic species in the genus Badllus. . J. gen. Microbiol. 4:508
    [Google Scholar]
  7. Kraft M., Braun W. 1955; Observations on replica plating and antibiotic- resistance. Bact Proc. 52:
    [Google Scholar]
  8. Lam G.T., Sevag M.G. 1955; Mechanism of the development of resistance to streptomycin. II. Biochemical differences of replicate colonies. J. Bact 69:184
    [Google Scholar]
  9. Lea D.E., Coulson C.A. 1949; The distribution of the numbers of mutants in bacterial populations. J. Genet 49:264
    [Google Scholar]
  10. Lederberg J., Lederberg E.M. 1952; Replica plating and indirect selection of bacteriai mutants. J. Bact. 63:399
    [Google Scholar]
  11. Luria S.E., Delbrück M. 1943; Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491
    [Google Scholar]
  12. Manson E.E.D., Pollock M.R., Tridgell E.J. 1954; A comparison of the properties of penicillinase produced by Racing gaMttg and Racidag cereas with and without addition of penicillin. J. gen. Microbiol. 11:493
    [Google Scholar]
  13. Newcombe H.B. 1949; Origin of bacterial variants. Nature; Lond.: 164150
    [Google Scholar]
  14. Perret C.J. 1954; Iodometric assay of penicillinase. Nature; Lond.: 1741012
    [Google Scholar]
  15. Pollock M.R., Torriani A.-M., Tridgell E.J. 1956; Crystalline bacterial penicillinase. Biochem. J. in the Press
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-13-3-561
Loading
/content/journal/micro/10.1099/00221287-13-3-561
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error