Summary: The characteristic nutritional patterns were determined for seven species or varieties of the genus . Six strains of var. grew in a complex but defined amino acid medium + thiamine. Three strains of the insect pathogen var. had the same nutritional pattern as the parent species and grew in a seven amino acid medium. Of twenty strains of , ten grew on the seven amino acid medium + biotin while the growth of five other strains was markedly affected by the addition of thiamine. Two of the remaining five strains required thiamine on first isolation, but soon dispensed with it on subculture, while the other three strains continued to require thiamine. Six strains of grew in the seven amino acid medium + biotin + thiamine + urea. When the seven amino acid medium was replaced by the complex amino acid medium three of these strains of grew without the addition of urea, and the remaining three strains required either urea or ammonium chloride. The sixteen strains of all grew in the complex amino acid medium + biotin + thiamine. The three strains of grew in the seven amino acid medium + biotin.

The twenty-seven strains of formed a nutritionally heterogeneous group. Eight strains were maintained in serial subculture in broth but could not be grown in defined media. One strain grew in the ammonia basal medium alone, others required added biotin or added biotin + thiamine and others required amino acid media + biotin + thiamine.


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