Potentiation and Destruction of R Variant Coaǵulase by Products of S Variants of Free

Abstract

SUMMARY: Factors obtained from certain S variants of can both increase the potency of and destroy the coagulase produced by R variants of the organism. Evidence that separate factors are responsible for potentiation and for destruction of coagulase is based on differences of pH optimum and of temperature range of the factors. Potentiation of coagulase is most marked below pH 7 and occurs at temperatures ranging from 9° to 37°. The optimum pH value for destruction is between 7 and 8, and it does not occur at 9°. There is evidence, though incomplete, that both factors are enzymes. In the course of both potentiation and destruction the mode of clotting by R coagulase is altered from a granular to a solid type. Some properties of the factors and their partial purification are described and their possible mode of action is discussed.

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1955-12-01
2024-03-29
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References

  1. Biezunski N., Shafrir E., De Vries A., Katchalski E. 1955; The action of polylysine on the conversion of fibrinogen into fibrin by coagulase thrombin. Biochem. J. 59:55
    [Google Scholar]
  2. Edsall J.T., Lever W.F. 1951; Effects of ions and neutral molecules on fibrin clotting. J. biol. Chem. 191:735
    [Google Scholar]
  3. Elliott S.D. 1945; A proteolytic enzyme produced by group A streptococci, with special reference to its effect on the type-specific M antigen. J. exp. Med. 81:573
    [Google Scholar]
  4. Ferry J.D., Shulman S. 1949; The conversion of fibrinogen to fibrin. J. Amer. chem. Soc. 71:3198
    [Google Scholar]
  5. Fisk A. 1940; The technique of the coagulase test for staphylococci. Brit. J. exp. Path. 21:311
    [Google Scholar]
  6. Hale J.H., Smith W. 1945; The influence of coagulase on the phagocytosis of staphylococci. Brit. J. exp. Path. 26:209
    [Google Scholar]
  7. Hendry E.B. 1948; Iso-osmotic buffer solutions. Edinb. med. J. 55:142
    [Google Scholar]
  8. Kunitz M. 1945–6; Crystalline soybean trypsin inhibitor. J. gen. Physiol. 29:149
    [Google Scholar]
  9. Lominski I., Smith D.D., Morrison R.B. 1953; A coagulase destroying factor produced by variants of Staphylococcus aureus. . Nature; Lond.: 171214
    [Google Scholar]
  10. Smith D.D., Morrison R.B., Lominski I. 1952; Isolation of variants of Staphylococcus aureus giving high and low yields of coagulase. J. Path. Bact. 64:567
    [Google Scholar]
  11. Smith W., Hale J.H. 1944; The nature and mode of action of staphylococcus coagulase. Brit. J. exp. Path. 25:101
    [Google Scholar]
  12. Smith W., Hale J.H., Smith M.M. 1947; The role of coagulase in staphylococcal infections. Brit. J. exp. Path. 28:57
    [Google Scholar]
  13. Tager M. 1948a; Concentration, partial purification, properties and nature of staphyloeoagulase. Yale J. Biol. Med. 20:487
    [Google Scholar]
  14. Tager M. 1948b; Studies on the coagulase-reacting factor. Yale J. Biol. Med. 20:369
    [Google Scholar]
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