Summary: The behaviour of six strains of herpes simplex virus was studied. Marked differences were noted in the type of lesion formed and the rate of attachment when the strains were inoculated on to the chorio-allantois, and in the behaviour of strains inoculated intracerebrally into mice or on to the cornea of the rabbit.

Two easily differentiated strains were inoculated together on to the chorio-allantois under conditions designed to give rise to a proportion of doubly infected cells. After a short period of incubation the progeny of the first intracellular growth cycle was obtained.

Ten clones were isolated, of which six resembled one of the two parents in the type of lesion formed and level of virulence for mice. Of the other four, one (106 a) produced small type lesions and the other three large type. All possessed degrees of virulence intermediate between those of the parents. Three of these substrains showed no alteration in the type of lesions formed or their degrees of virulence after 3 to 5 passages of single lesions at limiting dilution. When the rate of attachment to the chorio-allantois and the behaviour when inoculated on to the cornea of the rabbit were studied, it was found that 106a produced effects intermediate between the parents, whilst the other strains resembled one of the two parents.


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