1887

Abstract

Summary: Spore suspensions of germinated spontaneously after heat treatment. The extent of germination depended on the degree of close packing of the suspension, the temperature and duration of heating, and on the presence of water. Spontaneous germination was not inhibited by heating with cyanide, azide, 2:4-dinitrophenol or sodium iodoacetate, but was significantly inhibited by sodium fluoride.

Freshly harvested spores of a laboratory strain of required either inosine or a mixture of alanine + tyrosine + adenosine for optimal germination. After prolonged storage or a short heat treatment, adenosine alone stimulated rapid and complete germination. Heat activation did not occur in the absence of water. It was inhibited to some extent by sodium fluoride. Similar results were obtained with , NCTC 8035.

After preheating, spores of a virulent and an avirulent strain of germinated more rapidly and completely in a mixture of adenosine + tyrosine + alanine, but not in adenosine alone. Inosine stimulated rapid and complete germination of heated suspensions, and was consistently more effective than adenosine. Adenosine deaminase activity has been found in extracts from resting spores of the laboratory and the avirulent strain of but not in .

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1955-08-01
2024-03-28
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References

  1. Curran H. R., Evans F. R. 1945; Heat activation inducing germination in the spores of thermotolerant and thermophilic aerobic bacteria. J. Bact 49:335
    [Google Scholar]
  2. Gladstone G. P., Fildes P. 1940; A simple culture medium for general use without meat extract or peptone. Brit. J. exp. Path 21:161
    [Google Scholar]
  3. Goddard D. R. 1939; The reversible heat activation of germination in Neurospora. Cold. Spr. Harh. Symp. quant. Biol 7:362
    [Google Scholar]
  4. Hills G. M. 1949; Chemical factors in the germination of spore-bearing aerobes. The effects of amino-acids on the germination of Bacillus anthracis with some observations on the relation of optical form to biological activity. Biochem. J 45:363
    [Google Scholar]
  5. Hull R. 1939; Study of Byssochlamys fulva and control measures in processed fruits. Ann. appl. Biol 26:800
    [Google Scholar]
  6. Mickle H. 1948; Tissue disintegrator. J. R. micr. Soc 68:10
    [Google Scholar]
  7. Powell J. F. 1950; Factors affecting the germination of thick suspensions of Bacillus subtilis spores in L-alanine solution. J. gen. Microbiol 4:330
    [Google Scholar]
  8. Powell J. F. 1951; Factors affecting the germination of a strain of Bacillus megatherium. J. gen. Microbiol 5:993
    [Google Scholar]
  9. Powell J. F. 1953; Isolation of dipicolinic acid (pyridine 2:6-dicarboxylic acid) from spores of Bacillus megatherium. Biochem. J 54:210
    [Google Scholar]
  10. Powell J. F., Strange R. E. 1953; Biochemical changes occurring during the germination of bacterial spores. Biochem. J 54:205
    [Google Scholar]
  11. Robinow C. F. 1951; Observations on the structure of Bacillus spores. J. gen. Microbiol 5:439
    [Google Scholar]
  12. Stewart B. T., Halvorson H. O. 1953; Studies on the spores of aerobic bacteria. I. The occurrence of alanine racemase. J. Bact 65:160
    [Google Scholar]
  13. Strange R. E., Powell J. F. 1954; Hexosamine-containing peptides in spores of Bacillus subtilis, B. megatherium, and B. cereus. Biochem. J 58:80
    [Google Scholar]
  14. Wade H. E., Morgan D. M. 1954; The analysis of adenosine triphosphate and adenosine diphosphate preparations by paper ionophoresis. Biochem. J 56:41
    [Google Scholar]
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