Summary: Sporulation of , NCTC 7582, in a complex medium was strongly stimulated by growth in mixed culture with a sporing strain of and with sporogenous and asporogenous strains of , NCTC 85. There was a similar, but less pronounced, effect with a variety of Gram-positive and Gram-negative organisms. The degree of sporulation of in these mixed cultures depended on () an external stimulus from the medium and () the previous cultural history of the inoculum upon which depended its ability to respond to the external stimulus. Sporulation of in pure culture was strongly stimulated by bicarbonate and ketoglutarate, suggesting that increased carbon dioxide production was the stimulating factor in mixed cultures.


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