@article{mbs:/content/journal/micro/10.1099/00221287-13-1-105, author = "Tomcsik, J. and Grace, Joyce B.", title = "Bacterial Cell Walls as Revealed by the Specific Cell-wall Reaction and by Direct Staining with Alcian Blue", journal= "Microbiology", year = "1955", volume = "13", number = "1", pages = "105-110", doi = "https://doi.org/10.1099/00221287-13-1-105", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-13-1-105", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Isolated cell walls of strains of Bacillus, Micrococcus and Caryophanon species obtained by mechanical disintegration, and in some instances by the action of trypsin, gave a specific cell-wall reaction, visible in the phase-contrast microscope on the addition of homologous antibody. The cell-wall structure, as revealed in the specific cell-wall reaction, is very similar to that observed in stained preparations. A method is described for staining the bacterial cell wall with Alcian blue without preliminary treatment with a mordant.", }