Tricarboxylic Acid Cycle Activity in Relation to Itaconic Acid Biosynthesis by Free

Abstract

Cell-free extracts of an itaconic acid producing strain of contained NADP-dependent isocitrate dehydrogenase activity but no NAD-dependent activity was detected. During the acid-producing phase the activity of the NADP-linked enzyme fell to one-eighth of its value during the growth phase, whereas citrate synthase activity increased. Intact mitochondria were isolated from growth and production phase mycelium. The preparation from the growth phase oxidized exogenous NADH and all tricarboxylic acid cycle intermediates tested. That from the production phase oxidized tricarboxylic acid cycle intermediates at a reduced rate but exogenous NADH oxidation remained high. [2-C]Acetate was incorporated into itaconic acid and over 90 % of the incorporated radioactivity was located in the methylene carbon. [1-C]-Acetate was a poorer precursor and did not preferentially label the methylene carbon. These results support the view that itaconic acid biosynthesis in occurs by decarboxylation of aconitic acid, the latter produced by the normal reactions of the tricarboxylic acid cycle.

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1983-09-01
2024-03-28
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References

  1. Bentley R., Thiessen C.P. 1957a; Biosynthesis of itaconic acid in Aspergillus terreus. I. Tracer studies with C14-labelled substrates. Journal of Biological Chemistry 226:673–687
    [Google Scholar]
  2. Bentley R., Thiessen C.P. 1957b; b)Biosynthesis of itaconic acid in Aspergillus terreus. II. Early stages in glucose dissimilation and the role of citrate. Journal of Biological Chemistry 226:689–701
    [Google Scholar]
  3. Bentley R., Thiessen C.P. 1957c; c)Biosynthesis of itaconic acid in Aspergillus terreus.III. The properties and reaction mechanism of c/s-aconitic decarboxylase. Journal of Biological Chemistry 226:703–720
    [Google Scholar]
  4. Bittner D.L., Manning J. 1967; Automated neocuproine glucose method: critical factors and normal values. In Automation in Analytical Chemistry Technicon Symposia, 1966 1 pp. 33–36 White Plains, New York: Mediad;
    [Google Scholar]
  5. Calam C.T., Oxford A.E., Raistrick H. 1939; The biochemistry of microorganisms. LXIII. Itaconic acid a metabolic product of a strain of Aspergillus terreus Thom. Biochemical Journal 33:1488–1495
    [Google Scholar]
  6. Chance B., Williams G.R. 1956; The respiratory chain and oxidative phosphorylation. Advances in Enzymology 17:65–134
    [Google Scholar]
  7. Corzo R.H. 1953 Biosynthesis of itaconic acid by Aspergillus terreus Ph.D. thesis Stanford University; U.S.A.:
    [Google Scholar]
  8. Corzo R.H., Tatum E.L. 1953; Biosynthesis of itaconic acid. Federation Proceedings 12:470
    [Google Scholar]
  9. Friedkin M. 1945; Determination of itaconic acid in fermentation liquors. Industrial and Engineering Chemistry. Analytical Edition 17:637–638
    [Google Scholar]
  10. Hankinson O., Cove D.J. 1974; Regulation of the pentose phosphate pathway in the fungus Aspergillus nidulans. . Journal of Biological Chemistry 249:2344–2353
    [Google Scholar]
  11. Jakubowska J. 1977; Itaconic and itatartaric acid biosynthesis. In Genetics and Physiology of Aspergillus, British Mycological Society Symposium Series no. 1 pp. 427–451 Edited by Smith J.E., Pateman J.A. London: Academic Press;
    [Google Scholar]
  12. Kinoshita K. 1931; Uber eine neue Aspergillus Art, A. itaconicus. . Botanical Magazine (Tokyo) 45:45–61
    [Google Scholar]
  13. Kornberg A., Pricer W.E. 1951; Di- and tri- phosphopyridine nucleotide isocitrate dehydrogenase in yeast. Journal of Biological Chemistry 189:123–136
    [Google Scholar]
  14. Lal M., Bhargava P.M. 1962; Reversal by pyruvate of fluoride inhibition in Aspergillus terreus. . Biochimica et biophysica acta 58:628–630
    [Google Scholar]
  15. La Nauze J.M. 1966; Aconitase and isocitric dehydrogenase of Aspergillus niger in relation to citric acid production. Journal of General Microbiology 44:73–81
    [Google Scholar]
  16. Mattey M. 1977; Citrate regulation of citric acid production in Aspergillus niger. . FEMS Microbiology Letters 2:71–74
    [Google Scholar]
  17. Miall L.M. 1978; Organic acids. In Primary Products of Metabolism pp. 47–119 Edited by Rose A.H. London: Academic Press;
    [Google Scholar]
  18. Mitsushima K., Shinmyo A., Enatsu U. 1976; Respiration activity in mitochondria prepared from cells of Candida lipolytica at different growth phases. Journal of Fermentation Technology 54:863–871
    [Google Scholar]
  19. Mitsushima K., Shinmyo A., Enatsu U. 1978; Control of citrate and 2-oxoglutarate formation in Candida lipolytica by adenine nucleotides. Biochimica et biophysica acta 538:481–492
    [Google Scholar]
  20. Nowakowska-Waszczuck A. 1973; Utilization of some tricarboxylic acid cycle intermediates by mitochondria and growing mycelium of Aspergillus terreus. . Journal of General Microbiology 79:19–29
    [Google Scholar]
  21. Ramakrishnan C.V., Martin S.M. 1955; Isocitrate dehydrogenase in Aspergillus niger. . Archives of Biochemistry and Biophysics 55:403–407
    [Google Scholar]
  22. Ramakrishnan C.V., Steel R., Lentz C.P. 1955; Mechanisms of citric acid formation and accumulation in Aspergillus niger. . Archives of Biochemistry and Biophysics 55:270–273
    [Google Scholar]
  23. Sanwal B.D., Slachow C.S. 1965; Allosteric activation of nicotinamide adenine dinucleotide specific isocitrate dehydrogenase of Neurospora. . Biochimica et biophysica acta 96:28–44
    [Google Scholar]
  24. Shimi I.R., Noureldein M.S. 1962; Biosynthesis of itaconic acid by Aspergillus terreus. . Archiv für Mikrobiologie 44:181–188
    [Google Scholar]
  25. Smith J.E., Nowakowska-Waszczuk A., Anderson J.G. 1974; Organic acid production by mycelial fungi. In Industrial Aspects of Biochemistry 1 pp. 297–317 Edited by Spencer B. Amsterdam: Elsevier;
    [Google Scholar]
  26. Srere P.A. 1969; Citrate synthase. Methods in Enzymology 13:4–5
    [Google Scholar]
  27. Stickland L.H. 1951; The determination of small quantities of bacteria by means of the biuret reaction. Journal of General Microbiology 5:698–703
    [Google Scholar]
  28. Watson K., Smith J.E. 1967; Oxidative phosphorylation and respiratory control in mitochondria from Aspergillus niger. . Biochemical Journal 104:332–339
    [Google Scholar]
  29. Watson K., Paton W., Smith J.E. 1969; Oxidative phosphorylation and respiratory control in mitochondria from Aspergillus oryzae. . Canadian Journal of Microbiology 15:975–977
    [Google Scholar]
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