1887

Abstract

In the tetrapolar basidiomycete a mutation in the factor impairs the vegetative growth of the hyphae. An ultrastructural study of the hyphae revealed an increased number of vacuoles in the subapical cells and defects in mitochondria in both apical and subapical cells. The ATP concentration in the hyphae and the specific activity of soluble inorganic pyrophosphatase (EC 3.6.1.1) were also observed to be decreased. These changes were interpreted as the result of non-specific hydrolytic activity caused by the mutation in the factor and originating in the cytoplasm but also affecting the mitochondria. The morphological and biochemical changes recorded in the mutant hyphae are comparable with those at the beginning of the mating of compatible homokaryons. At that time the breakdown of the cell components may be necessary to abolish the existing homokaryotic information and to control the direction of nuclear migration.

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1983-09-01
2022-01-17
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References

  1. Haylock R.W., Economou A., Casselton L.A. 1980; Dikaryon formation in Coprinus cinereus:selection and identification of B factor mutants. Journal of General Microbiology 121:17–26
    [Google Scholar]
  2. Heinonen J., Lahti R. 1981; A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Analytical Biochemistry 113:313–317
    [Google Scholar]
  3. Hoffman R.M., Raper J.R. 1971; Genetic restriction of energy conservation in Schizophyllum. . Science 171:418–419
    [Google Scholar]
  4. Hoffman R., Raper J.R. 1972; Lowered respiratory response to adenosine diphosphate of mitochondria isolated from a mutant B strain of Schizophyllum commune. . Journal of Bacteriology 110:780–781
    [Google Scholar]
  5. Hoffman R.M., Raper J.R. 1974; Genetic impairment of energy conservation in development of Schizophyllum: efficient mitochondria in energy-starved cells. Journal of General Microbiology 82:67–75
    [Google Scholar]
  6. Koltin Y., Flexer A.S. 1969; Alteration of nuclear distribution in fi-mutant strains of Schizophyllum commune. . Journal of Cell Science 4:739–749
    [Google Scholar]
  7. Kornberg A. 1962; On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions. In Horizons in Biochemistry pp. 251–264 Edited by Kasha H., Pullmann B. New York & London: Academic Press;
    [Google Scholar]
  8. Lahti R., Raudaskoski M. 1983; Soluble inorganic pyrophosphatase from Schizophyllum commune. . Folia microbiologica in the Press
    [Google Scholar]
  9. Niederpruem D.J. 1980; Direct studies of dikaryo-tization in Schizophyllum commune. I. Live intercellular nuclear migration patterns. Archives of Microbiology 128:162–171
    [Google Scholar]
  10. Parag Y. 1962; Mutations in the B incompatibility factor of Schizophyllum commune. . Proceedings of the National Academy of Sciences of the United States of America 48:743–750
    [Google Scholar]
  11. Raper C.A., Raper J.R. 1966; Mutations modifying sexual morphogenesis in Schizophyllum. . Genetics 51:1151–1168
    [Google Scholar]
  12. Raper J.R. 1966 Genetics of Sexuality in Higher Fungi New York: The Ronald Press Company;
    [Google Scholar]
  13. Raper J.R., Raudaskoski M. 1968; Secondary mutations at the BP incompatibility locus of Schizophyllum. . Heredity 23:109–117
    [Google Scholar]
  14. Raudaskoski M. 1972; Secondary mutations at the incompatibility locus and nuclear migration in the basidiomycete Schizophyllum commune. . Hereditas 72:175–182
    [Google Scholar]
  15. Raudaskoski M. 1973; Light and electron microscope study of unilateral mating between a secondary mutant and a wild-type strain of Schizophyllum commune. . Protoplasma 76:35–48
    [Google Scholar]
  16. Raudaskoski M. 1976; Acid phosphatase activity in the wild-type and 5-mutant hyphae of Schizophyllum commune. . Journal of General Microbiology 94:373–379
    [Google Scholar]
  17. Raudaskoski M. 1983; Unusual structure of nuclei in a 5-mutant strain of Schizophyllum commune with intercellular nuclear migration. Nordic Journal of Botany in the Press
    [Google Scholar]
  18. Raudaskoski M., Koltin Y. 1973; Ultrastructural aspects of a mutant of Schizophyllum communewith continuous nuclear migration. Journal of Bacteriology 116:981–988
    [Google Scholar]
  19. Raudaskoski M., Stamberg J., Bawnik N., Koltin Y. 1976; Mutational analysis of natural alleles at the 5 incompatibility factor of Schizophyllum commune: α and β6. Genetics 83:507–516
    [Google Scholar]
  20. Ross I.K. 1976; Nuclear migration rates in Coprinus congregatus: a new record?. Mycologia 68:418–422
    [Google Scholar]
  21. Salema R., Brandao I. 1973; The use of pipes buffer in the fixation of plant cells for electron microscopy. . Journal of Submicroscopic Cytology 5:79–96
    [Google Scholar]
  22. Snider P.J. 1968; Nuclear movements in Schizophyllum. . Symposia of the Society for Experimental Biology 22:261–283
    [Google Scholar]
  23. Snider P.J., Raper J.R. 1958; Nuclear migration in the basidiomycete Schizophyllum commune. . American Journal of Botany 45:538–546
    [Google Scholar]
  24. Spielmann H., Jacob-Müller U., Schulz P. 1981; Simple assay of 0·1-1·0 pmol of ATP, ADP and AMP in single somatic cells using purified luciferin luciferase. Analytical Biochemistry 113:172–178
    [Google Scholar]
  25. Takemaru T. 1961; Genetical studies on fungi. X. The mating system in hymenomycetes and its genetical mechanism. Biological Journal of Okayama University 7:133–211
    [Google Scholar]
  26. Thore A. 1979; Technical aspects of the bioluminescent firefly luciferase assay of ATP. Science Tools 26:30–34
    [Google Scholar]
  27. Watrud L.S., Ellingboe A.H. 1973a; Use of cobalt as a mitochondrial vital stain to study cytoplasmic exchange in matings of the basidiomycete Schizophyllum commune. . Journal of Bacteriology 115:1151–1158
    [Google Scholar]
  28. Watrud L.S., Ellingboe A.H. 1973b; Cobalt as a mitochondrial density marker in a study of cytoplasmic exchange during mating of Schizophyllum commune. . Journal of Cell Biology 59:127–133
    [Google Scholar]
  29. Wessels J.G.H. 1969; Biochemistry of sexual morphogenesis in Schizophyllum commune: effect of mutations affecting the incompatibility system on cell-wall metabolism. Journal of Bacteriology 98:697–704
    [Google Scholar]
  30. Wessels J.G.H. 1978; Incompatibility factors and the control of biochemical processes. In Genetics and Morphogenesis in the Basidiomycetes pp. 81–104 Edited by Schwalb M.N., Miles P.G. New York: Academic Press;
    [Google Scholar]
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