1887

Abstract

Summary: A high molecular weight protein antigen, designated P1, has been isolated from the culture fluid of chemostat-grown strain Ingbritt and shown to be free of other antigens including glucosyltransferase. Antiserum against the protein was used in rocket immunoelectro-phoresis to confirm and extend the previous observation that there were major differences in the amount of the protein produced under different growth conditions. Physico-chemical and serological studies indicated that protein P1 was indistinguishable from antigens B, I II and IF isolated in other laboratories. Mammalian tissue cross-reactivity of protein P1 was demonstrated by binding of antiserum to P1 to sections of normal rabbit tissues, particularly heart. There was also a statistically significant increase in the number of mononuclear leucocytes in heart tissue of rabbits which had been injected with protein P1, when compared with the levels in control uninjected rabbits; injection with whole cells of Ingbritt did not produce this effect.

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/content/journal/micro/10.1099/00221287-129-9-2779
1983-09-01
2019-12-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-9-2779
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