1887

Abstract

A high molecular weight protein antigen, designated P1, has been isolated from the culture fluid of chemostat-grown strain Ingbritt and shown to be free of other antigens including glucosyltransferase. Antiserum against the protein was used in rocket immunoelectro-phoresis to confirm and extend the previous observation that there were major differences in the amount of the protein produced under different growth conditions. Physico-chemical and serological studies indicated that protein P1 was indistinguishable from antigens B, I II and IF isolated in other laboratories. Mammalian tissue cross-reactivity of protein P1 was demonstrated by binding of antiserum to P1 to sections of normal rabbit tissues, particularly heart. There was also a statistically significant increase in the number of mononuclear leucocytes in heart tissue of rabbits which had been injected with protein P1, when compared with the levels in control uninjected rabbits; injection with whole cells of Ingbritt did not produce this effect.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-9-2779
1983-09-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/9/mic-129-9-2779.html?itemId=/content/journal/micro/10.1099/00221287-129-9-2779&mimeType=html&fmt=ahah

References

  1. Beachey E.H., Seyer J.M., Dale J.B., Simpson W.A., Kang A.H. 1981; Type-specific protective immunity evoked by synthetic peptide of Streptococcus pyogenes M protein. Nature, London 292:457–459
    [Google Scholar]
  2. Campbell L.K., Knox K.W., Wicken A.J. 1978; Extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilute acid. Infection and Immunity 22:842–851
    [Google Scholar]
  3. Carlsson J. 1970; A levansucrase from Streptococcus mutans. Caries Research 4:97–113
    [Google Scholar]
  4. Closs O., Harboe M., Wassum A.M. 1975; Cross-reactions between mycobacteria. Scandinavian Journal of Immunology Suppl. 4 2:173–185
    [Google Scholar]
  5. Colman G., Cohen B. 1979; Immunisation of monkeys (Macaca fascicularis) with Streptococcus mutans. In Pathogenic Streptococci pp. 214–215 Edited by Parker M.T. Chertsey: Reedbooks;
    [Google Scholar]
  6. Dische Z. 1955; New color reactions for determinations of sugars in polysaccharides. Methods of Biochemical Analysis 2:313–358
    [Google Scholar]
  7. Ferretti J.J., Shea C., Humphrey M.W. 1980; Cross-reactivity of Streptococcus mutans antigens and human heart tissue. Infection and Immunity 30:69–73
    [Google Scholar]
  8. Fukui K., Moriyama T., Miyake Y., Mizutani K., Tanaka O. 1982; Purification and properties of glucosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans. Infection and Immunity 37:1–9
    [Google Scholar]
  9. Gibbons M.N. 1955; The determination of methyl pentoses. Analyst 80:268–276
    [Google Scholar]
  10. Hardy L., Jacques N.A., Forester H., Campbell L.K., Knox K.W., Wicken A.J. 1981; Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production, and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture. Infection and Immunity 31:78–87
    [Google Scholar]
  11. Hughes M. 1981; Cross-reactions of Streptococcus mutans with heart tissue antigens. In Pathogenic Streptococci pp. 222–223 Edited by Parker M.T. Chertsey: Reedbooks;
    [Google Scholar]
  12. Hughes M., Machardy S.M., Sheppard A.J., Woods N.C. 1980; Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infection and Immunity 27:576–588
    [Google Scholar]
  13. Hughes M., Machardy S.M., Sheppard A.J. 1981; Binding of Streptococcus mutans antigens to mammalian cardiac tissue in vitro. Annual Meeting oj the British Division of the International Association for Dental Research Abstract 208
    [Google Scholar]
  14. Hughes M., Machardy S.M., Sheppard A.J. 1983; Manufacture and control of a dental caries vaccine for parenteral administration to man. In Chemistry and Biology of Glucosyl Transferases in the Press London: Information Retrieval Inc.;
    [Google Scholar]
  15. Jacques N.A., Hardy L., Campbell L.K., Knox K.W., Evans J.D., Wicken A.J. 1979a; Effect of carbohydrate source and growth conditions on the production of lipoteichoic acid by Streptococcus mutans Ingbritt. Infection and Immunity 26:1079–1087
    [Google Scholar]
  16. Jacques N.A., Hardy L., Knox K.W., Wicken A.J. 1979b; Effect of growth conditions on the formation of extracellular lipoteichoic acid by Streptococcus mutans BHT. Infection and Immunity 25:75–84
    [Google Scholar]
  17. Janda J., Work E. 1971; A colorimetric estimation of lipopolysaccharides. FEBS Letters 16:343–345
    [Google Scholar]
  18. Knox K.W., Forester H., Hardy L., Wicken A.J. 1981; Effect of growth conditions on the production of extracellular proteins by oral streptococci. In Basic Concepts of Streptococci and Streptococcal Diseases pp. 144–145 Edited by Holm S.E., Christensen P. Chertsey: Reedbooks;
    [Google Scholar]
  19. Lehner T., Russell M.W., Caldwell J. 1980; Immunisation with a purified protein from Streptococcus mutans against dental caries in rhesus monkeys. Lancet 1:995–996
    [Google Scholar]
  20. Lehner T., Russell M.W., Caldwell J., Smith R. 1982; Immunisation with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infection and Immunity 34:407–415
    [Google Scholar]
  21. Liljemark W.F., Schauer S.V. 1975; Studies on the bacterial components which bind Streptococcus sanguis and Streptococcus mutans to hydroxyapatite. Archives of Oral Biology 20:609–615
    [Google Scholar]
  22. Mccabe M.M., Hamelik R.M., Smith E.E. 1977; Purification of dextran-binding protein from cariogenic Streptococcus mutans. Biochemical and Physiological Research Communications 78:273–278
    [Google Scholar]
  23. Mcghee J.R., Michalek S.M. 1981; Immunobiology of dental caries: microbial aspects and local immunity. Annual Review of Microbiology 35:595–638
    [Google Scholar]
  24. Mollison P.L. 1975; Blood transfusion in clinical medicine. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  25. Nisengard R.J., Stinson M.W., Albini B. 1983; Kidney and heart pathology in rabbits immunized with Streptococcus mutans. Annual Meeting of the American Association for Dental Research Abstract 210
    [Google Scholar]
  26. Rijn I., Van Der, Bleiweis A.S., Zabriskie J.B. 1976; Antigens in Streptococcus mutans crossreactive with human heart muscle. Journal of Dental Research special issue C 55:C59–C64
    [Google Scholar]
  27. Russell M.W., Lehner T. 1978; Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Archives of Oral Biology 23:7–15
    [Google Scholar]
  28. Russell M.W., Bergmeier L., Zanders E.D., Lehner T. 1980; Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease resistant component. Infection and Immunity 28:486–493
    [Google Scholar]
  29. Russell R.R.B. 1979a; Glucan-binding proteins of Streptococcus mutans serotype c. Journal of General Microbiology 112:197–201
    [Google Scholar]
  30. Russell R.R.B. 1979b; Wall-associated protein antigens of Streptococcus mutans. Journal of General Microbiology 114:109–115
    [Google Scholar]
  31. Russell R.R.B., Colman G. 1981; Immunisation of monkeys (Macaca fascicularis) with purified Streptococcus mutans glucosyl transferase. Archives of Oral Biology 26:23–28
    [Google Scholar]
  32. Russell R.R.B., Beighton D., Cohen B. 1982; Immunisation of monkeys (Macaca fascicularis) with antigens purified from Streptococcus mutans. British Dental Journal 152:81–84
    [Google Scholar]
  33. Russell R.R.B., Peach S.L., Colman G., Cohen B. 1983; Antibody responses to antigens of Streptococcus mutans in monkeys (Macaca fascicularis) immunized against dental caries. Journal of General Microbiology 129:865–875
    [Google Scholar]
  34. Stinson M.W., Nisengard R.J., Bergey E.J. 1980; Binding of streptococcal antigens to muscle tissue in vitro. Infection and Immunity 27:604–613
    [Google Scholar]
  35. Walker G.J., Murray V.L., Morrey-Jones J.G. 1980; Regulation of dextranase synthesis by Streptococcus mutans. FEBS Letters 115:206–208
    [Google Scholar]
  36. Zanders E.D., Lehner T. 1981; Separation and characterization of a protein antigen from cells of Streptococcus mutans. Journal of General Microbiology 122:217–225
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-9-2779
Loading
/content/journal/micro/10.1099/00221287-129-9-2779
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error