1887

Abstract

The enterotoxigenic strains 1676, 1706, 1751 and KEC96a, which do not produce fimbrial adhesive antigens of the K88, K99 or 987P antigen type reacted both and with antiserum to F41 fimbriae in an indirect immunofluorescent antibody technique. Antiserum used to demonstrate material B, an adhesive antigen thought to mediate the adhesive and mannose-resistant (MR) haemagglutinating properties of strains 1676, 1706 and 1751, reacted with an F41 strain. The antiserum also inhibited the MR haemagglutinating activity of F41 antigen and gave an anionic precipitation line in immunoelectrophoresis experiments with an extract containing F41 antigen.

The MR haemagglutinating properties of an antigen extract containing material B from strain 1706 was neutralized by antiserum to F41 fimbriae and by OK antisera to strains that produce both F41 and K99 fimbriae. These sera also gave an anionic precipitation line with the MR haemagglutinin from strain 1706 and the MR haemagglutinin gave a line of identity with F41 in gel diffusion experiments with antiserum to F41 fimbriae. OK antisera to K99 F41 bacteria and OK antisera to K88 bacteria and 987P bacteria did not react with this haemagglutinin.

Transmission electron microscopy on the ileum of newborn gnotobiotic piglets infected with strain 1706 showed irregular, poorly defined filamentous material surrounding some, though not all, bacteria but regular fimbrial structures were not visible. Fine thread-like material was visible surrounding most of the bacteria in ultra thin sections from a piglet infected with an strain that produces both F41 and K99. Staining with ruthenium red suggested. that this material was associated with polysaccharide.

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/content/journal/micro/10.1099/00221287-129-9-2753
1983-09-01
2024-11-07
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References

  1. Awad-Masalmeh M., Moon H.W., Runnels P.L., Schneider R.A. 1982; Pilus production, hemagglutination, and adhesion by porcine strains of enterotoxigenicEscherichia coli lacking K88, K99 and 987P antigens. Infection and Immunity 35:305–313
    [Google Scholar]
  2. De Graaf F.K., Roorda I. 1982; Production, purification and characterization of the fimbrial adhesive antigen F41 isolated from the calf entero-pathogenic Escherichia coli strain B41M. Infection and Immunity 36:754–758
    [Google Scholar]
  3. Guinee P.A.M., Jansen W.H., Agterberg C.M. 1976; Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with enterotoxigenicity. Infection and Immunity 13:1369–1377
    [Google Scholar]
  4. Hadad J.J.B., Gyles C.L. 1982; Scanning and transmission electron microscopic study of the small intestine of colostrum-fed calves infected with selected strains of Escherichia coli. American Journal of Veterinary Research 43:41–49
    [Google Scholar]
  5. Moon H.W., Isaacson R.E., Pohlenz J. 1979; Mechanisms of association of enteropathogenic Escherichia coli with intestinal epithelium. American Journal of Clinical Nutrition 32:191–127
    [Google Scholar]
  6. Moon H.W., Kohler E.M., Schneider R.A., Whipp S.C. 1980; Prevalence of pilus antigens, enterotoxin types and enteropathogenicity among K88-negative enteroxigenic Escherichia coli from neonatal pigs. Infection and Immunity 27:222–230
    [Google Scholar]
  7. Morris J.A., Hussaini S.N. 1974; Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis. Journal of Hygiene 73:425–432
    [Google Scholar]
  8. Morris J.A., Stevens A.E., Sojka W.J. 1977; Preliminary characterization of cell-free K99 antigen isolated from Escherichia coli B41. Journal of General Microbiology 99:353–358
    [Google Scholar]
  9. Morris J.A., Stevens A.E., Sojka W.J. 1978; Anionic and cationic components of the K99 surface antigen from Escherichia coli B41. Journal of General Microbiology 107:173–175
    [Google Scholar]
  10. Morris J.A., Thorns C.J., Scott A.C., Sojka W.J., Wells G.A.H. 1982; Adhesion in vitroand in vivo associated with an adhesive antigen (F41) produced by a K99 mutant of the reference strain Escherichia coli B41. Infection and Immunity 36:1146–1153
    [Google Scholar]
  11. Morris J.A., Thorns C.J., Sojka W.J. 1980; Evidence for two adhesive antigens on the K99 reference strain Escherichia coli B41. Journal of General Microbiology 118:107–113
    [Google Scholar]
  12. Sellwood R., Gibbons R.A., Jones G.W., Rutter J.M. 1975; Adhesion of enteropathogenic Escherichia coli to pig intestinal brush border.The existence of two pig phenotypes. Journal of Medical Microbiology 8:405–411
    [Google Scholar]
  13. Sojka W.J. 1965 Escherichia coli in Domestic Animals and Poultry. Famhamn Royal:: Common wealth Agricultural Bureaux.;
    [Google Scholar]
  14. Sojka W.J, Wray C., Morris J.A. 1978; Passive protection of lambs against experimental enteric colibacillosis by colostral transfer of antibodies from K99-vaccinated ewes. Journal of Medical Microbiology 11:493–499
    [Google Scholar]
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