1887

Abstract

Spores formed by carrying the mutation are lysozyme-sensitive and germination-defective. The coats of these spores lack a number of polypeptides normally found in the spore coat, and four additional polypeptides are present that are not normally found in the coats of wild-type spores. The mutation also prevents the synthesis (at about stage V) and the incorporation into the spore outer layers, of an intracellular protease (protease B, approx. mol wt. of monomer 25000). A partial revertant of a strain carrying was isolated and this had an additional mutation in a locus distinct from . The suppressing mutation restored the protease activity, but not the germination defect. It partly restored resistance to lysozyme and the spores formed had apparently normal coat protein composition except for the absence of one polypeptide (mol wt. 36000).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-9-2727
1983-09-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/9/mic-129-9-2727.html?itemId=/content/journal/micro/10.1099/00221287-129-9-2727&mimeType=html&fmt=ahah

References

  1. Coote J.G. 1972; Sporulation in Bacillus subtilis.Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  2. Dion P., Mandelstam J. 1980; Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation. Journal of Bacteriology 141:786–792
    [Google Scholar]
  3. Glenn A.R., Mandelstam J. 1971; Sporulation in Bacillus subtilis 168.Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
    [Google Scholar]
  4. Goldman R.C., Tipper D.J. 1981; Coat protein synthesis during sporulation of Bacillus subtilis:immunological detection of soluble precursors to the 12,200-dalton spore coat protein. Journal of Bacteriology 147:1040–1048
    [Google Scholar]
  5. Hranueli D., Piggot P.J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis speculation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  6. Janssen F.W., Lund A.J., Anderson L.E. 1958; Colorimetric assay for dipicolinic acid in bacterial spores. Science 127:26–21
    [Google Scholar]
  7. Jenkinson H.F. 1981; Germination and resistance defects in spores of a Bacillus subtilis mutant lacking a coat polypeptide. Journal of General Microbiology 127:81–91
    [Google Scholar]
  8. Jenkinson H.F. 1983; Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis. Journal of General Microbiology 129:1945–1958
    [Google Scholar]
  9. Jenkinson H.F., Kay D., Mandelstam J. 1980; Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them. Journal of Bacteriology 141:793–805
    [Google Scholar]
  10. Jenkinson H.F., Sawyer W.D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. Journal of General Microbiology 123:1–16
    [Google Scholar]
  11. Kerjan P., Keryer E., Szulmajster J. 1979; Characterization of a thermosensitive sporulation mutant of Bacillus subtilis affected in the structural gene of an intracellular protease. European Journal of Biochemistry 98:353–362
    [Google Scholar]
  12. Lacks S.A., Springhorn S.S. 1980; Renaturation of enzyme after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Journal of Biological Chemistry 255:7467–7473
    [Google Scholar]
  13. Laemmli U.K., Favre M. 1973; Maturation of the head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  14. Lafferty E., Moir A. 1977; Further studies on conditional germination mutants of Bacillus subtilis168. In Spore Research 1976 pp. 87–105 Barker A.N., Wolf J., Ellar D.J., Dring G.J., Gould G.W. Edited by London:: Academic Press.;
    [Google Scholar]
  15. Moir A. 1981; Germination properties of a spore coat-defective mutant of Bacillus subtilis. Journal of Bacteriology 146:1106–1116
    [Google Scholar]
  16. Moir A., Lafferty E., Smith D.A. 1979; Genetic analysis of spore germination mutants of Bacillus subtilis 168: the correlation of phenotype with map location. Journal of General Microbiology 111:165–180
    [Google Scholar]
  17. OhnÉ M., Rutberg B., Hoch J.A. 1973; Genetic and biochemical characterization of mutants of Bacillus subtilis defective in succinate dehydrogenase. Journal of Bacteriology 115:738–745
    [Google Scholar]
  18. Piggot P.J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  19. Powell J.F., Hunter J.R. 1955; Spore germination in the genus Bacillus: the modification of germination requirements as a result of preheating. Journal of General Microbiology 13:59–67
    [Google Scholar]
  20. Sadoff H.L. 1966; Glucose dehydrogenase-soluble.I.Bacillus cereus. Methods in Enzymology 9:103–107
    [Google Scholar]
  21. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis: étude génétique et physiologique. Colloques internationaux du centre national de la recherche scientifique 124:553–563
    [Google Scholar]
  22. Srivastava O.P., Aronson A.I. 1981; Isolation and characterization of a unique protease from sporulating cells of Bacillus subtilis. Archives of Microbiology 129:227–232
    [Google Scholar]
  23. Sterlini J.M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  24. Ward J.B. Jr Zahler S.A. 1973; Genetic studies of leucine biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:719–726
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-9-2727
Loading
/content/journal/micro/10.1099/00221287-129-9-2727
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error