1887

Abstract

The biosynthesis of wax esters by the psychrophilic bacterium has been investigated using radio-gas chromatographic analysis of the constituent fatty acid and fatty alcohol moieties. Degradation studies, using a;-oxidation, and the kinetics of labelling from radioactive precursors show that wax ester fatty alcohols are derived from fatty acids. Based on different labelling patterns in wax esters and phospholipids formed from acetate or a saturated fatty acid supplied exogenously, two pathways of wax ester biosynthesis are proposed. It is suggested that wax esters can be derived in the main from either a cytoplasmic or a membrane-bound pool of fatty acyl-coenzyme A thioesters and alcohols, depending on whether the fatty acid is synthesized endogenously or is supplied exogenously.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-8-2641
1983-08-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/8/mic-129-8-2641.html?itemId=/content/journal/micro/10.1099/00221287-129-8-2641&mimeType=html&fmt=ahah

References

  1. Dean R.B., Dixon W.J. 1951; Simplified statistics for small numbers of observations. Analytical Chemistry 23:636–638
    [Google Scholar]
  2. Foot M. 1980 Studies on a bacterial Δ9;desaturase. Ph.D. thesis. University of Wales, U.K.:
    [Google Scholar]
  3. Foot M., Jeffcoat R., Russell N.J. 1983; Some properties, including the substrate in vivo, of the Δ9;- desaturase in Micrococcus cryophilus. Biochemical Journal 209:345–353
    [Google Scholar]
  4. Garbus J., Deluca H.F., Loomans M.E., Strong F.M. 1963; The rapid incorporation of phosphate into mitochondrial lipids. Journal of Biological Chemistry 238:59–63
    [Google Scholar]
  5. Grigor M.R., Harris E.L. 1977; Wax ester synthesis in the mouse preputial gland tumour. Biochimica et biophysica acta 488:121–127
    [Google Scholar]
  6. Kates M. 1972 Techniques of Lipidology. Isolation, Analysis and Identification of Lipids, 1st edn. p. 519 Amsterdam: North-Holland Publishing Co;
    [Google Scholar]
  7. Klein K., Steinberg R., Fiethen B., Overath P. 1971; Fatty acid degradation in Escherichia coli. An inducible system for uptake of fatty acids and further characterization of old mutants. European Journal of Biochemistry 19:442–450
    [Google Scholar]
  8. Kolattukudy P.E. 1967; Mechanisms of synthesis of waxy esters in Broccoli (Brassica oleracea). Biochemistry 6:2705–2717
    [Google Scholar]
  9. Kolattukudy P.E. 1970; Reduction of fatty acid to alcohols by cell free preparations of Euglena gracilis. Biochemistry 9:1095–1102
    [Google Scholar]
  10. Ohlrogge J.B., Pollard M.R., Stumpf P.K. 1978; Studies on biosynthesis of waxes by developing jojoba seed tissue. Lipids 13:203–210
    [Google Scholar]
  11. Pollard M.R., Mckeon T., Gupta L.M., Stumpf P.K. 1979; Studies on biosynthesis of waxes by developing jojoba seed. II. The demonstration of wax biosynthesis by cell-free homogenates. Lipids 14:651–662
    [Google Scholar]
  12. Raetz C.R.H. 1978; Enzymology, genetics and regulation of membrane phospholipid synthesis in Esherichia coli. Microbiological Reviews 42:614–659
    [Google Scholar]
  13. Russell N.J. 1974; The lipid composition of the psychrophilic bacterium Micrococcus cryophilus. Journal of General Microbiology 80:217–225
    [Google Scholar]
  14. Russell N.J., Volkman J.K. 1980; The effect of growth temperature on wax ester composition in the psychrophilic bacterium Micrococcus cryophilus ATCC 15174. Journal of General Microbiology 118:131–141
    [Google Scholar]
  15. Sandercock S.P., Russell N.J. 1980; The elongation of exogenous fatty acids and the control of phospholipid acyl chain length in Micrococcus cryophilus. Biochemical Journal 188:585–592
    [Google Scholar]
  16. Sargent J.R., Lee R.F., Nevenzel J.C. 1976; Marine waxes. In Chemistry and Biochemistry of Natural Waxes pp. 49–91 Edited by Kolattukudy P. E. Amsterdam: Elsevier;
    [Google Scholar]
  17. Shine W.E., Mancha M., Stumpf P.K. 1976; Fat metabolism in higher plants: the function of acyl thioesterases in the metabolism of acyl coenzyme A and acyl-acyl carrier proteins. Archives of Biochemistry and Biophysics 172:110–116
    [Google Scholar]
  18. Wang L., Takayama K., Goldman D.S., Schnoes H.K. 1972; Synthesis of alcohol and wax ester by a cell-free system in Mycobacterium tuberculosis. Biochimica et biophysica acta 260:41–48
    [Google Scholar]
  19. Wu X-Y., Moreau R.A., Stumpf P.K. 1981; Studies of biosynthesis of waxes by developing jojoba seed. III. Biosynthesis of wax esters from acyl-CoA and long chain alcohols. Lipids 16:897–902
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-8-2641
Loading
/content/journal/micro/10.1099/00221287-129-8-2641
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error